Shop All Single-Use BioProcessing Systems

HyPerforma™ Glass Bioreactor, 120 V, heat and cool (Thermo Scientific™)

The Thermo Scientific HyPerforma Glass Bioreactor offers easy operation and rapid assembly and is manufactured with the highest standards for materials and surface finish. Developed using a computational fluid dynamics (CFD) simulator, the HyPerforma Glass Bioreactor impellers provide maximum mixing with minimum shear force, resulting in a higher average kLa. The cold finger has been replaced by a cooling loop to increase the thermal surface. Laboratory performance tests have demonstrated a cooling rate two times better than a sample of products from other suppliers. Control can be bidirectional, which allows a single-wall vessel to be used for mammalian applications and fermentation processes.

HyPerforma Glass Bioreactor features include:
• The motor adapter uses coupling windows and an alignment marker for easy assembly
• Ergonomic head plate design provides easy assembly and disassembly of components for rapid reconfiguration

Applications
• Suspension and adherent cell culture processes
• PD scale to full cGMP manufacturing workflows

HyPerforma™ 5:1 Single-Use Bioreactor, jacketed, AC motor, load cells, E-box, 240VAC (Thermo Scientific™)

This 100 L Thermo Scientific Single-Use Bioreactor (S.U.B.), water-jacketed, 240VAC, with AC motor, E-box, and analog load cells, is designed using traditional stainless-steel bioreactor principles to ensure optimal cell culture performance. The S.U.B. is a fully functional bioreactor vessel including single-use sterile contact surfaces for mixing, venting, sparging, and temperature sensing. The complete line of S.U.B.s include 50, 100, 250, 500, 1000, and 2000 L sizes that ensure consistent scalability from pilot-scale studies, pre-clinical to commercial production.

Features:
• Unique proprietary mixing system that enables conventional overhead mixing while maintaining sterility and integrity of the S.U.B. BioProcess Container (BPC)
• Largest qualified standard component library, which allows for customization of S.U.B. BPCs
• Minimized vessel footprint and reduced hold-up volumes
• Offered with AC or DC motors designed for precise agitation control
• Optional cable management tree provides line set and cabling organization for ease of use
• Load cells offered as standard feature with analog or digital communication options
• Option to use industry-leading single-use and traditional sensors for pH, DO, and pressure monitoring
• Option to choose the bioreactor vessel as a stand-alone system or a complete turnkey system with dedicated controls from either the Finesse™ or Applikon™ families of control systems for bioproduction. These systems work on PC, Delta V™, Allen-Bradley™, or Siemens™ software platforms.
• Updated 5:1 mixing turn-down ratio in combination with patented cross-flow sparger technology introduces gas just above liquid’s surface—to facilitate efficient mass transfer CO2 stripping, and reduce bubble sparging and associated foam damage to cells. This feature allows customers to do more with fewer vessels—which improves productivity and reduces costs simultaneously.

Applications of the Thermo Scientific S.U.B. include:
• Batch, fed batch, and perfusion cultures
• Suspension and microcarrier cultures
• Proven for various cell lines such as CHO, Sp2/0 hybridoma, PER.C6, HEK293, Vero, MDCK, etc.

HyPerforma™ Glass Bioreactor, 240 V, heat only (Thermo Scientific™)

The Thermo Scientific HyPerforma Glass Bioreactor offers easy operation and rapid assembly and is manufactured with the highest standards for materials and surface finish. Developed using a computational fluid dynamics (CFD) simulator, the HyPerforma Glass Bioreactor impellers provide maximum mixing with minimum shear force, resulting in a higher average kLa. The cold finger has been replaced by a cooling loop to increase the thermal surface. Laboratory performance tests have demonstrated a cooling rate two times better than a sample of products from other suppliers. Control can be bidirectional, which allows a single-wall vessel to be used for mammalian applications and fermentation processes.

