Shop All Single-Use BioProcessing Systems

Three60™ Sampling System (Thermo Scientific™)

The Thermo Scientific™ Three60™ Sampling System allows you to take a representative sample of your product with minimal effort. The fluid paths of the four pre-irradiated BioProcess Containers (BPCs) and assemblies feature quick-turn valves and pinch-and-cut disconnections for fluid transfer and removal. The 250 mL Three60 Sampling System is composed of Thermo Scientific™ ASI™ 77 film, a 5-layer, 7 mL, polyethylene film and includes 2 ports (1 luer lock insert + cap, 1 injection site).

Features of the system include:
• Compatible with a 3.8 cm (1.5-inch) sanitary connector
• Requires no expensive hardware
• Each Three60 package contains a valve and four assemblies with pinch-and-cut disconnections
• Helps to eliminate post-use cleaning steps required with reusable containers, and to reduce cross-contamination risks

Customize standard BPC systems for optimized single-use technology
Choose from the industry’s largest component library, with over 2,000 unique components that include:
• Fittings—straight, reducing, elbow, T-style, X-style, and cross-style
• Connectors—quick connectors/disconnectors, aseptic connectors and disconnectors, and steam-to and steam-through connectors
• Filters (used for bioburden reduction)—sterilization grade filters and vent filters
• Tubing—thermoplastic elastomers and platinum-cured silicone
• Sample ports—septum-style and needle-free ports
• Clamps—sliders, pinch, and crimp bands

HyPerforma™ Single-Use Fermentor Systems, 30L, Jacketed, AC Motor, with 2 position vent filter bracket and 120 VAC electrical box (Thermo Scientific™)

The 30 L Thermo Scientific™ HyPerforma™ S.U.F., jacketed, AC Motor, with 2 position vent filter bracket and 120VAC electrical box, is designed to provide the users with enhanced functionality, ease of use, and efficiency for microbial fermentation. The HyPerforma S.U.F. system consists of a fermentor tank and Thermo Scientific™ HyPerforma™ S.U.F. BioProcess Container (BPC). The system has a 5:1 turndown ratio, which means it can run at a low 6 L working volume. The HyPerforma S.U.F. maintains traditional stirred-tank fermentation design principles, including specific height-to-diameter ratios (3:1), top-driven impeller location that deliver optimum cell viability, and performance and scalability from process development through production.

Features:
• The S.U.F. is a fully functional fermentor vessel including single-use sterile contact surfaces for mixing, venting, sparging and temperature sensing. Additional ports are included to allow choice of aseptic connections for filling, emptying and sampling.
• Unique proprietary mixing system enables conventional overhead mixing while maintaining sterility and integrity of the S.U.F. BPC.
• Minimized vessel footprint and reduced hold-up volumes.
• Simplified bag loading with a front access door.
• Tank includes baffles that enhance mixing.
• Side and bottom jacket for effective heat transfer.
• Cable management system offers greater organization for electrical cables, tubing and line sets.
• Dedicated adjustable tool holder keeps necessary tools available for convenience.
• BPC utilizes three Rushton-style impellers and sparger configuration with a proprietary exhaust management system.
• State-of-the-art foam sensor that helps to prevent exhaust filter failure.

Applications of the Thermo Scientific HyPerforma S.U.F. include:
• Proven for various microbial cultures of Pichia, Bacillus and others.
• Batch and fed-batch cultures.

Accessory:
Molded Probe Holder, Cat # SV50177P01

imPULSE™ Single-Use Mixer (S.U.M.) (Thermo Scientific™)

The 100 L Thermo Scientific™ imPULSE™ Single-Use Mixer (S.U.M.) was designed with proven engineered mixer principles and can be used for upstream and downstream mixing applications. The linear-scale design delivers uniform, superior mixing in every model, from 30 L to 5000 L, and mixes to empty. The mixer provides consistent scalability and can be used in numerous applications—from powder media and buffer hydration to advanced downstream harvest and intermediate pooling steps.

