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Invitrogen
TRPM7 Monoclonal Antibody (S74)
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FIGURE: 1 / 4
TRPM7 Antibody (MA5-27620) in IHC (P)
Immunohistochemistry analysis of TRPM7 in paraffin-embedded mouse hippocampus. Samples were incubated with TRPM7 monoclonal antibody (Product # MA5-27620) using a dilution of 1:1000 (1 hour at RT) and followed by Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) and Mayer Hematoxylin (purple/blue) at a dilution of 250-500 µl.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-27620
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Mouse,
Pig,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
S74
Immunogen
Fusion protein amino acids 1817-1863 (C- terminus) of mouse TrpM7
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.09% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
1 µg/mL of MA5-27620 was sufficient for detection of TrpM7 in 10 µg of COS cell lysate transiently transfected with TprM7 by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 220kDa in extracts of cells transiently transfected with TrpM7. No cross-reactivity against TrpM6.
Target Information
TRPCs, mammalian homologs of the Drosophila transient receptor potential (trp) protein, are ion channels that are thought to mediate capacitative calcium entry into the cell. TRP-PLIK is a protein that is both an ion channel and a kinase. As a channel, it conducts calcium and monovalent cations to depolarize cells and increase intracellular calcium. As a kinase, it is capable of phosphorylating itself and other substrates. The kinase activity is necessary for channel function, as shown by its dependence on intracellular ATP and by the kinase mutants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: ChaK1; melanoma alpha-kinase; Channel kinase 1; channel-kinase 1; FLJ20117; ion channel; protein kinase; TRP-PLIK; long transient receptor potential channel 7; LTRPC ion channel family member 7; member of LTRPC ion channel family; tr; transient receptor potential M7; transient receptor potential-phospholipase C-interacting kinase; transient receptor potential-related protein, ChaK; TRMP7; TRP PLIK; trp-related; unnamed protein product
Gene Aliases: 2310022G15Rik; 4833414K03Rik; 5033407O22Rik; ALSPDC; CHAK; CHAK1; Ltpr7; LTrpC-7; LTRPC7; TRP-PLIK; TRPPLIK
UniProt ID: (Human) Q96QT4, (Mouse) Q923J1, (Rat) Q925B3
Entrez Gene ID: (Human) 54822, (Mouse) 58800, (Rat) 679906
actin binding
protein kinase activity
protein serine/threonine kinase activity
calcium channel activity
zinc ion transmembrane transporter activity
protein binding
ATP binding
calcium ion transmembrane transporter activity
magnesium ion transmembrane transporter activity
kinase activity
myosin binding
protein serine kinase activity
cation channel activity
cation transmembrane transporter activity
protein phosphorylation
calcium ion transport
zinc II ion transport
memory
magnesium ion homeostasis
cellular magnesium ion homeostasis
magnesium ion transport
calcium-dependent cell-matrix adhesion
metal ion transport
actomyosin structure organization
positive regulation of apoptotic process
protein autophosphorylation
protein tetramerization
protein homotetramerization
necroptotic process
calcium ion transmembrane transport
zinc II ion transmembrane transport
cation transmembrane transport
magnesium ion transmembrane transport
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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