Shop All Single-Use BioProcessing Systems

HyPerforma™ Mixtainer™ Hardware, Magnetic Stirring Plate; EU 230V, 50/60 Hz,0.5 amps (Thermo Scientific™)

The Thermo Scientific™ Magnetic Stirring Plate (EU 230V, 50/60 Hz,0.5 amps) is part of the Thermo Scientific™ HyPerforma™ Mixtainer™ system to support bioproduction mixing applications. Additional components of the system include the Thermo Scientific™ HyPerforma™ Mixtainer™ BioProcess Container (BPC) with an integrated magnetic stir bar, a conical drum, a support plate and a dolly. The optimized support hardware allows mixing, moving, storage, shipping and discharge of the BPC contents while maintaining sterility of the contents. Standard products are available for sterile liquid-to-liquid or powder-to-liquid mixing.

Recommended for:
• Hydration of powdered media and buffers
• Sterile mixing of cell culture media with sera and reagents
• Sterile mixing of buffer solutions or other process fluids
• Pooling of sterile liquid fractions

Thermo Scientific™ 5:1 Single-Use Bioreactor, jacketed, AC motor, load cells w/o display, 2000 L (Thermo Scientific™)

This 2000 L Thermo Scientific 5:1 Single-Use Bioreactor (SUB) is water-jacketed and includes AC motor and load cells without display. It is designed using traditional stainless-steel bioreactor principles to ensure optimal cell culture performance. The SUB is a fully functional bioreactor vessel including single-use sterile contact surfaces for mixing, venting, sparging, and temperature sensing. The complete line of SUBs include 50, 100, 250, 500, 1000, and 2000-L sizes that ensure consistent scalability from pilot-scale studies, pre-clinical to commercial production.

Features:
• Unique proprietary mixing system that enables conventional overhead mixing while maintaining sterility and integrity of the SUB BioProcess Container (BPC)
• Largest qualified standard component library that allows for customization of SUB BPCs
• Minimized vessel footprint and reduced hold-up volumes
• Offered with AC or DC motors designed for precise agitation control
• Optional cable management tree provides line set and cabling organization for ease of use
• Load cells offered as standard feature with analog or digital communication options
• Option to use industry-leading single-use and traditional sensors for pH, DO, and pressure monitoring
• Option to choose the bioreactor vessel as a stand-alone system or complete turnkey system with dedicated controls from either the Finesse™ or Applikon™ families of control systems for bioproduction. These systems work on PC, Delta V™, Allen-Bradley™, or Siemens™ software platforms
• Updated 5:1 mixing turn-down ratio in combination with patented cross-flow sparger technology introduces gas just above liquid’s surface to facilitate efficient mass transfer CO2 stripping and reduce bubble sparging and associated foam damage to cells. This feature allows customers to do more with fewer vessels, which improves productivity and reduces costs simultaneously.

Applications of the Thermo Scientific SUB include:
• Batch, fed batch, and perfusion cultures
• Suspension and microcarrier cultures
• Proven for various cell lines such as CHO, Sp2/0 hybridoma, PER.C6, HEK293, Vero, MDCK, etc.

Accessories:
• Tube Assembly, Cat # SH3072002
• Molded Probe Holder, Cat # SV50177P01
• HARDWARE: Vent Filter Heater; Bulb Controller, Cat# SV5019134
• HARDWARE: Drive Shaft; Quick Connect 2 piece; Long Tip, Cat# SV5095921

HyPerforma™ Mixtainer™ Hardware, Stainless-Steel Support Plate; EU (Thermo Scientific™)

The Thermo Scientific™ Stain-less Steel Support Plate (EU) is part of the Thermo Scientific™ HyPerforma™ Mixtainer™ system to support bioproduction mixing applications. Additional components of the system include the Thermo Scientific™ HyPerforma™ Mixtainer™ BioProcess Container (BPC) with an integrated magnetic stir bar, a conical drum, a magnetic stirring plate and a dolly. The optimized support hardware allows mixing, moving, storage, shipping and discharge of the BPC contents while maintaining sterility of the contents. Standard products are available for sterile liquid-to-liquid or powder-to-liquid mixing.