HyPerforma Glass Bioreactor features include:
• The motor adapter uses coupling windows and an alignment marker for easy assembly
• Ergonomic head plate design provides easy assembly and disassembly of components for rapid reconfiguration

Applications
• Suspension and adherent cell culture processes
• PD scale to full cGMP manufacturing workflows

HyPerforma™ Mixtainer™ Hardware, Conical Drum, 50L (Thermo Scientific™)

The 50L Thermo Scientific™ Conical Drum, top dispense, with clamps is utilized as part of the Thermo Scientific™ HyPerforma™ Mixtainer™ system to support bioproduction mixing applications. Additional components of the system include the Thermo Scientific™ HyPerforma™ Mixtainer™ BioProcess Container (BPC) with an integrated magnetic stir bar, a magnetic stirring plate, a support plate and a dolly. The optimized support hardware allows mixing, moving, storage, shipping and discharge of the BPC contents while maintaining sterility of the contents. Standard products are available for sterile liquid-to-liquid or powder-to-liquid mixing.

Recommended for:
• Hydration of powdered media and buffers
• Sterile mixing of cell culture media with sera and reagents
• Sterile mixing of buffer solutions or other process fluids
• Pooling of sterile liquid fractions

Harvestainer™ BioProcess Container (BPC), 200L, with dual, inner 25L Microbarrier BPCs (Thermo Scientific™)

The 200L Thermo Scientific™ Harvestainer™ Microcarrier Separation BPC system is composed of Aegis5-14 single web, multi-layer plastic film and includes a dual inner bioprocess container (BPC), and 1 top port (MPX body), 1 bottom drain (MPX insert). This system enables the simple and effective separation of microcarriers from the cell culture supernatant harvest in a single-step, in a completely closed system. The Harvestainer BPC features a dual chamber system comprised of a 25L pillow-style BPC within the 2000L BPC. The inner Thermo Scientific™ Microcarrier Labtainer™ BPC, maximizes product recovery and microcarrier retention – the beads remain within this inner BPC while the supernatant collects in the outer 200L BPC.

Features:
• Use this system to eliminate post-use cleaning steps required with re-useable containers, and to reduce cross-contamination risks.
• All 3D BPCs are constructed in an ISO 7 cleanroom under cGMP conditions.
• Dual interior Microcarrier Labtainer option and Harvestainer BPC manifolding capability enable a spectrum of expansion – making the system scalable from process development to full cGMP commercial production.

Customize standard BPC systems for optimized single-use technology
Choose from the industry’s largest component library – with over 20,000 unique components that include:
Fittings—straight, reducing, elbow, T-style, X-style, and cross-style.
Connectors—quick connectors/disconnectors, aseptic connectors and disconnectors, steam-to and steam-through connectors.
Filters—used for bioburden reduction; sterilization grade filters and vent filters.
Tubing—thermoplastic elastomers, platinum-cured silicone.
Sample ports—septum-style and needle-free ports.
Clamps—sliders, pinch, and crimp bands.

HyPerforma™ Glass Bioreactor, 240 V, heat only, with scale (Thermo Scientific™)

The Thermo Scientific HyPerforma Glass Bioreactor offers easy operation and rapid assembly and is manufactured with the highest standards for materials and surface finish. Developed using a computational fluid dynamics (CFD) simulator, the HyPerforma Glass Bioreactor impellers provide maximum mixing with minimum shear force, resulting in a higher average kLa. The cold finger has been replaced by a cooling loop to increase the thermal surface. Laboratory performance tests have demonstrated a cooling rate two times better than a sample of products from other suppliers. Control can be bidirectional, which allows a single-wall vessel to be used for mammalian applications and fermentation processes.

