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Invitrogen
Dynactin 1 Polyclonal Antibody
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FIGURE: 1 / 11
Dynactin 1 Antibody (PA5-21289) in ICC/IF
Immunofluorescence analysis of Dynactin 1 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Dynactin 1 Polyclonal Antibody (Product # PA5-21289) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsiu... View More
Product Details
PA5-21289
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Non-human primate,
Rat
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human DCTN1. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 20% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recommended positive controls: U87-MG, SK-N-SH, IMR32, SK-N-AS, Jurkat, Raji, NCI-H929, mouse brain, rat brain.
Predicted reactivity: Mouse (100%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
This gene encodes the largest subunit of dynactin, a macromolecular complex consisting of 10 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. Dynactin is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit interacts with dynein intermediate chain by its domains directly binding to dynein and binds to microtubules via a highly conserved glycine-rich cytoskeleton-associated protein (CAP-Gly) domain in its N-terminus. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. Mutations in this gene cause distal hereditary motor neuronopathy type VIIB (HMN7B) which is also known as distal spinal and bulbar muscular atrophy (dSBMA).
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 150 kDa dynein-associated polypeptide; DAP 150; DAP-150; DAP150; DCTN 1; DP 150; DP-150; DP150; dynactin 1 (p150, glued homolog, Drosophila); dynactin 1, retrograde axonal transport; Dynactin subunit 1; Dynactin1; HMN7B; OTTHUMP00000202491; p135; p150 glued; p150 glued homolog; p150-glued; p150glued; unnamed protein product
Gene Aliases:
AL022633; DAP-150; DCTN1; DP-150; Glued; HMND14; P135; p150; p150
UniProt ID: (Human) Q14203, (Rat) P28023, (Mouse) O08788
Entrez Gene ID: (Human) 1639, (Rat) 29167, (Mouse) 13191
establishment of mitotic spindle orientation
mitotic cell cycle
nuclear migration
nervous system development
neuromuscular junction development
centriole-centriole cohesion
ventral spinal cord development
positive regulation of microtubule polymerization
melanosome transport
microtubule anchoring at centrosome
retrograde transport, endosome to Golgi
neuromuscular process
nuclear envelope disassembly
cell division
regulation of mitotic spindle organization
motor behavior
neuron cellular homeostasis
positive regulation of microtubule nucleation
axonal transport
maintenance of synapse structure
positive regulation of neuromuscular junction development
non-motile cilium assembly
neuron projection maintenance
microtubule-based process
metabolic process
transport along microtubule
cytoplasmic microtubule organization
positive regulation of intracellular protein transport
transport
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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