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Invitrogen

GRP78 Monoclonal Antibody (1H11-1H7), FITC

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GRP78 Monoclonal Antibody (1H11-1H7), FITC

Product Details

MA5-45277

Applications
Tested Dilution
Publications

Western Blot (WB)

1:2,000
-

Immunocytochemistry (ICC/IF)

1:100
-

ELISA (ELISA)

1:10,000
-
Product Specifications

Species Reactivity

Bovine, Fungi, Hamster, Human, Mouse, Non-human primate, Rabbit, Rat, Xenopus

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

1H11-1H7

Immunogen

His-tagged human GRP78
View immunogen

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra spectra

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

9.09mM sodium bicarbonate/PBS, pH 7.4, with 640.91mM DMSO, 136.36mM ethanolamine

Contains

no preservative

Storage conditions

4°C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2931731

Product Specific Information

0.5 µg/mL of MA5-45277 was sufficient for detection of Grp78 in 10 µg of rat tissue lysate by ECL immunoblot analysis.|Detects approximately 78kDa.

Target Information

GRP78 is a 78 kDa glucose regulated protein that belongs to the family of heat shock proteins that include HSP70. GRP78 is a resident protein of the endoplasmic reticulum (ER) and associates transiently with a variety of newly synthesized secretory and membrane proteins. GRP78 may also interact with mutant and misfolded proteins, marking them for degradation. The highly conserved sequence Lys-Asp-Glu-Leu (KDEL) is present at the C-terminus of GRP78 and other resident ER proteins including glucose regulated protein 94 (GRP94) and protein disulfide isomerase (PDI). The KDEL signal is recognized by the KDEL receptor and this interaction ensures recycling of escaped ER proteins back to the ER. GRP78's involvement in correct folding of proteins and degradation of misfolded proteins is via its interaction with DNAJC10, probably to facilitate the release of DNAJC10 from its substrate. GRP78 is critical for maintenance of cell homeostasis and the prevention of apoptosis. GRP78 protein levels have been shown to be a reliable biomarker of hypoglycemia and serves a neuroprotective function in neurons exposed to glutamate and oxidative stress. GRP78 levels are reduced in the brains of Alzheimer's Disease patients, and decreased expression of GRP78 is found associated with missense mutations in the human presenilin-1 (PS1) gene. Further, the induction of the GRP78 protein has been associated with the development of drug-resistance to antitumor drugs.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 78 kDa glucose-regulated protein; Binding-immunoglobulin protein; BiP; BiP protein; chaperone; Endoplasmic reticulum chaperone BiP; endoplasmic reticulum lumenal Ca(2+)-binding protein grp78; epididymis secretory sperm binding protein Li 89n; FLJ26106; glucose regulated protein, 78 kDa; glucose-regulated protein; glucose-regulated protein (grp78); glucose-regulated protein, 78kDa; GRP 78; GRP-78; heat shock 70 kDa protein 5; heat shock 70 kDa protein 5a; heat shock 70kD protein 5; heat shock 70kD protein 5 (glucose-regulated protein, 78kD); heat shock 70kDa protein 5 (glucose-regulated protein); heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa); heat shock protein 5; Heat shock protein 70 family protein 5; Heat shock protein family A member 5; heavy-chain binding protein BiP; HSP70 family protein 5; HSPA 5; immunoglobulin binding protein; Immunoglobulin heavy chain-binding protein; Steroidogenesis-activator polypeptide; unnamed protein product; XAP-1 antigen

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Gene Aliases: AL022860; AU019543; baffled; BIP; BiP/Grp78; D2Wsu141e; D2Wsu17e; GRP78; HEL-S-89n; Hsce70; HSPA5; hspa5a; I79_019946; mBiP; SEZ-7; Sez7; XAP-1; XELAEV_180384511mg

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UniProt ID: (Human) P11021, (Bovine) Q0VCX2, (Rat) P06761, (Mouse) P20029, (Chinese hamster) G3I8R9, (Xenopus) Q91883

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Entrez Gene ID: (Human) 3309, (Rabbit) 100008764, (Bovine) 415113, (Rat) 25617, (Mouse) 14828, (Chinese hamster) 100689305, (Xenopus) 397850

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Function(s)
nucleotide binding calcium ion binding protein binding ATP binding hydrolase activity ATPase activity enzyme binding protein domain specific binding protein serine/threonine kinase inhibitor activity heat shock protein binding ubiquitin protein ligase binding ribosome binding protein binding involved in protein folding cadherin binding unfolded protein binding chaperone binding misfolded protein binding ATP-dependent protein folding chaperone glycoprotein binding
Process(es)
ER overload response activation of signaling protein activity involved in unfolded protein response cerebellum structural organization cerebellar Purkinje cell layer development substantia nigra development positive regulation of cell migration negative regulation of transforming growth factor beta receptor signaling pathway positive regulation of protein ubiquitination response to endoplasmic reticulum stress maintenance of protein localization in endoplasmic reticulum cellular response to glucose starvation negative regulation of apoptotic process proteolysis involved in cellular protein catabolic process cellular response to antibiotic cellular response to interleukin-4 toxin transport positive regulation of embryonic development translational initiation protein folding endoplasmic reticulum unfolded protein response posttranslational protein targeting to membrane, translocation negative regulation of protein complex assembly protein folding in endoplasmic reticulum IRE1-mediated unfolded protein response PERK-mediated unfolded protein response ERAD pathway protein refolding positive regulation of transcription from RNA polymerase II promoter regulation of protein folding in endoplasmic reticulum regulation of ATF6-mediated unfolded protein response regulation of IRE1-mediated unfolded protein response negative regulation of IRE1-mediated unfolded protein response regulation of PERK-mediated unfolded protein response negative regulation of PERK-mediated unfolded protein response
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