Recommended for:
• Hydration of powdered media and buffers
• Sterile mixing of cell culture media with sera and reagents
• Sterile mixing of buffer solutions or other process fluids
• Pooling of sterile liquid fractions

HyPerforma™ G3Lite Controller, for 100 L S.U.B. (GMP) (Thermo Scientific™)

The Thermo Scientific HyPerforma G3Lite Controller is an open architecture control system that can be integrated with any single-use bioreactor, such as the HyPerforma Single-Use Bioreactor (S.U.B.) (available in sizes ranging from 50 L to 2,000 L). The system consists of a control tower that leverages intelligent transmitters, mass flow controllers (MFCs), pumps, and sensors, and TruBio software that facilitates easy, reliable, and repeatable process development and commercial cell culture processes. HyPerforma G3Lite Controllers are fully self-contained, movable units that can be operated as a stand-alone unit (for one vessel) or networked for multiple vessels. They are engineered to optimize capital cost for use in non-GMP and cGMP-certified production facilities.

HyPerforma G3Lite Controller features include:
• Open architecture capabilities to integrate with vessels from other suppliers (Sartorius, Eppendorf, Millipore, and Applikon)
• A common control platform throughout a product’s life cycle
• Flexible upstream TruBio software powered by the Emerson™ DeltaV™ system
• Nonproprietary, industry-standard, cGMP-validated (requires validation package) distributed control system (DCS) that provides networked multiple-vessel control capability

Applications
• Suspension and adherent cell culture processes
• Commercial manufacturing workflows

HyPerforma™ Process Development Automation Package, for 4 G3Lab Controller Systems (Thermo Scientific™)

The Thermo Scientific HyPerforma Automation Packages are specifically designed for PD scale bioprocessing applications. The system features Thermo Scientific TruBio software that facilitates easy, reliable, and repeatable process development cell culture processes.

HyPerforma PD Automation Package features include:
• Open architecture capabilities to integrate with vessels from other suppliers (Sartorius, Eppendorf, Millipore,and Applikon)
• The ability to build and manage complex, multi-feed dosing strategies that provide for third-party peripheral integration as needed

Applications
• Suspension and adherent cell culture processes
• Fermentation processes
• PD scale to full cGMP manufacturing workflows

Thermo Scientific™ 5:1 Single-Use Bioreactor, jacketed, AC motor, 240VAC, e-box, analog load cells, 2000 L (Thermo Scientific™)

This 2000 L Thermo Scientific 5:1 Single-Use Bioreactor (SUB) is water-jacketed and includes AC motor, 240VAC, e-box, and analog load cells. It is designed using traditional stainless-steel bioreactor principles to ensure optimal cell culture performance. The SUB is a fully functional bioreactor vessel including single-use sterile contact surfaces for mixing, venting, sparging, and temperature sensing. The complete line of SUBs include 50, 100, 250, 500, 1000, and 2000-L sizes that ensure consistent scalability from pilot-scale studies, pre-clinical to commercial production.

Features:
• Unique proprietary mixing system that enables conventional overhead mixing while maintaining sterility and integrity of the SUB BioProcess Container (BPC)
• Largest qualified standard component library that allows for customization of SUB BPCs
• Minimized vessel footprint and reduced hold-up volumes
• Offered with AC or DC motors designed for precise agitation control
• Optional cable management tree provides line set and cabling organization for ease of use
• Load cells offered as standard feature with analog or digital communication options
• Option to use industry-leading single-use and traditional sensors for pH, DO, and pressure monitoring
• Option to choose the bioreactor vessel as a stand-alone system or complete turnkey system with dedicated controls from either the Finesse™ or Applikon™ families of control systems for bioproduction. These systems work on PC, Delta V™, Allen-Bradley™, or Siemens™ software platforms
• Updated 5:1 mixing turn-down ratio in combination with patented cross-flow sparger technology introduces gas just above liquid’s surface to facilitate efficient mass transfer CO2 stripping and reduce bubble sparging and associated foam damage to cells. This feature allows customers to do more with fewer vessels, which improves productivity and reduces costs simultaneously.

