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Invitrogen
CD31 Monoclonal Antibody (TLD-3A12)
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FIGURE: 1 / 6
CD31 Antibody (MA1-81051) in WB
Western blot was performed using Anti-CD31 Monoclonal Antibody (TLD-3A12) (Product # MA1-81051) and a 130kDa band corresponding to Platelet endothelial cell adhesion molecule was observed across cell lines tested. Membrane enriched extracts (40 µg lysate) of HUVEC (Lane 1), HEL 92.1.7 (Lane 2), Jurkat (Lane 3), MDA-MB-231 (Lane 4), THP-1 (Lane 5) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blot... View More
Product Details
MA1-81051
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Rhesus monkey,
Rat
Published species
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
TLD-3A12
Immunogen
Activated, Lewis rat derived microglial cells.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
250 µg
Purification
Protein A
Storage buffer
PBS
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Clone TLD-3A12 has been shown to partially block the proliferative response of antigen-specific CD4+ T cells to antigen-presenting cells and relevant antigen.
For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.
Mouse anti Rat CD31 antibody, clone TLD-3A12 recognizes rat PECAM-1 (CD31), a 661 amino acid type 1 transmembrane protein expressed primarily on endothelial cells, platelets and leucocytes.
Target Information
CD31 (platelet endothelial cell adhesion molecule-1, PECAM-1) is an inhibitory coreceptor involved in regulation of T cell and B cell signaling by a dual immunoreceptor tyrosine-based inhibitory motif (ITIM) that upon associated kinases-mediated phosphorylation provide docking sites for protein-tyrosine phosphatases. CD31 is expressed ubiquitously within the vascular compartment and is located mainly at junctions between adjacent cells. N-terminal Ig-like domain of CD31 is responsible for its homophilic binding, which plays an important role in cell-cell interactions. CD31 is a multifunctional molecule with diverse roles in modulation of integrin-mediated cell adhesion, transendothelial migration, angiogenesis, apoptosis, negative regulation of immunoreceptor signaling, autoimmunity, macrophage phagocytosis, IgE-mediated anaphylaxis and thrombosis. It is one of key regulatory molecules in vascular system.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CD31; PECAM-1; Platelet endothelial cell adhesion molecule
Gene Aliases: CD31; Pecam; Pecam1
UniProt ID: (Rat) Q3SWT0
Entrez Gene ID: (Rhesus monkey) 718302, (Rat) 29583
positive regulation of leukocyte migration
phagocytosis
cell adhesion
Rho protein signal transduction
regulation of cell migration
negative regulation of actin filament polymerization
response to cytokine
negative regulation of GTPase activity
wound healing
positive regulation of tyrosine phosphorylation of Stat5 protein
negative regulation of peptidyl-tyrosine phosphorylation
diapedesis
glomerular endothelium development
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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