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Invitrogen

ATG5 Polyclonal Antibody

2 Published Figures
4 References
View all (52) ATG5 antibodies

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ATG5 Polyclonal Antibody

Product Details

PA1-46178

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500
View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:400
-

Immunocytochemistry (ICC/IF)

1:250
-

Flow Cytometry (Flow)

Assay-Dependent
-

ELISA (ELISA)

Assay-Dependent
-

Immunoprecipitation (IP)

1:10-1:500
-

Radioimmune Assays (RIA)

Assay-Dependent
-

Proximity Ligation Assay (PLA) (PLA)

Assay-Dependent
-

Immunomicroscopy (IM)

Assay-Dependent
-
Product Specifications

Species Reactivity

Bovine, Fruit fly, Fish, Guinea pig, Human, Mouse, Non-human primate, Pig, Reptile, Rat, Xenopus, Zebrafish

Published species

Human, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide of an N-terminal region of the human ATG5 protein (within residues 1-50).
3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Amount

100 µg

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2274520

Product Specific Information

In Western Blot, a band is seen ~56 kDa representing the ATG5-ATG12 complex, the molecular weight of human ATG5 is ~33 kDa.

Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

Suggested positive control: antigen standard for ATG5 (transient overexpression lysate), mouse wildtype ES cell lysate.

Target Information

ATG5 (Autophagy Related 5) is an important element for autophagy and may play an important role in the apoptotic process. ATG5 is also involved in other cellular processes that include mitochondrial quality control after oxidative damage, negative regulation of the innate anti-viral immune response, lymphocyte development and proliferation, MHC II antigen presentation, and adipocyte differentiation. Following conjugation to ATG12, the conjugate participates in the formation of autophagosome. ATG5 contributes to autophagic cell death by interacting with Fas-associated protein with death domain (FADD). The ATG5-ATG12 conjugate forms a cup-shaped isolation membrane that then detaches from the membrane immediately before or after autophagosome formation is completed. APG5 may play a role in the apoptotic process, possibly within the modified cytoskeleton. Further, APG5 expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. The APG5-APG12 conjugate also associates with innate immune response proteins such as RIG-I and VISA (also known as IPS-1), inhibiting type I interferon production and permitting viral replication in host cells. Diseases associated with ATG5 dysfunction include spinocerebellar ataxia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: alternatively spliced; APG 5; APG5 autophagy 5 like (APG5L); APG5 autophagy 5-like; APG5-like; Apoptosis-specific protein; Apoptosis-specific protein (ASP); ATG5 autophagy related 5 homolog; Atg5-PA; autophagy 5-like protein; Autophagy protein 5; autophagy related 5-like protein; autophagy-related 5; autophagy-specific gene 5; CG1643-PA; LD34980p; similar to S. cerevisiae ATG5 autophagy related 5; unnamed protein product

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Gene Aliases: 2010107M05Rik; 3110067M24Rik; APG5; APG5-LIKE; APG5L; ASP; Atg-5; ATG5; Atg5l; AW319544; C88337; CG1643; DmAtg5; Dmel\CG1643; Dmel_CG1643; hAPG5; Paddy; SCAR25; XELAEV_18027227mg; zgc:100934

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UniProt ID: (Pig) Q3MQ04, (Human) Q9H1Y0, (Bovine) Q3MQ24, (Fruit fly) Q9W3R7, (Mouse) Q99J83, (Rat) Q3MQ06

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Entrez Gene ID: (Pig) 100739102, (Human) 9474, (Bovine) 532686, (Fruit fly) 31666, (Guinea pig) 100735170, (Mouse) 11793, (Rat) 365601, (Zebrafish) 494180, (Xenopus) 443756

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Function(s)
protein binding Atg8 ligase activity membrane trafficking regulatory protein
Process(es)
immune system process autophagy apoptotic process regulation of cilium assembly autophagosome assembly mitophagy blood vessel remodeling regulation of cytokine production involved in immune response chromatin organization cellular response to nitrogen starvation cellular response to starvation response to xenobiotic stimulus response to fungus response to iron(II) ion positive regulation of autophagy cardiac muscle cell apoptotic process negative regulation of cardiac muscle cell apoptotic process macroautophagy protein ubiquitination antigen processing and presentation of endogenous antigen negative regulation of protein ubiquitination negative regulation of type I interferon production piecemeal microautophagy of nucleus aggrephagy negative stranded viral RNA replication vasodilation negative regulation of apoptotic process negative regulation of programmed cell death post-translational protein modification negative thymic T cell selection innate immune response negative regulation of innate immune response otolith development negative regulation of defense response to virus negative regulation of phagocytosis regulation of release of sequestered calcium ion into cytosol defense response to virus establishment of localization in cell ventricular cardiac muscle cell development heart contraction chaperone-mediated autophagy positive regulation of stress granule assembly mucus secretion positive regulation of mucus secretion cellular response to nitrosative stress axonal transport regulation of postsynaptic membrane neurotransmitter receptor levels postsynaptic modulation of chemical synaptic transmission regulation of autophagosome maturation response to fluoride negative regulation of autophagic cell death positive regulation of viral translation regulation of reactive oxygen species metabolic process response to drug protein lipidation involved in autophagosome assembly autophagy of host cells involved in interaction with symbiont negative regulation of reactive oxygen species metabolic process negative regulation of histone H4-K16 acetylation mitophagy C-terminal protein lipidation larval midgut cell programmed cell death nucleophagy glycophagy regulation of cytokine secretion involved in immune response positive regulation of mitophagy nervous system development heart development regulation of skeletal muscle tissue regeneration
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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