Applications of the Thermo Scientific SUB include:
• Batch, fed batch, and perfusion cultures
• Suspension and microcarrier cultures
• Proven for various cell lines such as CHO, Sp2/0 hybridoma, PER.C6, HEK293, Vero, MDCK, etc.

Accessories:
• Tube Assembly, Cat # SH3072002
• Molded Probe Holder, Cat # SV50177P01
• HARDWARE: Vent Filter Heater; Bulb Controller, Cat# SV5019134
• HARDWARE: Drive Shaft; Quick Connect 2 piece; Long Tip, Cat# SV5095921

inSITE™ Integrity Testing System, single-channel, Europe (Thermo Scientific™)

The Thermo Scientific™ inSITE™ Integrity Testing System is a single-channel (Europe), AC-powered quality assurance testing system. The inSITE system uses pressure decay measurements to detect leaks that can occur between the warehouse and the clean room floor, or from improper handling and loading procedures. The system can also be used to manage overall quality of bioprocess containers up to the liquid filling cycle.

Features
• Inflation cycle—assists users in loading Thermo Scientific™ BioProcess Containers (BPCs) with minimal interaction, helping to reduce the risk of handling errors
• Gross leak detection—finds small leaks (100 μm–1,000 μm) and confirms connection and setup of the BPC
• Fine leak detection—unique for each tank and BPC assembly
• Liquid filling cycle—moderates the internal pressure within the BPC while fluid is being introduced
• Inflation port—separate port that allows all other ports to remain closed
• Wireless PC
• Label printer
• Bar code scanner
• USB port

Applications
• Point-of-use integrity testing up to 5,000 L
• Gross and fine leak detection
• Guided validation setup
• Improved loading and placement in tanks
• Internal pressure regulation while fluid is being introduced
• Comparative pressure data analysis

inSITE specifications
• Size: 66 x 74 x 147 cm (26 x 29 x 58 in.)
• Weight: 124.7 kg (275 lbs.)
• Power requirements: 220 VAC, 15A, 50 Hz
• Maximum operating pressure: 25−40 mbar
• Room temperature: 65−75°F (18−24°C)
• Maximum measurable volume: 5,000 L

BioProcess Container (BPC), 100L, for HyPerforma™ Single-Use Mixer, liquid-to-liquid, Aegix5-14 film, 0 ports (Thermo Scientific™)

The 100L Thermo Scientific™ Bioprocess Containers (BPC) for liquid-to-liquid mixing, is composed of Aegis 5-14 single web, multi-layer plastic film and includes 0 ports.

Single Use Mixer (S.U.M.) BPC options include:
• Open top liner for media/buffer hydration
• Closed BPC with powder port for contained media/buffer hydration
• Closed BPC with powder port and monitoring probe capability
• Closed BPC for liquid-liquid mixing of critical sterile solutions such as pooling of fractions or processing of intermediate solutions
• Closed BPC for liquid-liquid mixing with monitoring probe capability

HyPerforma™ Mixtainer™ Hardware, Stainless-Steel Support Plate; US (Thermo Scientific™)

The Thermo Scientific™ Stain-less Steel Support Plate (US) is part of the Thermo Scientific™ HyPerforma™ Mixtainer™ system to support bioproduction mixing applications. Additional components of the system include the Thermo Scientific™ HyPerforma™ Mixtainer™ BioProcess Container (BPC) with an integrated magnetic stir bar, a conical drum, a magnetic stirring plate and a dolly. The optimized support hardware allows mixing, moving, storage, shipping and discharge of the BPC contents while maintaining sterility of the contents. Standard products are available for sterile liquid-to-liquid or powder-to-liquid mixing.

Recommended for:
• Hydration of powdered media and buffers
• Sterile mixing of cell culture media with sera and reagents
• Sterile mixing of buffer solutions or other process fluids
• Pooling of sterile liquid fractions

HyPerforma™ G3Lite Controller, for 250 L S.U.B. (non-GMP) (Thermo Scientific™)

The Thermo Scientific HyPerforma G3Lite Controller is an open architecture control system that can be integrated with any single-use bioreactor, such as the HyPerforma Single-Use Bioreactor (S.U.B.) (available in sizes ranging from 50 L to 2,000 L). The system consists of a control tower that leverages intelligent transmitters, mass flow controllers (MFCs), pumps, and sensors, and TruBio software that facilitates easy, reliable, and repeatable process development and commercial cell culture processes. HyPerforma G3Lite Controllers are fully self-contained, movable units that can be operated as a stand-alone unit (for one vessel) or networked for multiple vessels. They are engineered to optimize capital cost for use in non-GMP and cGMP-certified production facilities.