The imPULSE Core
The innovative disc design and the hexagonal shape of the disc and vessel are at the core of the quick and thorough mixing capabilities of the imPULSE series. The imPULSE Core line offers superior mixing without high-end controls and monitors. Some basic features and options include:
• Linear mixing scalability from 30 L to 5,000 L
• Hexagonal vessel with open top and sloped bottom with openings for the mixing BPC and tubing outlets
• Integrated control panel with relay logic, manual push buttons, selector switch interface, and digital speed indication
• Stationary powder BPC hanger and lifting system with manual or power winch
• Mixing tank jacket and insulation
• Weight indication system with load cells
• Auto-inflate and vent control options

Applications:
• Media preparation
• Final formulation steps
• Buffer preparation
• Use as a harvest vessel
• Pooling and liquid transfer
• Product suspension
• Mixing and storage of multiple batches
• Viral inactivation

Accessories:
• Tube Assembly, Cat # SH3072002
• Molded Probe Holder, Cat # SV50177P01

HyPerforma™ Mixtainer™ Hardware, Conical Drum, 200L (Thermo Scientific™)

The 200L Thermo Scientific™ Conical Drum top dispense, with clamps is utilized as part of the Thermo Scientific™ HyPerforma™ Mixtainer™ system to support bioproduction mixing applications. Additional components of the system include the Thermo Scientific™ HyPerforma™ Mixtainer™ BioProcess Container (BPC) with an integrated magnetic stir bar, a magnetic stirring plate, a support plate and a dolly. The optimized support hardware allows mixing, moving, storage, shipping and discharge of the BPC contents while maintaining sterility of the contents. Standard products are available for sterile liquid-to-liquid or powder-to-liquid mixing.

Recommended for:
• Hydration of powdered media and buffers
• Sterile mixing of cell culture media with sera and reagents
• Sterile mixing of buffer solutions or other process fluids
• Pooling of sterile liquid fractions

HyPerforma™ Smartainer™ Hardware, 200L, Stainless steel outer container (Thermo Scientific™)

The 200L Thermo Scientific™ HyPerforma™ Smartainer is a stainless-steel non-jacketed outer container system designed to accommodate large volume bioprocess containers.

Features:
• Durable containers are constructed from 304 stainless steel
• Ideal for in-house handling applications in critical areas
• Movable and stackable with pallet jack or forklift
• Non-jacketed
• Allow both top and bottom discharge from BPCs

HyPerforma™ Single-Use Fermentor Systems, 30L, Jacketed, AC Motor, with 2 position vent filter bracket and 240 VAC electrical box (Thermo Scientific™)

The 30 L Thermo Scientific™ HyPerforma™ S.U.F., jacketed, AC Motor, with 2 position vent filter bracket and 240VAC electrical box, is designed to provide the users with enhanced functionality, ease of use, and efficiency for microbial fermentation. The HyPerforma S.U.F. system consists of a fermentor tank and Thermo Scientific™ HyPerforma™ S.U.F. BioProcess Container (BPC). The system has a 5:1 turndown ratio, which means it can run at a low 6 L working volume. The HyPerforma S.U.F. maintains traditional stirred-tank fermentation design principles, including specific height-to-diameter ratios (3:1), top-driven impeller location that deliver optimum cell viability, and performance and scalability from process development through production.

Features:
• The S.U.F. is a fully functional fermentor vessel including single-use sterile contact surfaces for mixing, venting, sparging and temperature sensing. Additional ports are included to allow choice of aseptic connections for filling, emptying and sampling.
• Unique proprietary mixing system enables conventional overhead mixing while maintaining sterility and integrity of the S.U.F. BPC.
• Minimized vessel footprint and reduced hold-up volumes.
• Simplified bag loading with a front access door.
• Tank includes baffles that enhance mixing.
• Side and bottom jacket for effective heat transfer.
• Cable management system offers greater organization for electrical cables, tubing and line sets.
• Dedicated adjustable tool holder keeps necessary tools available for convenience.
• BPC utilizes three Rushton-style impellers and sparger configuration with a proprietary exhaust management system.
• State-of-the-art foam sensor that helps to prevent exhaust filter failure.

Applications of the Thermo Scientific HyPerforma S.U.F. include:
• Proven for various microbial cultures of Pichia, Bacillus and others.
• Batch and fed-batch cultures.

Accessory:
Molded Probe Holder, Cat # SV50177P01

BioProcess Container (BPC), 2000L, for HyPerforma™ Single-Use Mixer, powder-to-liquid, Aegis5-14 film, 3 ports (Thermo Scientific™)

The 2000L Thermo Scientific™ Bioprocess Containers (BPC) for powder-to-liquid mixing, is composed of Aegis 5-14 single web, multi-layer plastic film and includes 3 ports.

Single Use Mixer (S.U.M.) BPC options include:
• Open top liner for media/buffer hydration
• Closed BPC with powder port for contained media/buffer hydration
• Closed BPC with powder port and monitoring probe capability
• Closed BPC for liquid-liquid mixing of critical sterile solutions such as pooling of fractions or processing of intermediate solutions
• Closed BPC for liquid-liquid mixing with monitoring probe capability

Sampling manifold accessory for HyPerforma™ Single-Use Bioreactors, Single pack - includes Luer Locks (Thermo Scientific™)

The Sterile Sampling Manifold with Luer Locks (single-pack) is an accessory for the Thermo Scientific™ HyPerforma™ Single-Use Bioreactors and is available to weld onto the sample line to take sample sets.

