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Human SCF Protein (PHC2111) in Bioactivity
Immunofluorescence analysis of mast/stem cell growth factor receptor Kit (c-Kit) was performed using HT-29 cells treated with 100 ng/mL of human SCF (c-Kit ligand) recombinant Pprotein (Product # PHC2113) for 30 minutes. Cells labeled with anti-phospho-c-Kit (Tyr703) recombinant polyclonal antibody (22HCLC) (Product # 710762) (Panel a & c; Green). The composite (b & d) represents merged image with DAPI (blue) and F-actin (red) staining. Cells treated with SCF displayed uprgulation of phospho-c-Kit (Tyr703) in comparison with untreated cell... View More
Product Details
PHC2111
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Published species
Human
Expression System
E. coli
Amino acid sequence
EGICRNRVTN NVKDVTKLVA NLPKDYMITL KYVPGMDVLP SHCWISEMVV QLSDSLTDLL DKFSNISEGL SNYSIIDKLV NIVDDLVECV KENSSKDLKK SFKSPEPRLF TPEEFFRIFN RSIDAFKDFV VASETSDCVV SSTLSPEKDS RVSVTKPFML PPVA
Molecular weight
18.54 kDa
Class
Recombinant
Type
Protein
Purity
>95% by SDS-PAGE
Endotoxin concentration
<0.1 ng/µg
Activity
ED50 < 10 ng/mL; determined by the dose-dependent proliferation of human MO-7e cells.
Conjugate
Form
Lyophilized
Purification
purified
Contains
no preservative
Storage conditions
4°C
Shipping conditions
Wet ice
Product Specific Information
Carrier-Free
Reconstitution: Centrifuge the vial briefly, before opening to bring the contents to the bottom. Reconstitute the lyophilized protein in sterile deionized water to 0.1-1.0 mg/mL to regain full activity. Make any further dilutions of the reconstituted protein in low endotoxin medium or buffered solution with FBS or tissue culture grade BSA.
Storage: Store the lyophilized protein at 2-8°C, preferably desiccated. Upon reconstitution, apportion into working aliquots and store at ≤-20°C (not in a frost-free freezer). Avoid repeated freeze-thaw cycles
Target Information
Stem cell factor (SCF) is the ligand of the c-Kit oncogene and is expressed by various structural and inflammatory cells in the airways. Binding of SCF by the c-Kit receptor leads to homodimerization of the receptor and the activation of signalling pathways such as PI-3, PLC-gamma, Jak/STAT, and MAP kinase pathways. SCF expression leads to the induction of mast cell survival and the expression and release of histamine, pro-inflammatory cytokines and chemokines. The inhibition of the SCF/c-Kit pathway leads to a decrease in histamine levels, mast cell and eosinophil infiltration, IL-4 production and airway hyperresponsiveness, suggesting this pathway may be a useful therapeutic target in inflammatory diseases such as asthma. At least two isoforms of SCF are known to exist.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: c-Kit ligand; DKFZp686F2250; familial progressive hyperpigmentation 2; H-SCF; Kit ligand; kit-ligand; M-SCF; Mast cell growth factor; MGF; SCF; steel factor; Stem cell factor
Gene Aliases: DCUA; DFNA69; FPH2; FPHH; Kitl; KITLG; KL-1; MGF; SCF; SF; SHEP7
UniProt ID: (Human) P21583
Entrez Gene ID: (Human) 4254
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