HyPerforma G3Lite Controller features include:
• Open architecture capabilities to integrate with vessels from other suppliers (Sartorius, Eppendorf, Millipore, and Applikon)
• A common control platform throughout a product’s life cycle
• Flexible upstream TruBio software powered by the Emerson™ DeltaV™ system
• Nonproprietary, industry-standard, cGMP-validated (requires validation package) distributed control system (DCS) that provides networked multiple-vessel control capability

Applications
• Suspension and adherent cell culture processes
• Commercial manufacturing workflows

HyPerforma™ Single-Use Mixer, 1000 L, jacketed, DC motor, touchscreen console, with load cells (Thermo Scientific™)

The Thermo Scientific HyPerforma Single-Use Mixer (S.U.M.) with touchscreen console is designed using proven mixer platform principles and used for upstream and downstream mixing applications. The HyPerforma S.U.M. offers an ergonomic and efficient user experience. The complete line of HyPerforma single-use mixers includes 50, 100, 200, 500, 1000 and 2000 L sizes, helping ensure consistent scalability for a number of applications from powder media and buffer hydration to advanced downstream harvest and intermediate pooling steps. The touchscreen console provides an unsurpassed user experience while providing agitation, pump, and TCU control, as well as easy-to-monitor pH, conductivity, temperature, mass, and pressure measurements. The touchscreen console also provides the capability for automated acid, base, and saline titration and automated vessel fill and harvest.

HyPerforma S.U.M. features include:
• Unique proprietary mixing system that provides conventional overhead mixing while maintaining sterility and integrity of the S.U.M. BioProcess Container (BPC)
• Modular graphical touchscreen user interface allows easy viewing of mass, pH, conductivity, temperature, and pressure measurements, and agitation, pump control, and temperature settings (if applicable, for jacketed vessel)
• Data management through Modbus, Profibus, Ethernet, and USB
• Automated acid, base, and saline titration
• Automated mixer fill and harvest control
• Recipe development and storage capabilities
• Simple bag loading with a vertical access door (available on 500 L, 1000 L, and 2000 L sizes)
• Minimized vessel footprint and reduced hold-up volume
• Cable management system provides greater organization for electrical cables, tubing, and line sets
• Dedicated adjustable tool holder keeps necessary tools available
• Mixer vessel is offered non-jacketed (no heat transfer) or with a water jacket (vessel side and bottom). Water jacket is used with separate external temperature control unit for heat transfer requirement.
• Three load cell weighing systems enable accurate batch weight monitoring
• Option to use probes for temperature, pH, conductivity, and pressure monitoring
• S.U.M. BPCs offered in CX5-14 and Aegis5-14 films

Applications of the HyPerforma S.U.M. include:
• Powder media hydration
• Liquid media concentrate dilution
• Harvest hold tank
• Viral inactivation
• Downstream intermediate pooling
• Final product hold and additive suspension

imPULSE™ Single-Use Mixer (S.U.M.) (Thermo Scientific™)

The 500 L Thermo Scientific™ imPULSE™ Single-Use Mixer (S.U.M.) was designed with proven engineered mixer principles and can be used for upstream and downstream mixing applications. The linear-scale design delivers uniform, superior mixing in every model, from 30 L to 5000 L, and mixes to empty. The mixer provides consistent scalability and can be used in numerous applications—from powder media and buffer hydration to advanced downstream harvest and intermediate pooling steps.