HyPerforma Glass Bioreactor features include:
• The motor adapter uses coupling windows and an alignment marker for easy assembly
• Ergonomic head plate design provides easy assembly and disassembly of components for rapid reconfiguration

Applications
• Suspension and adherent cell culture processes
• PD scale to full cGMP manufacturing workflows

BioProcess Container (BPC), 1000L, for HyPerforma™ Single-Use Mixer, liquid-to-liquid, Aegis5-14 film, 3 ports (Thermo Scientific™)

The 1000L Thermo Scientific™ Bioprocess Containers (BPC) for liquid-to-liquid mixing, is composed of Aegis 5-14 single web, multi-layer plastic film and includes 3 ports.

Single Use Mixer (S.U.M.) BPC options include:
• Open top liner for media/buffer hydration
• Closed BPC with powder port for contained media/buffer hydration
• Closed BPC with powder port and monitoring probe capability
• Closed BPC for liquid-liquid mixing of critical sterile solutions such as pooling of fractions or processing of intermediate solutions
• Closed BPC for liquid-liquid mixing with monitoring probe capability

HyPerforma™ 5:1 Single-Use Bioreactor, 304, jacketed, AC motor, load cells (Thermo Scientific™)

The Thermo Scientific HyPerforma 5:1 Single-Use Bioreactor (S.U.B.), 304, water-jacketed, AC motor, with load cells, is designed using traditional stainless steel bioreactor principles to ensure optimal cell culture performance. The S.U.B. is a fully functional bioreactor vessel that includes single-use sterile contact surfaces for mixing, venting, sparging, and temperature sensing. The complete line of S.U.B.s includes 50, 100, 250, 500, 1,000 and 2,000 L sizes that ensure consistent scalability from pilot-scale studies to pre-clinical studies to commercial production.

HyPerforma Single-Use Bioreactor features include:
• Unique proprietary mixing system that enables conventional overhead mixing while maintaining sterility and integrity of the S.U.B. bioprocess container (BPC)
• Largest qualified standard component library that allows for customization of S.U.B. BPCs
• Minimized vessel footprint and reduced hold-up volumes
• Load cells offered as standard feature with analog or digital communication options
• Option to use industry-leading single-use and traditional sensors for pH, DO, and pressure monitoring
• Updated 5:1 mixing turn-down ratio in combination with patented cross-flow sparger technology introduces gas just above liquid surface to facilitate efficient mass transfer CO2 stripping and reduce bubble sparging and associated foam damage to cells. This enables the use of fewer vessels, which improves productivity and reduces costs simultaneously.

Applications
• Suspension and adherent cell culture processes
• Fermentation processes
• PD scale to full cGMP manufacturing workflows

Accessories for HyPerforma™ Single-Use Bioreactors, Bioreactor Probe Assembly (Thermo Scientific™)

The Thermo Scientific™ Bioreactor Probe Assembly (nonsterile) is used to package the pH and DO probes for sterilization and to aseptically connect them to the BPCs for Thermo Scientific™ HyPerforma™ Single-Use Bioreactors.

HyPerforma™ Glass Bioreactor, 240 V, heat and cool, with scale (Thermo Scientific™)

The Thermo Scientific HyPerforma Glass Bioreactor offers easy operation and rapid assembly and is manufactured with the highest standards for materials and surface finish. Developed using a computational fluid dynamics (CFD) simulator, the HyPerforma Glass Bioreactor impellers provide maximum mixing with minimum shear force, resulting in a higher average kLa. The cold finger has been replaced by a cooling loop to increase the thermal surface. Laboratory performance tests have demonstrated a cooling rate two times better than a sample of products from other suppliers. Control can be bidirectional, which allows a single-wall vessel to be used for mammalian applications and fermentation processes.

