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Gibco
Human IL-17A Recombinant Protein
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Product Details
PHC9171
Product Specifications
Species
Human
Expression System
E. coli
Amino acid sequence
Human IL-17A recombinant protein is homodimeric containing two 137 amino acid subunits
Molecular weight
15.5 kDa
Class
Recombinant
Type
Protein
Purity
>95% by SDS-PAGE
Endotoxin concentration
<0.1 ng/µg
Activity
ED50 = 0.5 - 5.0 ng/mL; determined by the dose-dependent production of GRO-alpha by HT-29 cells.
Conjugate
Form
Lyophilized
Purification
purified
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Wet ice (domestic); Wet or Dry ice (international)
Product Specific Information
Carrier-Free
Reconstitution: We recommend that the vial be briefly centrifuged prior to opening to bring the contents to the bottom. Lyophilized human IL-17A should be reconstituted in sterile, distilled water to a concentration of 0.1-1.0 mg/mL. For maximal stability, a carrier protein, such as 0.1-1.0% BSA, may be included. The reconstituted protein should be apportioned into working aliquots and stored at ≤-20°C. Further dilutions should be made in low endotoxin medium or buffered solution with FBS or tissue culture grade BSA.
Storage: For maximal stability, store this lyophilized preparation at ≤-20°C, preferably desiccated. For shipment and short term storage (up to 2 weeks), storage at 2°C to 8°C is sufficient. Reconstituted human IL-17A should be stored at ≤-20°C (not in a frost-free freezer). Keep freeze-thaw cycles to a minimum.
Target Information
Interleukin-17A (IL-17A, CTLA-8) is a CD4+ T cell-derived cytokine that promotes inflammatory responses in cell lines and is elevated in rheumatoid arthritis, asthma, multiple sclerosis, psoriasis, and transplant rejection. IL-17A is a 32 kDa long, disulfide-linked homodimer consisting of 136 amino acids that is a member of a six-species family of proteins (IL-17A-17F) and signals through the IL-17 receptor (IL-17R/CDw217). High levels of IL-17A homodimer are produced by activated peripheral blood CD4+ T-cells, and IL-17A also enhances expression of the intracellular adhesion molecule-1 (ICAM-1) in human fibroblasts. In particular, human IL-17A also stimulates epithelial, endothelial, or fibroblastic cells to secrete IL-6, IL-8, G-CSF, and PGE2. In the presence of human IL-17A, fibroblasts can sustain the proliferation of CD34+ hematopoietic progenitors and induce maturation into neutrophils. Mouse, rat, and human IL-17A can induce IL-6 secretion in mouse stromal cells, indicating that all homologs can recognize the mouse receptor. IL-17A regulates the activities of NF-kappa B and mitogen-activated protein kinases, stimulates the expression of IL-6 and cyclooxygenase-2 (PTGS2/COX-2), and enhances the production of nitric oxide (NO).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CTLA-8; Cytotoxic T-lymphocyte-associated antigen 8; cytotoxic T-lymphocyte-associated protein 8; il 17; IL-17; ILN; Interleukin; interleukin 17; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8); Interleukin-17A; Interleukin17A; R-IL-17-A
Gene Aliases: CTLA-8; CTLA8; IL-17; IL-17A; IL17; IL17A
UniProt ID: (Human) Q16552
Entrez Gene ID: (Human) 3605
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