The imPULSE Core
The innovative disc design and the hexagonal shape of the disc and vessel are at the core of the quick and thorough mixing capabilities of the imPULSE series. The imPULSE Core line offers superior mixing without high-end controls and monitors. Some basic features and options include:
• Linear mixing scalability from 30 L to 5,000 L
• Hexagonal vessel with open top and sloped bottom with openings for the mixing BPC and tubing outlets
• Integrated control panel with relay logic, manual push buttons, selector switch interface, and digital speed indication
• Stationary powder BPC hanger and lifting system with manual or power winch
• Mixing tank jacket and insulation
• Weight indication system with load cells
• Auto-inflate and vent control options

Applications:
• Media preparation
• Final formulation steps
• Buffer preparation
• Use as a harvest vessel
• Pooling and liquid transfer
• Product suspension
• Mixing and storage of multiple batches
• Viral inactivation

Accessories:
• Tube Assembly, Cat # SH3072002
• Molded Probe Holder, Cat # SV50177P01

Three60™ Sampling System BioProcess Container (BPC) (Thermo Scientific™)

The simple design behind the Thermo Scientific™ Three60™ Sampling System allows you to take a representative sample of your product with minimal effort. The fluid path of the pre-irradiated BioProcess Container (BPC) has a quick-turn valve and pinch-and-cut disconnections for fluid transfer and removal. The 500 mL Three60 Sampling System BPC is composed of Thermo Scientific™ ASI™ 77 film, a 5-layer, 7 mL, polyethylene film and includes 2 ports (1 luer lock insert + cap, 1 injection site).

Features of the system include:
• Compatible with a 3.8 cm (1.5-inch) sanitary connector
• Requires no expensive hardware
• Each Three60 package contains a valve and four assemblies with pinch-and-cut disconnections
• Helps to eliminate post-use cleaning steps required with reusable containers, and to reduce cross-contamination risks

Customize standard BPC systems for optimized single-use technology
Choose from the industry’s largest component library, with over 2,000 unique components that include:
• Fittings—straight, reducing, elbow, T-style, X-style, and cross-style
• Connectors—quick connectors/disconnectors, aseptic connectors and disconnectors, and steam-to and steam-through connectors
• Filters (used for bioburden reduction)—sterilization grade filters and vent filters
• Tubing—thermoplastic elastomers and platinum-cured silicone
• Sample ports—septum-style and needle-free ports
• Clamps—sliders, pinch, and crimp bands

HyPerforma™ G3Lab Controller, for HyPerforma Glass Bioreactor (non-GMP) (Thermo Scientific™)

The Thermo Scientific G3Lab Controller has been specifically designed for PD scale bioprocessing applications. It can control most brands of single-use or autoclavable bioreactors or fermentors with a capacity of ≤50 L, including stirred-tank and rocking models. The controller operates using proprietary Thermo Scientific TruBio software, which provides easy process scale-up/scale-down and the configurability to scale your control strategy along with your process. The enclosure contains state-of-the-art transmitters along with power supplies, pumps, input/output (I/O) modules, and the hardware required to connect to the control network, providing maximum control capability.

G3Lab Controller features include:
• Open architecture capabilities to integrate with vessels from other suppliers (Sartorius, Eppendorf, Millipore, and Applikon)
• A common control platform throughout a product’s life cycle
• Flexible upstream TruBio software powered by the Emerson™ DeltaV™ system
• Nonproprietary, industry-standard, cGMP-validated (with validation package) distributed control system (DCS) that provides networked multiple-vessel control capability

Applications
• Suspension and adherent cell culture processes
• PD scale to full cGMP manufacturing workflows

HyPerforma™ Glass Bioreactor, 240 V, heat only (Thermo Scientific™)

The Thermo Scientific HyPerforma Glass Bioreactor offers easy operation and rapid assembly and is manufactured with the highest standards for materials and surface finish. Developed using a computational fluid dynamics (CFD) simulator, the HyPerforma Glass Bioreactor impellers provide maximum mixing with minimum shear force, resulting in a higher average kLa. The cold finger has been replaced by a cooling loop to increase the thermal surface. Laboratory performance tests have demonstrated a cooling rate two times better than a sample of products from other suppliers. Control can be bidirectional, which allows a single-wall vessel to be used for mammalian applications and fermentation processes.

HyPerforma Glass Bioreactor features include:
• The motor adapter uses coupling windows and an alignment marker for easy assembly
• Ergonomic head plate design provides easy assembly and disassembly of components for rapid reconfiguration

Applications
• Suspension and adherent cell culture processes
• PD scale to full cGMP manufacturing workflows