BioProcess Container (BPC), 100L, for HyPerforma™ Single-Use Mixer, powder-to-liquid, CX5-14 film, 0 ports (Thermo Scientific™)

The 100L Thermo Scientific™ Bioprocess Containers (BPC) for powder-to-liquid mixing, is composed of CX5-14 single web, multi-layer plastic film and includes 0 ports.

Single Use Mixer (S.U.M.) BPC options include:
• Open top liner for media/buffer hydration
• Closed BPC with powder port for contained media/buffer hydration
• Closed BPC with powder port and monitoring probe capability
• Closed BPC for liquid-liquid mixing of critical sterile solutions such as pooling of fractions or processing of intermediate solutions
• Closed BPC for liquid-liquid mixing with monitoring probe capability

DHX™ Heat Exchanger BioProcess Container (BPC), ASI™ 26/77 film, ReadyMate connections, drain ports, one 6-L BPC (Thermo Scientific™)

The Thermo Scientific™ DHX™ Heat Exchanger Single-Use BioProcess Container (BPC) is a 2D system consisting of one 6 L BPC, with drain. It is composed of Thermo Scientific™ ASI™ 26/77 Polyethylene Film, with interconnecting C-Flex tubing and ReadyMate DAC end connections. The DHX Heat Exchanger and DHX BPC each features a serpentine pattern, creating a sterile fluid path for the process fluid. The BPC fits tightly between the stainless steel DHX Heat Exchanger plate system, and after loading, no further operator interaction is required. The customer has the option of utilizing up to 4 BPCs in the DHX unit.

Features
• Eliminates post-use cleaning steps required with reusable containers, helping to reduce cross-contamination risks
• Provides sterile processing environment without the cost and time of clean-in-place or steam-in-place sterilization (CIP/SIP)
• Minimizes bioburden by using the DHX Heat Exchanger unit and DHX BPCs to address weak points in a CIP system or sanitation process (typically required for stainless steel equipment)
• Reduces maintenance and life cycle cost due to its unique design
• Increases volume capacity via parallel connection between DHX units

Applications
• cGMP commercial and clinical biotherapeutics, vaccines, and other biologic processes
• Upstream applications, including media hold, harvest cooling, and harvest hold
• Downstream applications, including harvest hold, buffers, protein purification, and bulk drug substance

Three60™ Sampling System BioProcess Container (BPC) (Thermo Scientific™)

The simple design behind the Thermo Scientific™ Three60™ Sampling System allows you to take a representative sample of your product with minimal effort. The fluid path of the pre-irradiated BioProcess Container (BPC) has a quick-turn valve and pinch-and-cut disconnections for fluid transfer and removal. The 100 mL Three60 Sampling System BPC is composed of Thermo Scientific™ ASI™ 77 film, a 5-layer, 7 mL, polyethylene film and includes 2 ports (1 luer lock insert + cap, 1 injection site).

Features of the system include:
• Compatible with a 3.8 cm (1.5-inch) sanitary connector
• Requires no expensive hardware
• Each Three60 package contains a valve and four assemblies with pinch-and-cut disconnections
• Helps to eliminate post-use cleaning steps required with reusable containers, and to reduce cross-contamination risks

Customize standard BPC systems for optimized single-use technology
Choose from the industry’s largest component library, with over 2,000 unique components that include:
• Fittings—straight, reducing, elbow, T-style, X-style, and cross-style
• Connectors—quick connectors/disconnectors, aseptic connectors and disconnectors, and steam-to and steam-through connectors
• Filters (used for bioburden reduction)—sterilization grade filters and vent filters
• Tubing—thermoplastic elastomers and platinum-cured silicone
• Sample ports—septum-style and needle-free ports
• Clamps—sliders, pinch, and crimp bands

HyPerforma™ Smartainer™ Hardware, 1000L, Stainless steel outer container (Thermo Scientific™)

The 1000L Thermo Scientific™ HyPerforma™ Smartainer is a stainless-steel non-jacketed outer container system designed to accommodate large volume bioprocess containers.

