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Invitrogen
CD43 Polyclonal Antibody
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FIGURE: 1 / 4
CD43 Antibody (PA5-87221) in IHC (P)
Immunohistochemistry analysis of CD43 in paraffin-embedded mouse spleen. Samples were incubated with CD43 polyclonal antibody (Product # PA5-87221) using a dilution of 1:100. Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Product Details
PA5-87221
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant full length Human SPN.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Purity is > 95% by SDS-PAGE.
Target Information
CD43, also known as leukosialin or sialophorin, is a transmembrane mucin-like protein characterized by a high negative charge. It is expressed on the surface of most hematopoietic cells, excluding resting B lymphocytes. CD43 serves both adhesive and anti-adhesive functions, contributing to a repulsive barrier that can interfere with cellular adhesion while also promoting leukocyte aggregation in certain contexts. CD43 is involved in the activation of T cells, B cells, NK cells, and monocytes. Its expression decreases upon activation due to proteolytic processing. The counter-receptor for CD43 is CD169/SIGLEC-1, which is expressed on macrophages. CD43 interacts with actin-binding proteins ezrin and moesin, playing a regulatory role in remodeling T-cell morphology and regulating cell-cell interactions during lymphocyte traffic. It enhances LFA-1 adhesiveness while counteracting LFA-1 induction via other receptors. CD43 signaling induces the functionally active tumor suppressor p53 protein, but in cases of p53 and ARF deficiency, it can promote tumor proliferation and viability. CD43 is an important modulator of leukocyte functions, with dysfunctions associated with diseases such as Wiscott-Aldrich Syndrome and Adenoid Basal Cell Carcinoma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 3E8 antigen; B-cell differentiation antigen LP-3; CD43; Galactoglycoprotein; GALGP; GPL115; Leukocyte sialoglycoprotein; Leukosialin; leukosianin; Ly-48; Lymphocyte antigen 48; Sialophorin; sialophorin (gpL115, leukosialin, CD43); sialophorin gpL115; sialophorin precursor; unnamed protein product; W3/13 antigen
Gene Aliases: A630014B01Rik; CD43; GALGP; GPL115; LEU-22; LSN; Lsn1; Ly-48; Ly48; SPN
UniProt ID: (Human) P16150, (Mouse) P15702
Entrez Gene ID: (Human) 6693, (Rat) 24796, (Mouse) 20737
response to protozoan
negative regulation of type IV hypersensitivity
T-helper 1 cell lineage commitment
chemotaxis
immune response
cellular defense response
negative regulation of cell adhesion
establishment or maintenance of cell polarity
signal transduction
cell surface receptor signaling pathway
T cell costimulation
positive regulation of tumor necrosis factor production
T cell proliferation
positive regulation of T cell proliferation
negative regulation of T cell proliferation
defense response to bacterium
negative thymic T cell selection
regulation of defense response to virus
regulation of immune response
regulation of T cell activation
negative regulation of T cell activation
leukocyte tethering or rolling
apoptotic signaling pathway
regulation of T cell migration
positive regulation of T cell migration
positive regulation of protein phosphorylation
chronic inflammatory response
monocyte activation
positive regulation of tumor necrosis factor biosynthetic process
thymocyte aggregation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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