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Product Details
PMG0075
Product Specifications
Species
Mouse
Expression System
E. coli
Amino acid sequence
GPETLCGAEL VDALQFVCGP RGFYFNKPTG YGSSIRRAPQ TGIVDECCFR SCDLRRLEMY CAPLKPTKAA
Molecular weight
7.6 kDa
Class
Recombinant
Type
Protein
Purity
>98% by SDS-PAGE
Endotoxin concentration
<0.1 ng/µg
Activity
ED50 ≤ 2 ng/mL; determined by the dose-dependent proliferation of FDC-P1 cells.
Conjugate
Form
Lyophilized
Purification
purified
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Wet ice (domestic); Wet or Dry ice (international)
Product Specific Information
Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute IGF-1 in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. This solution can be stored at 2°C to 8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein, such as 0.1% BSA and store in working aliquots at -20°C to -80°C.
Storage: The lyophilized protein is stable at room temperature for 1 month, but should be kept at -20°C for long term storage. Working aliquots stored with a carrier protein are stable for at least 12 months at -20°C to -80°C. Avoid repeated freeze/thaw cycles.
Target Information
IGF1 (Insulin-like growth factor-1) is structurally and functionally related to insulin but has a much higher growth-promoting activity. A variety of cellular responses are induced by IGF1, including cell proliferation, differentiation, migration, and survival. Further, IGF1 is a polypeptide growth factor that stimulates the proliferation of a wide range of cell types in muscle, bone, and cartilage tissue. IGF1 stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regards to enhancing glucose uptake. In circulation, IGFs are predominantly bound to binding proteins (IGFBPs) which prolong the half-life of the IGFs and play a role in delivering them to target tissues. IGF-I is known as one of the most potent activators of the AKT signaling pathway which is known to be a stimulator of proliferation and an inhibitor of programmed cell death. Moreover, mature human IGF-I is 100% homologous with bovine and porcine proteins. Low levels of IGF1 have been linked to Alzheimer's disease. IGF1 is processed from a precursor, bound by a specific receptor, and secreted. Defects in the IGF1 gene are a cause of insulin-like growth factor 1 deficiency and several transcript variants encoding different isoforms have been found.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: H-IGF-1; IGF; IGF-I; IGF-IA; IGF-IB; IGF1A; IGFIa; Insulin like growth factor; Insulin-like growth factor 1; Insulin-like growth factor I; M-IGF-1; OTTHUMP00000195084; R-IGF-1; Somatomedin; Somatomedin C
Gene Aliases: C730016P09Rik; Igf-1; Igf-I; Igf1
UniProt ID: (Mouse) P05017
Entrez Gene ID: (Mouse) 16000
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