Features:
• Durable containers are constructed from 304 stainless steel
• Ideal for in-house handling applications in critical areas
• Movable and stackable with pallet jack or forklift
• Non-jacketed
• Allow both top and bottom discharge from BPCs

HyPerforma™ Single-Use Fermentor Systems, 300L, Jacketed, AC motor, with 2-position center filter bracket (Thermo Scientific™)

The 300 L Thermo Scientific™ HyPerforma™ S.U.F., jacketed, AC Motor, with 2 position vent filter bracket and 120VAC electrical box, is designed to provide the users with enhanced functionality, ease of use, and efficiency for microbial fermentation. The HyPerforma S.U.F. system consists of a fermentor tank and Thermo Scientific™ HyPerforma™ S.U.F. BioProcess Container (BPC). The system has a 5:1 turndown ratio, which means it can run at a low 6 L working volume. The HyPerforma S.U.F. maintains traditional stirred-tank fermentation design principles, including specific height-to-diameter ratios (3:1), top-driven impeller location that deliver optimum cell viability, and performance and scalability from process development through production.

Features:
• The S.U.F. is a fully functional fermentor vessel including single-use sterile contact surfaces for mixing, venting, sparging and temperature sensing. Additional ports are included to allow choice of aseptic connections for filling, emptying and sampling.
• Unique proprietary mixing system enables conventional overhead mixing while maintaining sterility and integrity of the S.U.F. BPC.
• Minimized vessel footprint and reduced hold-up volumes.
• Simplified bag loading with a front access door.
• Tank includes baffles that enhance mixing.
• Side and bottom jacket for effective heat transfer.
• Cable management system offers greater organization for electrical cables, tubing and line sets.
• Dedicated adjustable tool holder keeps necessary tools available for convenience.
• BPC utilizes three Rushton-style impellers and sparger configuration with a proprietary exhaust management system.
• State-of-the-art foam sensor that helps to prevent exhaust filter failure.

Applications of the Thermo Scientific HyPerforma S.U.F. include:
• Proven for various microbial cultures of Pichia, Bacillus and others.
• Batch and fed-batch cultures.

Accessory:
Molded Probe Holder, Cat # SV50177P01

Thermo Scientific™ 5:1 Single-Use Bioreactor, jacketed, AC motor, 240VAC, e-box, analog load cells, 1000 L (Thermo Scientific™)

This 1000 L Thermo Scientific 5:1 Single-Use Bioreactor (SUB) is water-jacketed and includes AC motor, 240VAC, e-box, and analog load cells. It is designed using traditional stainless-steel bioreactor principles to ensure optimal cell culture performance. The SUB is a fully functional bioreactor vessel including single-use sterile contact surfaces for mixing, venting, sparging, and temperature sensing. The complete line of SUBs include 50, 100, 250, 500, 1000, and 2000-L sizes that ensure consistent scalability from pilot-scale studies, pre-clinical to commercial production.

Features:
• Unique proprietary mixing system that enables conventional overhead mixing while maintaining sterility and integrity of the SUB BioProcess Container (BPC)
• Largest qualified standard component library that allows for customization of SUB BPCs
• Minimized vessel footprint and reduced hold-up volumes
• Offered with AC or DC motors designed for precise agitation control
• Optional cable management tree provides line set and cabling organization for ease of use
• Load cells offered as standard feature with analog or digital communication options
• Option to use industry-leading single-use and traditional sensors for pH, DO, and pressure monitoring
• Option to choose the bioreactor vessel as a stand-alone system or complete turnkey system with dedicated controls from either the Finesse™ or Applikon™ families of control systems for bioproduction. These systems work on PC, Delta V™, Allen-Bradley™, or Siemens™ software platforms
• Updated 5:1 mixing turn-down ratio in combination with patented cross-flow sparger technology introduces gas just above liquid’s surface to facilitate efficient mass transfer CO2 stripping and reduce bubble sparging and associated foam damage to cells. This feature allows customers to do more with fewer vessels, which improves productivity and reduces costs simultaneously.

Applications of the Thermo Scientific SUB include:
• Batch, fed batch, and perfusion cultures
• Suspension and microcarrier cultures
• Proven for various cell lines such as CHO, Sp2/0 hybridoma, PER.C6, HEK293, Vero, MDCK, etc.

Accessories:
• Tube Assembly, Cat # SH3072002
• Molded Probe Holder, Cat # SV50177P01

HyPerforma™ Smartainer™ BPC Hoist, 1000L Smartainer System (Thermo Scientific™)

The Thermo Scientific™ HyPerforma™ BPC Hoist assists in handling and loading large volume bioprocess containers into Smartainer systems. Made of stainless steel this is intended for use with the 1000L Smartainer system.