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Invitrogen
DUT Polyclonal Antibody
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FIGURE: 1 / 3
DUT Antibody (PA5-79175) in IHC (P)
Immunohistochemical analysis of DUT in paraffin-embedded section of rat heart tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL rabbit anti-DUT antibody (Product # PA5-79175) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as... View More
Product Details
PA5-79175
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human DUT (212-229aa KKGDRIAQLICERIFYPE).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.05mg sodium azide, 0.05mg thimerosal
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: HELA whole cell, SMMC-7721 whole cell. IHC: human intestinal cancer tissue, rat heart tissue.
Target Information
This gene encodes an essential enzyme of nucleotide metabolism. The encoded protein forms a ubiquitous, homotetrameric enzyme that hydrolyzes dUTP to dUMP and pyrophosphate. This reaction serves two cellular purposes: providing a precursor (dUMP) for the synthesis of thymine nucleotides needed for DNA replication, and limiting intracellular pools of dUTP. Elevated levels of dUTP lead to increased incorporation of uracil into DNA, which induces extensive excision repair mediated by uracil glycosylase. This repair process, resulting in the removal and reincorporation of dUTP, is self-defeating and leads to DNA fragmentation and cell death. Alternative splicing of this gene leads to different isoforms that localize to either the mitochondrion or nucleus. A related pseudogene is located on chromosome 19.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Deoxyuridine 5'-triphosphate nucleotidohydrolase; Deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial; DUT; dUTP diphosphatase; dUTP nucleotidohydrolase; dUTP pyrophosphatase; dUTPase; FLJ20622; PIP4; PPAR-interacting protein 4; unnamed protein product
Gene Aliases: 5031412I06Rik; 5133400F09Rik; BMFDMS; D2Bwg0749e; DUT; Dutp; dUTPase; PIP4
UniProt ID: (Human) P33316, (Rat) P70583, (Mouse) Q9CQ43
Entrez Gene ID: (Human) 1854, (Rat) 497778, (Mouse) 110074
magnesium ion binding
RNA binding
dUTP diphosphatase activity
protein binding
hydrolase activity
receptor inhibitor activity
pyrimidine deoxyribonucleotide binding
identical protein binding
peroxisome proliferator activated receptor binding
poly(A) RNA binding
hydrolase
phosphatase
metabolite interconversion enzyme
liver development
nucleobase-containing compound metabolic process
dUMP biosynthetic process
dTMP biosynthetic process
DNA replication
nucleotide metabolic process
pyrimidine deoxyribonucleoside monophosphate biosynthetic process
pyrimidine deoxyribonucleotide biosynthetic process
regulation of protein complex assembly
dUTP catabolic process
response to organic cyclic compound
regulation of protein heterodimerization activity
protein homotrimerization
negative regulation of receptor activity
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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