Invitrogen

Human PIK3CA/PIK3R3 (p110 alpha/p55 gamma), His Tag Recombinant Protein

Datasheet

Tech Support

Datasheet

Tech Support

Product Details

A33393

Applications

Tested Dilution

Publications

Kinase Assay (KA)

Assay-dependent

Product Specifications

Species

Human

Expression System

Insect cells

Amino acid sequence

Full length

Tag

His-tag

Molecular weight

125.4-56.3 kDa

Class

Recombinant

Type

Protein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

See Label

Purification

purified

Storage buffer

50mM sodium phosphate, pH 7, with 25% glycerol, 0.1mM PMSF, 0.25mM DTT, 300mM NaCl, 150mM Imidazole

Contains

no preservative

Storage conditions

-80°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Dry ice

Product Specific Information

For maximum recovery please spin prior to use. Unless noted below, aliquots of the 5 µg, 10 µg and 20 µg sizes of kinase are not recommended as materials can be used in original packaging until exhausted. For larger sizes, the number of freeze/thaws may be reduced by preparing aliquots, aliquots below 20 µL are not recommended. Please never store a kinase diluted. If properly stored at -80C, this product is guaranteed for 6 months from date of purchase.

This PIK3CA/PIK3R3 (p110 alpha /p55 gamma) recombinant human protein (full length) was expressed in insect cells.

PIK3CA/PIK3R3 (p110 alpha /p55 gamma) recombinant human protein is also available in our SelectScreen™ Kinase Profiling Service.

• Validated for use with the Adapta™ assay
• Available in multiple pack sizes for convenient use
• Also available in our SelectScreen Kinase Profiling Service

Protein Form: Full Length, Recombinant, Active

The gene accession number for PIK3CA is NP_006209.2 and for PIK3R3 is AAH21622.

Target Information

PIK3CA/PIK3R3 lipid kinase (p110 alpha /p55 gamma) or Phosphatidylinositol 3-kinase (PIK3) phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position which serves as second messenger in growth signaling pathways. PIK3CA comprises a 110 kDa catalytic subunit and a regulatory subunit of either 85, 55, or 50 kDa. PIK3CA plays an important role in the metabolic actions of insulin, and a mutation in the PIK3CA has been associated with insulin resistance and plays an important role in glucose homeostasis in vivo. PIK3CA also plays an essential role in the development and induction of mast cells in normal and pathogenic immune responses.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: p55PIK; Phosphatidylinositol 3-kinase 55 kDa regulatory subunit gamma; Phosphatidylinositol 3-kinase regulatory subunit gamma; phosphatidylinositol 3-kinase, catalytic, 110-KD, alpha; phosphatidylinositol 3-kinase, catalytic, alpha polypeptide; phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 3 (p55, gamma); Phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha; Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform; phosphatidylinositol-4,5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha; phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit, alpha isoform; Phosphoinositide 3-kinase alpha; Phosphoinositide-3-kinase catalytic alpha polypeptide; phosphoinositide-3-kinase, catalytic, alpha polypeptide; phosphoinositide-3-kinase, regulatory subunit 3 (gamma); PI3-kinase p110 subunit alpha; PI3-kinase regulatory subunit gamma; PI3-kinase subunit alpha; PI3-kinase subunit p55-gamma; PI3K regulatory subunit gamma; PIK3CA; PIK3R3; ptdIns-3-kinase regulatory subunit p55-gamma; PtdIns-3-kinase subunit p110-alpha; Serine/threonine protein kinase PIK3CA

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Gene Aliases: CLOVE; CWS5; MCAP; MCM; MCMTC; p110-alpha; p55; p55-GAMMA; p55PIK; PI3K; PI3K-alpha; PIK3CA; PIK3R3