HyPerforma Glass Bioreactor features include:
• The motor adapter uses coupling windows and an alignment marker for easy assembly
• Ergonomic head plate design provides easy assembly and disassembly of components for rapid reconfiguration

Applications
• Suspension and adherent cell culture processes
• PD scale to full cGMP manufacturing workflows

Three60™ Sampling System BioProcess Container (BPC) (Thermo Scientific™)

The simple design behind the Thermo Scientific™ Three60™ Sampling System allows you to take a representative sample of your product with minimal effort. The fluid path of the pre-irradiated BioProcess Container (BPC) has a quick-turn valve and pinch-and-cut disconnections for fluid transfer and removal. The 1 L Three60 Sampling System BPC is composed of Thermo Scientific™ ASI™ 77 film, a 5-layer, 7 mL, polyethylene film and includes 2 ports (1 luer lock insert + cap, 1 injection site).

Features of the system include:
• Compatible with a 3.8 cm (1.5-inch) sanitary connector
• Requires no expensive hardware
• Each Three60 package contains a valve and four assemblies with pinch-and-cut disconnections
• Helps to eliminate post-use cleaning steps required with reusable containers, and to reduce cross-contamination risks

Customize standard BPC systems for optimized single-use technology
Choose from the industry’s largest component library, with over 2,000 unique components that include:
• Fittings—straight, reducing, elbow, T-style, X-style, and cross-style
• Connectors—quick connectors/disconnectors, aseptic connectors and disconnectors, and steam-to and steam-through connectors
• Filters (used for bioburden reduction)—sterilization grade filters and vent filters
• Tubing—thermoplastic elastomers and platinum-cured silicone
• Sample ports—septum-style and needle-free ports
• Clamps—sliders, pinch, and crimp bands

BioProcess Container (BPC), 50L, for HyPerforma™ Single-Use Mixer DS 300, Bottom drain, irradiated (Thermo Scientific™)

The 50L closed-top, irradiated BioProcess Container (BPC) with bottom drain is composed of CX3-9 single web, multi-layer plastic film and that fit inside drums for Thermo Scientific™ HyPerforma Single-Use Mixer DS 300. Implement industry-proven BPCs and liners in the overall process of shipping and storing your liquid-liquid and powder-liquid bioprocess mixing applications. BPCs and liners come in open- and closed-top design and are manufactured using our proprietary CX5-14 and CX3-9 film that easily fit in every plastic drum size.

Key features of HyPerforma Single-Use Mixer DS 300 BPCs and liners:
• All standard BPCs and liners are designed to fit “out-of-the-box“
• Available in volume options of 50, 100, and 200L
• Applicable to open- and closed-top mixing applications
• Powder-liquid and liquid-liquid mixing
• World class CX5-14 and CX3-9 film
• Adaptable to multiple upstream process applications in media and buffer prep

Required accessories:
• HyPerforma Single-Use Mixer DS 300 docking station
• HyPerforma SIngle-Use Mixer DS 300 drums

Recommended for:
• Powder-liquid or liquid-liquid mixing applications in single use
• High-throughput mixing and storage
• Additional configurations are available to suit your application requirements.

Accessory: Reusable Bearing Hub, Cat # SV5017777

HyPerforma™ Single-Use Mixer, 50 L, jacketed, DC motor, touchscreen console, with load cells (Thermo Scientific™)

The Thermo Scientific HyPerforma Single-Use Mixer (S.U.M.) with touchscreen console is designed using proven mixer platform principles and used for upstream and downstream mixing applications. The HyPerforma S.U.M. offers an ergonomic and efficient user experience. The complete line of HyPerforma single-use mixers includes 50, 100, 200, 500, 1000 and 2000 L sizes, helping ensure consistent scalability for a number of applications from powder media and buffer hydration to advanced downstream harvest and intermediate pooling steps. The touchscreen console provides an unsurpassed user experience while providing agitation, pump, and TCU control, as well as easy-to-monitor pH, conductivity, temperature, mass, and pressure measurements. The touchscreen console also provides the capability for automated acid, base, and saline titration and automated vessel fill and harvest.

