Search
Invitrogen
TRPM7 Monoclonal Antibody (S74), PerCP
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Additional Information:
{{banner.description}}
FIGURE: 1 / 4
TRPM7 Antibody (MA5-45425) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis using human neuroblastoma cells. Fixation involved 4% PFA for 15 min. Samples were incubated with TrpM7 monoclonal antibody (Product # MA5-45425) at 1:50 for overnight at 4°C with slow rocking, followed by AlexaFluor 488 at 1:1,000 for 1 hour at RT. Counterstain used was Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) TrpM7 antibody (D) Composite.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-45425
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
S74
Immunogen
Fusion protein amino acids 1817-1863 (C- terminus) of mouse TrpM7
Conjugate
PerCP
PerCP
PerCP
Excitation/Emission Max
482/675 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
95.64mM phosphate/2.48mM MES, pH 7.4, with 0.5M EDTA
Contains
no preservative
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
1 µg/mL of MA5-45425 was sufficient for detection of TrpM7 in 10 µg of COS cell lysate transiently transfected with TprM7 by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 220kDa in extracts of cells transiently transfected with TrpM7. No cross-reactivity against TrpM6.
Target Information
TRPCs, mammalian homologs of the Drosophila transient receptor potential (trp) protein, are ion channels that are thought to mediate capacitative calcium entry into the cell. TRP-PLIK is a protein that is both an ion channel and a kinase. As a channel, it conducts calcium and monovalent cations to depolarize cells and increase intracellular calcium. As a kinase, it is capable of phosphorylating itself and other substrates. The kinase activity is necessary for channel function, as shown by its dependence on intracellular ATP and by the kinase mutants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: ChaK1; melanoma alpha-kinase; Channel kinase 1; channel-kinase 1; FLJ20117; ion channel; protein kinase; TRP-PLIK; long transient receptor potential channel 7; LTRPC ion channel family member 7; member of LTRPC ion channel family; tr; transient receptor potential M7; transient receptor potential-phospholipase C-interacting kinase; transient receptor potential-related protein, ChaK; TRMP7; TRP PLIK; trp-related; unnamed protein product
Gene Aliases: 2310022G15Rik; 4833414K03Rik; 5033407O22Rik; ALSPDC; CHAK; CHAK1; Ltpr7; LTrpC-7; LTRPC7; TRP-PLIK; TRPPLIK
UniProt ID: (Human) Q96QT4, (Mouse) Q923J1, (Rat) Q925B3
Entrez Gene ID: (Human) 54822, (Mouse) 58800, (Rat) 679906
actin binding
protein kinase activity
protein serine/threonine kinase activity
calcium channel activity
zinc ion transmembrane transporter activity
protein binding
ATP binding
calcium ion transmembrane transporter activity
magnesium ion transmembrane transporter activity
kinase activity
myosin binding
protein serine kinase activity
cation channel activity
cation transmembrane transporter activity
protein phosphorylation
calcium ion transport
zinc II ion transport
memory
magnesium ion homeostasis
cellular magnesium ion homeostasis
magnesium ion transport
calcium-dependent cell-matrix adhesion
metal ion transport
actomyosin structure organization
positive regulation of apoptotic process
protein autophosphorylation
protein tetramerization
protein homotetramerization
necroptotic process
calcium ion transmembrane transport
zinc II ion transmembrane transport
cation transmembrane transport
magnesium ion transmembrane transport
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support