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Invitrogen
Phospho-SMAD2 (Ser250) Recombinant Rabbit Monoclonal Antibody (10M1E3)
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FIGURE: 1 / 1
Phospho-SMAD2 (Ser250) Antibody (MA5-35859) in WB
Western blot analysis of Phospho-SMAD2 (Ser250) in extracts of various cell lines. Samples were incubated with Phospho-SMAD2 (Ser250) Monoclonal antibody (Product # MA5-35859) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) secondary antibody at a dilution of 1:10,000. 293T cells were treated by PMA/TPA (200 nM) at 37°C for 30 minutes after serum-starvation overnight or treated by Ca... View More
Product Details
MA5-35859
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
10M1E3
Immunogen
A synthetic phosphorylated peptide around S250 of human Smad2 (Q15796)
Conjugate
Form
Liquid
Concentration
0.57 mg/mL
Amount
57 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol, 0.05% BSA
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: ELSPTT
Target Information
SMAD2 (MADH2, MAD2) regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. SMAD2 interacts with the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation into the nucleus is a central event in TGF beta signaling. Phosphorylation of threonine 8 in the calmodulin-binding region of the MH1 domain by extracellular signal regulated kinase 1 (ERK1) enhances SMAD2 transcriptional activity, which is negatively regulated by calmodulin. In response to the TGF-beta signal, SMAD2 is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. SMAD2 can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively spliced transcript variants encoding the SMAD2 protein have been observed.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: hMAD-2; JV18-1; MAD; MAD homolog 2; Mad protein homolog; Mad-related protein 2; MGC22139; MGC34440; mMad2; mother against DPP homolog 2; Mothers against decapentaplegic homolog 2; mothers against DPP homolog 2; pSMAD2; Sma- and Mad-related protein 2; SMAD; SMAD 2; SMAD family member 2; SMAD, mothers against DPP homolog 2; Smad2Deltaexon3; unnamed protein product
Gene Aliases: 7120426M23Rik; CHTD8; hMAD-2; hSMAD2; JV18; JV18-1; LDS6; MADH2; MADR2; mMad2; Smad-2; SMAD2
UniProt ID: (Human) Q15796, (Mouse) Q62432
Entrez Gene ID: (Human) 4087, (Mouse) 17126
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
core promoter proximal region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
chromatin binding
double-stranded DNA binding
transcription factor activity, sequence-specific DNA binding
transforming growth factor beta receptor binding
protein binding
phosphatase binding
ubiquitin protein ligase binding
type I transforming growth factor beta receptor binding
identical protein binding
SMAD binding
metal ion binding
tau protein binding
RNA polymerase II sequence-specific DNA binding transcription factor binding
co-SMAD binding
I-SMAD binding
R-SMAD binding
disordered domain specific binding
DNA-binding transcription factor binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
transcription factor binding
transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity
activating transcription factor binding
protein homodimerization activity
protein heterodimerization activity
DNA-binding transcription factor
gene-specific transcriptional regulator
ureteric bud development
in utero embryonic development
endoderm formation
mesoderm formation
regionalization
determination of left/right asymmetry in lateral mesoderm
aortic valve morphogenesis
pulmonary valve morphogenesis
endocardial cushion morphogenesis
transcription, DNA-templated
regulation of transcription, DNA-templated
transforming growth factor beta receptor signaling pathway
zygotic specification of dorsal/ventral axis
gastrulation
pattern specification process
endoderm development
mesoderm development
heart development
cell proliferation
anatomical structure morphogenesis
response to glucose
post-embryonic development
embryonic pattern specification
anterior/posterior pattern specification
positive regulation of gene expression
negative regulation of gene expression
positive regulation of epithelial to mesenchymal transition
regulation of transforming growth factor beta receptor signaling pathway
insulin secretion
cell differentiation
negative regulation of ossification
lung development
positive regulation of BMP signaling pathway
pancreas development
primary miRNA processing
activin receptor signaling pathway
organ growth
intracellular signal transduction
nodal signaling pathway
cell fate commitment
negative regulation of cell differentiation
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
paraxial mesoderm morphogenesis
developmental growth
embryonic foregut morphogenesis
embryonic cranial skeleton morphogenesis
regulation of multicellular organismal process
pericardium development
SMAD protein signal transduction
mesenchyme development
trophoblast cell migration
secondary palate development
response to cholesterol
response to transforming growth factor beta
odontoblast differentiation
transcription from RNA polymerase II promoter
protein phosphorylation
common-partner SMAD protein phosphorylation
SMAD protein complex assembly
negative regulation of cell proliferation
signal transduction involved in regulation of gene expression
regulation of binding
palate development
positive regulation of nodal signaling pathway involved in determination of lateral mesoderm left/right asymmetry
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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