Invitrogen

IRAK1 Polyclonal Antibody

Name: IRAK1 Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-19855

Advanced Verification

4 Published Figures

4 References

View all (38) IRAK1 antibodies

Datasheet

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Protocols

Questions & Answers

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Datasheet

Certificates

Protocols

Questions & Answers

Tech Support

Datasheet

Certificates

Protocols

Questions & Answers

Tech Support

Additional Information:

FIGURE: 1 / 6

IRAK1 Antibody (PA5-19855) in WB

Western blot was performed using Anti-IRAK1 Polyclonal Antibody (Product # PA5-19855) and an 80 kDa band corresponding to IRAK1 was observed across cell lines tested. Whole cell extracts (30 µg lysate) of HEK-293 (Lane 1), LNCaP (Lane 2), HeLa (Lane 3), PC-3 (Lane 4), K-562 (Lane 5), U-2 OS (Lane 6) and T-47D (Lane 7) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB2100... View More

IRAK1 Antibody in Western Blot, Immunoprecipitation (WB, IP)

Product Image Gallery

IRAK1 Antibody — Figure 5 Analysis of E2 signal transduction. ( a ) Western blotting of A549, MCF-10A, and 293T cell lysates showing IL-1R expression; cytoplasmic and membrane components of the cell lysates were separated. A549 cells were included as a positive control for IL-1R1 expression, and 293T cells were used as a negative control. ( b ) Western blotting of MCF-10A cells showing phospho-IRAK1 (left) and IRAK1 (right) following treatment with 100 ng/ml GST-IL-1beta for 0-30 min. ( c ) Western blotting of MCF-10A cells probed to examine phospho-p38(Thr180/Tyr182) and p38 after adding 100 ng/ml GST-IL-1beta for 0-60 min. ( d ) Western blotting of MCF-10A cells probed to examine phospho-p38 (Thr180/Tyr182) and p38 after adding 1 ng/ml GST-IL-1beta for 0-60 min. ( e ) Western blotting of MCF-10A cells showing phospho-JNK(Thr183/Tyr185) and JNK following treatment with 100 ng/ml GST-IL-1beta for 0-60 min. ( f ) Western blotting of MCF-10A cells showing phospho-IkB (Ser32) and IkB following treatment with 100 ng/ml GST-IL-1beta for 0-30 min. ( g ) MMP-3 activity assay of MCF-10A cells following treatment with 100 ng/ml GST-IL-1beta with or without 1.6 muM NNGH, which was used as an MMP-3 inhibitor. Four independent experiments were performed. Bars represent +/-SD. ( h ) Representative confocal images of MCF-10A cells in a 3D culture treated with 100 ng/ml GST-IL-1beta (second row) or E2 (32 nM, third row) or control (first row) for 7 days to examine the basement membrane via immunofluorescenc {TM}

Immunogen image

Immunogen Image IRAK1 Polyclonal Antibody

Product Details

PA5-19855

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

10 µg/mL

Immunoprecipitation (IP)

2-4 µg

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A peptide corresponding to amino acids near the carboxy terminus of human IRAK.

3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

100 µg

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_11152551

Product Specific Information

A suggested positive control is Hela cell lysate.

PA5-19855 can be used with blocking peptide PEP-0001.

Target Information

IL-1 receptor associated kinases (IRAKs) are serine/threonine kinases involved in signaling via Toll/IL-1 pathways. It is rapidly recruited by MYD88 to the receptor-signaling complex upon TLR activation. IRAK's association with MYD88 leads to IRAK1 phosphorylation by IRAK4 and subsequent autophosphorylation and kinase activation. Alternatively, IRAK1 can phosphorylate TIRAP to promote its ubiquitination and subsequent degradation. IRAK1 also phosphorylates the interferon regulatory factor 7 (IRF7) to induce its activation and translocation to the nucleus. This results in the transcriptional activiation of type I IFN genes, which drive the cell in an antiviral state.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: hIRAK1c; protein kinase; inactive protein kinase; signaling molecule; Interleukin-1 receptor-associated kinase 1; interleukin-1 receptor-associated kinase-1; IRAK; IRAK-1; mPLK; Pelle homolog; Pelle-like protein kinase

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Gene Aliases: AA408924; Il1rak; IRAK; IRAK-1; IRAK1; IRAK1-S; mPLK; pelle; Plpk; RGD1563841

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UniProt ID: (Human) P51617, (Mouse) Q62406

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Entrez Gene ID: (Human) 3654, (Mouse) 16179, (Rat) 363520

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Function(s)

nucleotide binding protein kinase activity protein serine/threonine kinase activity protein binding ATP binding kinase activity transferase activity protein kinase binding heat shock protein binding identical protein binding protein homodimerization activity protein heterodimerization activity protein serine kinase activity interleukin-1 receptor binding transferase activity, transferring phosphorus-containing groups

Process(es)

regulation of cytokine-mediated signaling pathway toll-like receptor signaling pathway immune system process MyD88-dependent toll-like receptor signaling pathway regulation of transcription, DNA-templated signal transduction I-kappaB kinase/NF-kappaB signaling JNK cascade Toll signaling pathway positive regulation of macromolecule metabolic process cytokine-mediated signaling pathway lipopolysaccharide-mediated signaling pathway positive regulation of type I interferon production response to lipopolysaccharide toll-like receptor 2 signaling pathway toll-like receptor 4 signaling pathway toll-like receptor 9 signaling pathway cellular response to heat intracellular signal transduction interleukin-33-mediated signaling pathway positive regulation of I-kappaB kinase/NF-kappaB signaling negative regulation of I-kappaB kinase/NF-kappaB signaling innate immune response protein autophosphorylation positive regulation of smooth muscle cell proliferation positive regulation of NF-kappaB transcription factor activity type I interferon signaling pathway interleukin-1-mediated signaling pathway response to interleukin-1 cellular response to lipopolysaccharide cellular response to hypoxia positive regulation of intracellular signal transduction positive regulation of leukocyte adhesion to vascular endothelial cell protein phosphorylation activation of NF-kappaB-inducing kinase activity phosphorylation negative regulation of NF-kappaB transcription factor activity response to peptidoglycan positive regulation of MAP kinase activity positive regulation of JUN kinase activity negative regulation of transcription, DNA-templated protein oligomerization negative regulation of cholesterol efflux aging

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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IRAK1 Polyclonal Antibody

IRAK1 Antibody (PA5-19855) in WB

Product Details

PA5-19855

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Amount

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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