Search
Invitrogen
MDC1 Monoclonal Antibody (P2B11), PerCP
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Additional Information:
{{banner.description}}
FIGURE: 1 / 3
MDC1 Antibody (MA5-45286) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis using mouse fibroblast cells. Fixation involved 4% Formaldehyde for 15 min at RT. Samples were incubated with MDC1 monoclonal antibody (Product # MA5-45286) at 1:100 for 60 min at RT, followed by Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain used was DAPI (blue) nuclear stain at 1:5,000 for 5 min RT. Localization: Nucleus. Magnification: 60X.
Product Details
MA5-45286
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Chimpanzee,
Human,
Mouse
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
P2B11
Immunogen
GST-tagged recombinant protein corresponding to mouse MDC1 at and around the N-terminus
Conjugate
Excitation/Emission Max
482/675 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
95.64mM phosphate/2.48mM MES, pH 7.4, with 0.5M EDTA
Contains
no preservative
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
0.5 µg/mL of MA5-45286 was sufficient for detection of MDC1 in 10 µg of HeLa cell lysate by ECL immunoblot analysis.|Detects approximately 184kDa. This antibody recognizes MDC1 at and around the N-terminus.
Target Information
MDC1 participates in the early response to DNA damage. It is thought to be a member of the BRCT (BRCA1 C-terminus) super family of nuclear proteins. It contains an N-terminus forkhead-associated (FHA) motif, two C-terminus BRCT motifs and 13 internal repetitions of a 41 amino acid sequence.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: DAAP-285E11.7; DKFZp781A0122; homologue to Drosophila photoreceptor protein calphotin; KIAA0170; mediation of DNA damage checkpoint 1; mediator of DNA damage checkpoint 1; Mediator of DNA damage checkpoint protein 1; MGC166888; Nuclear factor with BRCT domains 1; nuclear protein; unnamed protein product
Gene Aliases: 6820401C03; AA413496; KIAA0170; MDC1; mKIAA0170; NFBD1
UniProt ID: (Human) Q14676, (Chimpanzee) Q7YR40, (Mouse) Q5PSV9
Entrez Gene ID: (Human) 9656, (Chimpanzee) 471959, (Mouse) 240087
DNA replication checkpoint
DNA repair
chromatin organization
cellular response to DNA damage stimulus
intra-S DNA damage checkpoint
protein K6-linked ubiquitination
positive regulation of double-strand break repair via homologous recombination
protein localization to site of double-strand break
cell cycle
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support