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Gibco
PeproGMP® Human IL-3 Recombinant Protein, PeproTech®
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PeproGMP® Human IL-3 Protein (GMP200-03-50UG) in Bioactivity
Bioassay for PeproGMP® Human IL-3 Recombinant Protein, PeproTech (Product # GMP200-03-50UG) compared to a competitors GMP Human IL-3 and Human IL-3, WHO Standard (91/510). Find the International Unit (IU) value here.
Product Details
GMP200-03-50UG
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Published species
Human
Expression System
E. coli
Amino acid sequence
APMTQTTSLK TSWVNCSNMI DEIITHLKQP PLPLLDFNNL NGEDQDILME NNLRRPNLEA FNRAVKSLQN ASAIESILKN LLPCLPLATA APTRHPIHIK DGDWNEFRRK LTFYLKTLEN AQAQQTTLSL AIF
Molecular weight
15 kDa
Class
Recombinant
Type
Protein
Purity
≥ 98% by SDS-PAGE gel and HPLC analyses.
Endotoxin concentration
≤0.1 EU/µg
Activity
Determined by the proliferation of TF-1 cells. PeproGMP Recombinant Human IL-3 has been tested against the WHO standard NIBSC 91/510. Find the International Unit (IU) value here.
Conjugate
Form
Lyophilized
Purification
purified
Contains
no preservative
Storage conditions
-20°C or -80°C if preferred
Shipping conditions
Ambient
Product Specific Information
The IL-3 protein is a hematopoietic growth factor that promotes the survival, differentiation, and proliferation of committed progenitor cells of the megakaryocyte, granulocyte-macrophage, erythroid, eosinophil, basophil and mast cell lineages. The PeproTech Recombinant Human IL-3 is a 15.0 kDa globular protein containing 133 amino acid residues.
PeperoGMP IL-3 cytokine is most often used with PeproGMP SCF, PeproGMP TPO (thrombopoietin) and PeproGMP Flt3-ligand in hematopoietic stem cells (HSCs) cultures. IL-6 may also be used for further synergistic expansion and immune cell maturation.
Reconstitute using sterile water (i.e., WFI) to a concentration of 0.1-1.0 mg/ml.
Additional product information, specifications, handling and reconstitution information are provided on the Certificate of Analysis.
All PeproGMP® products are manufactured using animal-derived component free materials.
Product Description/Intended Use: PeproTech GMP recombinant proteins are manufactured for use as ancillary materials by applying applicable principles of GMP and quality control requirements from USP (United States Pharmacopeia) Chapter <1043> Ancillary Materials for Cell, Gene, and Tissue-Engineered Products.
Target Information
Interleukin 3 (IL-3), also called Mast Cell Growth Factor (MCGF), Multi-Colony Stimulating Factor (multi-CSF), or Eosinophil-CSF (E-CSF), is produced by activated T cells, mast cells and eosinophils. IL-3 is a hematopoietic growth factor that stimulates colony formation of erythroid, megakaryocyte, neutrophil, eosinophil, basophil, mast cell and monocytic lineages. Most of these functions are enhanced or dependent on co-stimulation with other cytokines. Human and mouse IL-3 share only 29% sequence identity at the amino acid level. The bioactivity of IL-3 is highly species specific. For example, human IL-3 has no activity on mouse cells. IL-3 is a potent growth promoting cytokine. IL-3 is capable of supporting the proliferation of a broad range of hematopoietic cell types, and is involved in a variety of cell activities such as cell growth, differentiation and apoptosis. IL-3 has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders.
For research use or further manufacturing. Not for diagnostic use or direct administration into humans or animals.
Bioinformatics
Protein Aliases: BP/BPA; Colony stimulating factor multiple; colony-stimulating factor, multiple; Eo-CSF; Hematopoietic growth factor; IL-3; ILN; Interleukin; Interleukin 3 (colony stimulating factor; Interleukin-3; Interleukin3; Mast cell growth factor; mast-cell growth factor; MCGF; MGC79398; MGC79399; MGF; Multi CSF; Multilineage colony stimulating factor; multilineage-colony-stimulating factor; multiple); Multipotential colony-stimulating factor; P cell stimulating factor; P-cell stimulating factor; P-cell-stimulating factor; PCSA
Gene Aliases: IL-3; IL3; MCGF; MULTI-CSF
UniProt ID: (Human) P08700
Entrez Gene ID: (Human) 3562
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