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Invitrogen
MAP2 Monoclonal Antibody (HM-2)
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FIGURE: 1 / 19
MAP2 Antibody (MA1-25043) in ICC/IF
Immunofluorescence analysis of Microtubule-associated protein 2 was performed using 70% confluent log phase SH-SY5Y cells differentiated to neurons (Retinoic acid 10 uM for 5 days). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with MAP2 Monoclonal Antibody (HM-2) (Product # MA1-25043) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG... View More
Product Details
MA1-25043
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Avian,
Bovine,
Chicken,
Human,
Mouse,
Rat
Published species
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
HM-2
Immunogen
Rat brain microtubule-associated proteins (MAPs).
Conjugate
Form
Liquid
Concentration
2 mg/mL
Amount
100 µg
Purification
purified
Storage buffer
PBS, pH 7.4
Contains
15mM sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recommended positive controls: rat brain.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
MAP2 (Microtubule Associated Protein 2) exists in two high molecular weight forms (MAP2a & MAP2b) and a low molecular weight form. The expression of MAP2 is developmentally regulated and its multiple forms arise by alternative splicing of a single gene. MAP2 is involved in microtubule assembly, which is an essential step in neurogenesis. The products of similar genes in rat and mouse are neuron-specific cytoskeletal proteins that are enriched in dendrites, implicating a role in determining and stabilizing dendritic shape during neuron development. MAP2 is useful in studies of neuron structure in normal and malignant brain tissue as well as in degenerative diseases such as Alzheimer's Disease (AD).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: AC091465.1; DKFZp686I2148; MAP 2; MAP-2; map-2a; map2ab; microtubule associated protein 2; microtubule associated protein 2C; Microtubule-associated protein 2; microtubule-associated protein-2; OTTHUMP00000163916; OTTHUMP00000208143; unnamed protein product
Gene Aliases: G1-397-34; MAP-2; MAP2; MAP2A; MAP2B; MAP2C; MAP2R; Mtap-2; Mtap2; repro4
UniProt ID: (Human) P11137, (Mouse) P20357, (Rat) P15146
Entrez Gene ID: (Bovine) 281294, (Human) 4133, (Chicken) 424001, (Mouse) 17756, (Rat) 25595
microtubule bundle formation
axonogenesis
dendrite development
cellular response to organic substance
central nervous system neuron development
neuron projection development
response to estradiol
response to progesterone
dendrite morphogenesis
microtubule cytoskeleton organization
negative regulation of axon extension
regulation of microtubule polymerization
negative regulation of microtubule polymerization
regulation of protein localization
positive regulation of anterograde dense core granule transport
regulation of organelle transport along microtubule
positive regulation of anterograde synaptic vesicle transport
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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