Invitrogen

Human NEK8, GST Tag Recombinant Protein

Product Details

A33385

Applications

Tested Dilution

Publications

Kinase Assay (KA)

Assay-dependent

Product Specifications

Species

Human

Expression System

Insect cells

Amino acid sequence

1-322

Tag

GST-tag

Molecular weight

62.3 kDa

Class

Recombinant

Type

Protein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

See Label

Purification

purified

Storage buffer

50mM tris HCl, pH 7.5, with 10mM glutathione, 0.1mM EDTA, 25% glycerol, 0.1mM PMSF, 150mM NaCl, 0.25mM DTT

Contains

no preservative

Storage conditions

-80°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Dry ice

Product Specific Information

For maximum recovery please spin prior to use. Unless noted below, aliquots of the 5 µg, 10 µg and 20 µg sizes of kinase are not recommended as materials can be used in original packaging until exhausted. For larger sizes, the number of freeze/thaws may be reduced by preparing aliquots, aliquots below 20 µL are not recommended. Please never store a kinase diluted. If properly stored at -80C, this product is guaranteed for 6 months from date of purchase.

This NEK8 recombinant human protein (1-322) was expressed in insect cells.

NEK8 recombinant human protein is also available in our SelectScreen™ Kinase Profiling Service.

• Validated for use with the LanthaScreen™ Eu Kinase Binding assay
• Available in multiple pack sizes for convenient use
• Also available in our SelectScreen Kinase Profiling Service

Protein Form: Recombinant, Active, Catalytic

The gene accession number for NEK8 is NP_835464.1.

Target Information

NEK8 serine/threonine kinase or NIMA-related kinase 8 is related to NIMA (never in mitosis, gene A) of Aspergillus nodulins and may play a role in cell cycle progression from G2 to M phase. NEK8 is part of a complex of ciliary proteins required for renal and cardiovascular development. NEK8 interacts with polycystin signal transduction pathways and may control the targeting of these ciliary proteins. Required for renal tubular integrity. May regulate local cytoskeletal structure in kidney tubule epithelial cells. May regulate ciliary biogenesis through targeting of proteins to the cilia.Defects in NEK8 are the cause of nephronophthisis type 9 (NPHP9) [MIM:613824]. NPHP9 is an autosomal recessive disorder resulting in end-stage renal disease. It is a progressive tubulo-interstitial kidney disorder histologically characterized by modifications of the tubules with thickening of the basement membrane, interstitial fibrosis and, in the advanced stages, medullary cysts.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: NEK 8; nephrocystin 9; Never in mitosis A-related kinase 8; NIMA (never in mitosis gene a)- related kinase 8; NIMA-family kinase NEK8; NIMA-related kinase 12a; NIMA-related kinase 8; Nima-related protein kinase 12a; NimA-related protein kinase 8; Serine/threonine-protein kinase Nek8; serine/thrionine-protein kinase NEK8