HyPerforma S.U.M. features include:
• Unique proprietary mixing system that provides conventional overhead mixing while maintaining sterility and integrity of the S.U.M. BioProcess Container (BPC)
• Modular graphical touchscreen user interface allows easy viewing of mass, pH, conductivity, temperature, and pressure measurements, and agitation, pump control, and temperature settings (if applicable, for jacketed vessel)
• Data management through Modbus, Profibus, Ethernet, and USB
• Automated acid, base, and saline titration
• Automated mixer fill and harvest control
• Recipe development and storage capabilities
• Simple bag loading with a vertical access door (available on 500 L, 1000 L, and 2000 L sizes)
• Minimized vessel footprint and reduced hold-up volume
• Cable management system provides greater organization for electrical cables, tubing, and line sets
• Dedicated adjustable tool holder keeps necessary tools available
• Mixer vessel is offered non-jacketed (no heat transfer) or with a water jacket (vessel side and bottom). Water jacket is used with separate external temperature control unit for heat transfer requirement.
• Three load cell weighing systems enable accurate batch weight monitoring
• Option to use probes for temperature, pH, conductivity, and pressure monitoring
• S.U.M. BPCs offered in CX5-14 and Aegis5-14 films

Applications of the HyPerforma S.U.M. include:
• Powder media hydration
• Liquid media concentrate dilution
• Harvest hold tank
• Viral inactivation
• Downstream intermediate pooling
• Final product hold and additive suspension

imPULSE™ Single-Use Mixer (S.U.M.) (Thermo Scientific™)

The 30 L Thermo Scientific™ imPULSE™ Single-Use Mixer (S.U.M.) was designed with proven engineered mixer principles and can be used for upstream and downstream mixing applications. The linear-scale design delivers uniform, superior mixing in every model, from 30 L to 5000 L, and mixes to empty. The mixer provides consistent scalability and can be used in numerous applications—from powder media and buffer hydration to advanced downstream harvest and intermediate pooling steps.

The imPULSE Core
The innovative disc design and the hexagonal shape of the disc and vessel are at the core of the quick and thorough mixing capabilities of the imPULSE series. The imPULSE Core line offers superior mixing without high-end controls and monitors. Some basic features and options include:
• Linear mixing scalability from 30 L to 5,000 L
• Hexagonal vessel with open top and sloped bottom with openings for the mixing BPC and tubing outlets
• Integrated control panel with relay logic, manual push buttons, selector switch interface, and digital speed indication
• Stationary powder BPC hanger and lifting system with manual or power winch
• Mixing tank jacket and insulation
• Weight indication system with load cells
• Auto-inflate and vent control options

Applications
• Media preparation
• Final formulation steps
• Buffer preparation
• Use as a harvest vessel
• Pooling and liquid transfer
• Product suspension
• Mixing and storage of multiple batches
• Viral inactivation

Accessories:
• Tube Assembly, Cat # SH3072002
• Molded Probe Holder, Cat # SV50177P01

Plastic Drum, 50L, for HyPerforma™ Single-Use Mixer DS 300, with Conical Insert (Thermo Scientific™)

The 50L Plastic Drum with Conical Insert adds speed and convenience to your workflow. The drum is designed for use with the Thermo Scientific™ HyPerforma™ Single-Use Mixer (S.U.M.) DS 300 and is easily moved to cold rooms for storage.

Key features:
• Standard plastic drums available in 50, 100, 200, and 300L sizes
• Lightweight for ease of mobility
• Applicable to open- and closed-top mixing applications
• Economical
• Bottom drain for easy transfer
• 5:1 turn down ratio in conjunction with docking station and a bioprocess container bag or liner

Optional:
• Dollies for the drums are available in two sizes: one for 50 to 200L drums and another for 300L drums. These drums should be used with the corresponding optional drum positioner that is available with the docking station.

Required accessories:
• HyPerforma Single-Use Mixer DS 300 docking station
• HyPerforma Single-Use Mixer DS 300 bioprocess container bags for closed-top mixing or liners for open-top mixing

Recommended for:
• Powder-liquid or liquid-liquid mixing applications in single-use
• High-throughput mixing and storage