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Invitrogen
Human PD-1 His-tag Recombinant Protein
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Product Details
RP-88146
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Expression System
HEK293 cells
Amino acid sequence
Met1-Gln167
Tag
His-tag
Molecular weight
41.1 kDa
Class
Recombinant
Type
Protein
Purity
≥95% as determined by SDS-PAGE. ≥90% as determined by SEC-HPLC.
Activity
1. Immobilized human PD-1 at 2 µg/ml (100 µL/well) can bind PD-L1 Protein, Human, Recombinant (ECD, hFc Tag), the EC50 of human B7-H1 is 150-600 ng/mL. 2. Immobilized human PD-1 at 2 µg/mL (100 µL/well) can bind Anti-PD1 (MDX)-IgG4 Antibody (Nivolumab) ,the EC50 is 8-60 ng/mL. 3. Labeled biotin to PD-1 Protein, Human, Recombinant (His Tag) by a certain molar ratio; Using the Octet RED System, the affinity constant (Kd) of PD-1 Protein, Human, Recombinant (His Tag), Biotinylated (RP-88146-B) bound to Keytruda was 0.1 nM.
Conjugate
Form
Lyophilized
Concentration
0.25 mg/mL
Amount
1 mg
Purification
purified
Storage buffer
50mM MOPS, pH 7, with 500mM NaCl, 15% trehalose
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
It is recommended that the protein be aliquoted for optimal storage.
Reconstituted with sterile deionized water to 0.25 mg/mL.
Concentration is measured by UV.
Target Information
Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antig en-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PDL-1 and PDL-2. Upon binding to either of these ligands, signals generated by PD-1 inhibit the activation of the immune response in the absence of "danger signals" such as LPS or other molecules associated with bacteria or other pathogens. Evidence for this is seen in PD1-null mice who exhibit hyperactivated immune systems and autoimmune diseases. Despite its predicted molecular weight, PD-1 often migrates at higher molecular weight in SDS-PAGE.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD279; CD279; PD-1; hPD1; PD-1; PD1; alternatively spliced; PDCD1; programmed cell death 1 protein; Programmed cell death protein 1; Protein PD-1; Protein PD1; sCD279; soluble CD279; systemic lupus erythematosus susceptibility 2; unnamed protein product
Gene Aliases: AIMTBS; CD279; hPD-1; hPD-l; hSLE1; PD-1; PD1; PDCD1; SLEB2
UniProt ID: (Human) Q15116
Entrez Gene ID: (Human) 5133
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