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Invitrogen
Human CDK4/cyclin D3, GST Tag Recombinant Protein
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Product Details
A33736
Product Specifications
Species
Human
Expression System
Insect cells
Amino acid sequence
CDK4: 1-303, Cyclin D3: 1-292
Tag
GST-tag
Molecular weight
60.0-58.8 kDa
Class
Recombinant
Type
Protein
Conjugate
Form
Liquid
Concentration
See Label
Purification
purified
Storage buffer
50mM tris HCl, pH 7.5, with 25% glycerol, 0.1mM PMSF, 150mM NaCl, 0.1mM EDTA, 0.25mM DTT, 10mM glutathione
Contains
no preservative
Storage conditions
-80°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Dry ice
Product Specific Information
For maximum recovery please spin prior to use. Unless noted below, aliquots of the 5 µg, 10 µg and 20 µg sizes of kinase are not recommended as materials can be used in original packaging until exhausted. For larger sizes, the number of freeze/thaws may be reduced by preparing aliquots, aliquots below 20 µL are not recommended. Please never store a kinase diluted. If properly stored at -80C, this product is guaranteed for 6 months from date of purchase.
This CDK4/cyclin D3 recombinant human protein (CDK4: full length, cyclin D3: full length) was expressed in insect cells.
CDK4/cyclin D3 recombinant human protein is also available in our SelectScreen™ Kinase Profiling Service.
• Validated for use with the Adapta™ assay
• Available in multiple pack sizes for convenient use
• Also available in our SelectScreen Kinase Profiling Service
Protein Form: Full Length, Recombinant, Active
The gene accession number for the subunits CKD4 and Cyclin D3 are NP_000066.1 and NP_001751.1.
Target Information
CDK4, also known as Cyclin-Dependent Kinase 4, is a member of the cyclin-dependent kinase (CDK) family. These kinases are key regulators of cell cycle progression. CDK4 is particularly involved in driving the transition from the G1 phase to the S phase of the cell cycle.
Cyclin D3 is a regulatory subunit that binds to CDK4 and activates its kinase activity. The binding of Cyclin D3 to CDK4 is essential for the proper functioning of CDK4, as cyclins regulate the activation and specificity of CDKs.
Together, the CDK4/Cyclin D3 complex plays a crucial role in promoting cell cycle progression, specifically by phosphorylating the retinoblastoma protein (Rb), which leads to the release of E2F transcription factors and the subsequent activation of genes required for DNA synthesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Cell division protein kinase 4; Cyclin-dependent kinase 4; D3-type cyclin; G1/S-specific cyclin-D3; PSK-J3
Gene Aliases: CCND3; CDK4; CMM3; PSK-J3
UniProt ID: (Human) P30281, (Human) P11802
Entrez Gene ID: (Human) 896, (Human) 1019
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