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Invitrogen

CD3e Monoclonal Antibody (4E2)

View all (580) CD3 antibodies

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Product Details

MA5-15627

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

Flow Cytometry (Flow)

1:200-1:400
-

ELISA (ELISA)

1:10,000
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

4E2

Immunogen

Purified recombinant fragment of CD3E expressed in E. Coli.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

ascites

Contains

0.03% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10978861

Product Specific Information

MA5-15627 targets CD3E in indirect ELISA, FACS and WB applications and shows reactivity with Human samples.

The MA5-15627 immunogen is purified recombinant fragment of CD3E expressed in E. Coli.

MA5-15627 detects CD3E which has a predicted molecular weight of approximately 23kDa.

Target Information

The CD3 complex, composed of gamma, delta, epsilon, and zeta subunits, is essential for the assembly, trafficking, and surface expression of the T cell receptor (TCR) complex. These subunits are structurally related members of the immunoglobulin superfamily and are encoded by closely linked genes on human chromosome 11. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells, but not on B or NK cells. The CD3 subunits play a crucial role in transducing antigen-recognition signals into the cytoplasm of T cells. The cytoplasmic tails of CD3 subunits contain a double tyrosine-based motif that associates with cytoplasmic signal transduction molecules, mediating T cell activation through the TCR. Crosslinking of the TCR initiates intracellular biochemical pathways that result in cellular activation, proliferation, and potentially growth arrest and cell survival. CD3 is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes, and Purkinje cells in the cerebellum. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases. Defects in the CD3 gene are associated with CD3 immunodeficiency, highlighting its importance in immune function and regulation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: CD247; CD247 antigen, zeta subunit; CD3 antigen, delta subunit; CD3 delta; CD3 TCR complex; CD3-epsilon; CD3d; CD3d antigen, delta polypeptide (TiT3 complex); CD3d molecule, delta (CD3-TCR complex); CD3e; CD3e antigen, epsilon polypeptide (TiT3 complex); CD3e molecule, epsilon (CD3-TCR complex); CD3g; CD3g antigen, gamma polypeptide (TiT3 complex); CD3g molecule, epsilon (CD3-TCR complex); CD3g molecule, gamma (CD3-TCR complex); CD3Z antigen, zeta polypeptide (TiT3 complex); CD3zeta chain; component of the TCR/CD3 complex; FLJ18683; Leu-4; OKT3, delta chain; T cell antigen receptor complex epsilon subunit of T3; T-cell antigen receptor complex, epsilon subunit of T3; T-cell antigen receptor complex, gamma subunit of T3; T-cell antigen receptor complex, zeta subunit of CD3; T-cell receptor T3 delta chain; T-cell receptor T3 gamma chain; T-cell receptor T3 zeta chain; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 delta chain; T-cell surface glycoprotein CD3 epsilon chain; T-cell surface glycoprotein CD3 gamma chain; T-cell surface glycoprotein CD3 zeta chain; T3/TCR complex; TCR zeta; TCR zeta chain; tissue factor-targeting CAR1 dimer; tissue factor-targeting CAR1 monomer; unnamed protein product

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Gene Aliases: CD247; CD3-DELTA; CD3-GAMMA; CD3-ZETA; CD3D; CD3DELTA; CD3E; CD3epsilon; CD3G; CD3GAMMA; CD3H; CD3Q; CD3Z; CD3ZETA; IMD17; IMD18; IMD19; IMD25; T3D; T3E; T3G; T3Z; TCRE; TCRZ

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UniProt ID: (Human) P20963, (Human) P04234, (Human) P07766, (Human) P09693

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Entrez Gene ID: (Human) 919, (Human) 915, (Human) 916, (Human) 917

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Function(s)
transmembrane signaling receptor activity protein binding identical protein binding protein homodimerization activity protein heterodimerization activity protein tyrosine kinase binding SH3 domain binding protein kinase binding receptor signaling complex scaffold activity T cell receptor binding
Process(es)
adaptive immune response immune system process cell surface receptor signaling pathway Fc-gamma receptor signaling pathway gamma-delta T cell activation alpha-beta T cell activation T cell receptor signaling pathway protein oligomerization protein-containing complex assembly positive regulation of protein localization to cell surface positive thymic T cell selection establishment or maintenance of cell polarity protein transport T cell activation regulation of lymphocyte apoptotic process positive regulation of cell-matrix adhesion positive regulation of T cell anergy T cell anergy transmembrane receptor protein tyrosine kinase signaling pathway signal complex assembly G-protein coupled receptor signaling pathway smoothened signaling pathway regulation of signal transduction positive regulation of gene expression negative regulation of gene expression dendrite development calcium-mediated signaling cerebellum development T cell costimulation positive regulation of interferon-gamma production positive regulation of interleukin-2 production positive regulation of interleukin-4 production positive regulation of cell-cell adhesion mediated by integrin CD4-positive, alpha-beta T cell proliferation T cell proliferation positive regulation of T cell proliferation regulation of apoptotic process positive regulation of MAPK cascade negative thymic T cell selection negative regulation of smoothened signaling pathway lymphocyte activation positive regulation of calcium-mediated signaling positive regulation of T cell activation apoptotic signaling pathway positive regulation of CD4-positive, alpha-beta T cell proliferation
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