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Invitrogen
ATP5A1 Monoclonal Antibody (7H10BD4F9)
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FIGURE: 1 / 28
ATP5A1 Antibody (459240) in ICC/IF
Bovine pulmonary artery endothelial cells: Mitochondria were labeled using an anti-OxPhos Complex V subunit a, mouse IgG2b monoclonal antibody (Product # 459240) prelabeled with the Zenon® Alexa Fluor® 555 Mouse IgG2b Labeling Kit (Product # Z-25205). Tubulin was detected with an anti-a-tubulin mouse IgG2b monoclonal antibody prelabeled with the Zenon® Alexa Fluor® 488 Mouse IgG2b Labeling Kit (Product # Z-25202), and The nucleus was stained with DAPI (Product # D1306, D3571, D21490).
Product Details
459240
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Human,
Mouse,
Rat
Published species
Bovine,
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG2b, kappa
Class
Monoclonal
Type
Antibody
Clone
7H10BD4F9
Immunogen
Bovine Complex V
Conjugate
Form
Liquid
Concentration
1.0 mg/mL
Purification
IgG fraction
Storage buffer
HEPES buffered saline
Contains
0.02% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Use Isolated mitochondria from Human, Rat, Bovine and Mouse Heart, and HepG2, Cultured Human embryonic lung-derived fibroblasts (strain MRC5), HL-60 cells for positive controls.
Target Information
The ATP5F1A gene (ATP Synthase F1 Subunit Alpha) is located on chromosome 18q21.33 and encodes a critical component of the mitochondrial ATP synthase complex, specifically the F1 catalytic core. The ATP synthase complex is essential for cellular energy production, facilitating the conversion of ADP to ATP during oxidative phosphorylation. The ATP5F1A subunit is responsible for the catalysis of ATP synthesis driven by the proton gradient across the inner mitochondrial membrane. Dysfunctions or mutations in ATP5F1A can result in mitochondrial disorders and are linked to various metabolic and neurodegenerative diseases. Understanding the structure and function of ATP5F1A is vital for comprehending the bioenergetics of cellular respiration and has implications in studying mitochondrial pathologies.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: alpha subunit ATP synthase isoform precursor (EC 3.6.1.34); ATP synthase alpha chain, mitochondrial; ATP synthase alpha subunit; ATP synthase alpha subunit precursor; ATP synthase F(1) complex subunit alpha, mitochondrial; ATP synthase F1 subunit alpha; ATP synthase subunit alpha, mitochondrial; ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1, cardiac muscle; ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, isoform 1; ATP sythase (F1-ATPase) alpha subunit; epididymis secretory sperm binding protein Li 123m; iso; mitochondrial ATP synthetase, oligomycin-resistant; mitochondrial H+-ATP synthase alpha subunit; modifier of Min 2; unnamed protein product
Gene Aliases: AI035633; AL022851; AL023067; ATP5A; ATP5A1; ATP5A2; ATP5AL2; ATP5F1A; ATPM; COXPD22; D18Ertd206e; hATP1; HEL-S-123m; MC5DN4; MC5DN4A; MC5DN4B; MOM2; OMR; ORM
UniProt ID: (Human) P25705, (Bovine) P19483, (Rat) P15999, (Mouse) Q03265
Entrez Gene ID: (Human) 498, (Bovine) 282578, (Rat) 65262, (Mouse) 11946
nucleotide binding
protease binding
RNA binding
protein binding
ATP binding
ATPase activity
adenyl ribonucleotide binding
MHC class I protein binding
ADP binding
angiostatin binding
proton-transporting ATP synthase activity, rotational mechanism
poly(A) RNA binding
proton-transporting ATPase activity, rotational mechanism
hydrolase activity, acting on acid anhydrides, catalyzing transmembrane movement of substances
ATP synthase
negative regulation of endothelial cell proliferation
lipid metabolic process
ATP biosynthetic process
ion transport
response to muscle activity
ATP synthesis coupled proton transport
mitochondrial ATP synthesis coupled proton transport
positive regulation of blood vessel endothelial cell migration
response to ethanol
ATP metabolic process
cellular response to dexamethasone stimulus
cellular response to nitric oxide
hydrogen ion transmembrane transport
ATP hydrolysis coupled proton transport
transport
embryo development
proton transport
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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