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Product Details
A33513
Product Specifications
Species
Human
Expression System
Insect cells
Amino acid sequence
171-529
Tag
His-tag
Molecular weight
47 kDa
Class
Recombinant
Type
Protein
Conjugate
Form
Liquid
Concentration
See Label
Purification
purified
Storage buffer
50mM HEPES, pH 7.5, with 5mM DTT, 20% glycerol, 100mM NaCl
Contains
no preservative
Storage conditions
-80° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Dry ice
Product Specific Information
For maximum recovery please spin prior to use. Unless noted below, aliquots of the 5 µg, 10 µg and 20 µg sizes of kinase are not recommended as materials can be used in original packaging until exhausted. For larger sizes, the number of freeze/thaws may be reduced by preparing aliquots, aliquots below 20 µL are not recommended. Please never store a kinase diluted. If properly stored at -80C, this product is guaranteed for 6 months from date of purchase.^M
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This WNK1 recombinant human protein (171-529) was expressed in insect cells.^M
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WNK1 recombinant human protein is also available in our SelectScreen™ Kinase Profiling Service.^M
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• Validated for use with the LanthaScreen™ Eu Kinase Binding assay
• Available in multiple pack sizes for convenient use
• Also available in our SelectScreen Kinase Profiling Service^M
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Protein Form: Recombinant, Active, Catalytic^M
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The gene accession number for WNK1 is NP_061852.3.
Target Information
WNK1 serine/threonine kinase is a member of the WNK subfamily. WNK1 may be a key regulator of blood pressure by controlling the transport of sodium and chloride ions. Mutations in this gene have been associated with pseudohypoaldosteronism type II and hereditary sensory neuropathy type II. WNK plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival, and proliferation. Also, acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively. Activates SCNN1A, SCNN1B, SCNN1D, and SGK1. WNK localizes to a segment of kidney involved in salt, potassium, and pH homeostasis, the distal nephron. Mutations in the WNK gene result in Pseudohypaldosteronism and Neuropathy.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Erythrocyte 65 kDa protein; hWNK1; Kinase deficient protein; MGC163339; MGC163341; p65; prostate-derived sterile 20-like kinase; Protein kinase lysine-deficient 1; Protein kinase with no lysine 1; protein phosphatase 1, regulatory subunit 167; Serine/threonine-protein kinase WNK1; serine/threonine-protein kinase WNK1 1; serine/threonine-protein kinase WNK1 2; With no K; WNK lysine deficient protein kinase 1 isoform
Gene Aliases: HSAN2; HSN2; KDP; KIAA0344; p65; PPP1R167; PRKWNK1; PSK; WNK1
UniProt ID: (Human) O15052
Entrez Gene ID: (Human) 65125
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