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Invitrogen
GRP78 Monoclonal Antibody (1H11-1H7)
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FIGURE: 1 / 12
GRP78 Antibody (MA5-27686) in ICC/IF
Immunofluorescence analysis of GRP78 was performed using 70% confluent log phase MCF7 cells and MCF7 cells treated with 1 µM of Thapsigargin for 24 hours. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with GRP78 Mouse Monoclonal Antibody (1H11-1H7) (Product # MA5-27686) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then with Goat anti-Mouse IgG (H+L) Superclonal™ Secondar... View More
Product Details
MA5-27686
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Fungi,
Hamster,
Human,
Mouse,
Rabbit,
Rhesus monkey,
Rat,
Xenopus,
Yeast
Published species
Bovine,
Human
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
1H11-1H7
Immunogen
Full-length his-tagged human GRP78
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.09% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
0.5 µg/mL of MA5-27686 was sufficient for detection of Grp78 in 10 µg of rat tissue lysate by ECL immunoblot analysis.|Detects approximately 78kDa.
Target Information
GRP78 is a 78 kDa glucose regulated protein that belongs to the family of heat shock proteins that include HSP70. GRP78 is a resident protein of the endoplasmic reticulum (ER) and associates transiently with a variety of newly synthesized secretory and membrane proteins. GRP78 may also interact with mutant and misfolded proteins, marking them for degradation. The highly conserved sequence Lys-Asp-Glu-Leu (KDEL) is present at the C-terminus of GRP78 and other resident ER proteins including glucose regulated protein 94 (GRP94) and protein disulfide isomerase (PDI). The KDEL signal is recognized by the KDEL receptor and this interaction ensures recycling of escaped ER proteins back to the ER. GRP78's involvement in correct folding of proteins and degradation of misfolded proteins is via its interaction with DNAJC10, probably to facilitate the release of DNAJC10 from its substrate. GRP78 is critical for maintenance of cell homeostasis and the prevention of apoptosis. GRP78 protein levels have been shown to be a reliable biomarker of hypoglycemia and serves a neuroprotective function in neurons exposed to glutamate and oxidative stress. GRP78 levels are reduced in the brains of Alzheimer's Disease patients, and decreased expression of GRP78 is found associated with missense mutations in the human presenilin-1 (PS1) gene. Further, the induction of the GRP78 protein has been associated with the development of drug-resistance to antitumor drugs.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 78 kDa glucose-regulated protein; 78 kDa glucose-regulated protein homolog; Binding-immunoglobulin protein; BiP; BiP protein; chaperone; Endoplasmic reticulum chaperone BiP; endoplasmic reticulum lumenal Ca(2+)-binding protein grp78; epididymis secretory sperm binding protein Li 89n; FLJ26106; glucose regulated protein, 78 kDa; glucose-regulated protein; glucose-regulated protein (grp78); glucose-regulated protein, 78kDa; GRP 78; GRP-78; heat shock 70 kDa protein 5; heat shock 70 kDa protein 5a; heat shock 70kD protein 5; heat shock 70kD protein 5 (glucose-regulated protein, 78kD); heat shock 70kDa protein 5 (glucose-regulated protein); heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa); heat shock protein 5; Heat shock protein 70 family protein 5; Heat shock protein family A member 5; heavy-chain binding protein BiP; HSP70 family protein 5; HSPA 5; immunoglobulin binding protein; Immunoglobulin heavy chain-binding protein; Immunoglobulin heavy chain-binding protein homolog; Steroidogenesis-activator polypeptide; unnamed protein product; XAP-1 antigen
Gene Aliases: AL022860; AU019543; baffled; BIP; BiP/Grp78; D2Wsu141e; D2Wsu17e; GRP78; HEL-S-89n; Hsce70; HSPA5; hspa5a; I79_019946; J1248; KAR2; mBiP; SEZ-7; Sez7; SSD1; XAP-1; XELAEV_180384511mg; YJL034W
nucleotide binding
calcium ion binding
protein binding
ATP binding
hydrolase activity
ATPase activity
enzyme binding
protein domain specific binding
protein serine/threonine kinase inhibitor activity
heat shock protein binding
ubiquitin protein ligase binding
ribosome binding
protein binding involved in protein folding
cadherin binding
unfolded protein binding
chaperone binding
misfolded protein binding
ATP-dependent protein folding chaperone
glycoprotein binding
ER overload response
activation of signaling protein activity involved in unfolded protein response
cerebellum structural organization
cerebellar Purkinje cell layer development
substantia nigra development
positive regulation of cell migration
negative regulation of transforming growth factor beta receptor signaling pathway
positive regulation of protein ubiquitination
response to endoplasmic reticulum stress
maintenance of protein localization in endoplasmic reticulum
cellular response to glucose starvation
negative regulation of apoptotic process
proteolysis involved in cellular protein catabolic process
cellular response to antibiotic
cellular response to interleukin-4
toxin transport
positive regulation of embryonic development
translational initiation
protein folding
endoplasmic reticulum unfolded protein response
posttranslational protein targeting to membrane, translocation
negative regulation of protein complex assembly
protein folding in endoplasmic reticulum
IRE1-mediated unfolded protein response
PERK-mediated unfolded protein response
ERAD pathway
protein refolding
positive regulation of transcription from RNA polymerase II promoter
regulation of protein folding in endoplasmic reticulum
regulation of ATF6-mediated unfolded protein response
regulation of IRE1-mediated unfolded protein response
negative regulation of IRE1-mediated unfolded protein response
regulation of PERK-mediated unfolded protein response
negative regulation of PERK-mediated unfolded protein response
karyogamy involved in conjugation with cellular fusion
SRP-dependent cotranslational protein targeting to membrane, translocation
response to unfolded protein
ER-associated ubiquitin-dependent protein catabolic process
cellular response to unfolded protein
chaperone mediated protein folding requiring cofactor
fungal-type cell wall beta-glucan biosynthetic process
protein localization to endoplasmic reticulum
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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