Qubit 4 Fluorometer

Fast and sensitive quantification of DNA, RNA, and Protein

The Invitrogen Qubit 4 Fluorometer is the next generation of the popular benchtop fluorometer designed to accurately measure DNA, RNA, and protein quantity. The new Qubit 4 also easily measures RNA integrity and quality. The easy-to-use touch screen menus make it easy to select and run the assays you need, with results displayed in just a few seconds.

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Key features of the Qubit 4 Fluorometer include:

  • More sensitive than UV absorbance–based quantification, making it ideal for precious samples
  • Accurately quantifies DNA, RNA, and protein in <3 seconds per sample
  • Fast, reliable detection of degraded RNA with the new RNA IQ assay
  • New integrated reagent calculator reports amount of dye and buffer needed
  • Uses as little as 1 μL of sample
  • Flexible options for exporting results: Wi-Fi, USB drive, or direct connection with a USB cable

The latest version of the Qubit fluorometer has been re-engineered to run the Qubit RNA IQ (integrity & quality) assay. The concentration or quality of the target molecule in the sample is reported by a fluorescent dye that emits a signal only when bound to the target, which minimizes the effects of contaminants on the result.

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How the Qubit Fluorometer works

Single-sample measurements are easy

This user-friendly fluorometer delivers results using quick, simple assay procedures. The large, color touch screen allows you to easily navigate through the various options. Calculations and settings are automatically performed by the instrument (Figure 1). The Qubit 4 Fluorometer will save the data for up to 1,000 samples, and you can transfer data using Wi-Fi, a USB drive, or direct connection with a USB cable. In addition, new Qubit 4 includes a built-in reagent calculator to easily determine the amount of dye and buffer required to prepare your samples and standards (Figure 2).

Qubit 4 Fluorometer workflow.

Figure 1. Qubit 4 Fluorometer workflow.

Integrated reagent calculator

Figure 2. Integrated reagent calculator. Enter the number of samples and standards needed to calculate the amount of dye and buffer.

Assays for use with the Qubit Fluorometer

The Qubit assays for use with the Qubit Fluorometer are all performed using the same general protocol, which utilizes a simple mix-and-read format, with incubation times of only 2 minutes required for DNA and RNA assays or 15 minutes for the protein assay (Figure 3). In addition to our core assays for dsDNA, oligos, total RNA, microRNA, and protein, Ion Sphere Particle quality can be evaluated on the Qubit 4 Fluorometer using the Ion Sphere Quality Control Kit prior to performing a sequencing run on the Ion Personal Genome Machine (PGM) Sequencer.

See all Qubit assays

Effective quantifiable range for each Qubit nucleic acid assay

Figure 3. Comparison of sample concentration ranges for the Qubit assays and UV absorbance measurements. UV absorbance readings are not selective for RNA vs DNA.

 

Sensitive, accurate biomolecule quantification

The Qubit Fluorometer utilizes fluorescent dyes that are specific to the target of interest. These fluorescent dyes emit only when bound to the target molecules, even at low concentrations (Figure 4). The Qubit 4 Fluorometer is more sensitive than UV absorbance, which measures anything absorbing at 260 nm—DNA, RNA, protein, free nucleotides, or excess salts. Moreover, UV spectrophotometry often does not have the sensitivity to accurately measure low concentrations of DNA and RNA (Figure 5). With the Qubit Fluorometer, your research is enhanced by more accurate measurements because the dyes in the Invitrogen Qubit assay kits fluoresce only when bound to the selected molecule—DNA, RNA, or protein—in your sample. This allows you to avoid repeating work due to inaccurate measurements.

Performance of the Qubit dsDNA HS Assay

Figure 4. Performance of the Qubit dsDNA HS Assay. The Invitrogen Qubit dsDNA HS Assay has a linear detection range of 0.2–100 ng and is selective for dsDNA, even in the presence of an equal mass of RNA.

Accuracy and precision of the Qubit Quantitation Platform

Figure 5. Accuracy and precision of the Qubit Quantification Platform. Ten replicates of lambda DNA at concentrations from 0.01 to 10 ng/μL were assayed using the Qubit dsDNA HS Assay on the Qubit Fluorometer according to the standard kit protocol. The same concentrations of DNA were measured in 10 replicates using a microvolume spectrophotometer, and results were compared for both accuracy and precision. Each bar represents the average of 10 replicates. Error bars represent the standard deviations of the 10 replicates. The concentrations indicated are the concentrations of DNA in the starting samples, before dilution in the Qubit assay tubes.

Qubit Fluorometer product guide

Qubit 4 Fluorometer Qubit 4 Quantification Starter Kit Qubit 4 NGS Starter Kit Qubit 4 RNA IQ Starter Kit
Qubit 4 Fluorometer Qubit 4 Quantitation Starter Kit Qubit 4 NGS Starter Kit Qubit 4 RNA IQ Starter Kit
  • Qubit 4 Fluorometer
  • 1 international power adaptor
  • Microfiber cloth
  • USB drive and USB cable
  • Qubit 4 Fluorometer
  • Qubit 4 System Verification Assay Kit (50 assays)
  • 1 dsDNA HS (high sensitivity) Assay Kit (100 assays)
  • 1 dsDNA BR (broad range) Assay Kit (100 assays)
  • 1 RNA IQ (integrity and quality) Assay Kit (75 assays)
  • 1 RNA HS Assay Kit (100 assays)
  • 1 RNA (extended range) Assay Kit (100 assays)
  • 1 Protein Assay Kit (100 assays)
  • Qubit Assay Tubes (500)
  • 1 international power adaptor
  • Microfiber cloth
  • USB drive and USB cable
  • Qubit 4 Fluorometer
  • 1 dsDNA HS (high sensitivity) Assay Kit (500 assays)
  • Qubit Assay Tubes (500)
  • 1 international power adaptor
  • Microfiber cloth
  • USB drive and USB cable
  • Qubit 4 Fluorometer
  • 1 RNA IQ (integrity & quality) Assay Kit (275 assays)
  • 1 international power adaptor
  • Microfiber cloth
  • USB drive and USB cable
Q33241
Featured video: Nucleic Acid Quantification—Comparing Qubit fluorometer to the Nanodrop One UV/Vis absorbance

The best of both worlds

The combination of the Thermo Scientific NanoDrop One UV-Vis Spectrophotometer and the Qubit Fluorometer enables accurate, easy, and fast quantification of biomolecules.

Learn more about Nucleic acid quantitation

System specifications

Instrument dimensions 5.4 in (w) x 10 in (l) x 2.2 in (h)
(13.6 cm x 25 cm x 5.5 cm)
Weight 743 g
Dynamic range 5 orders of magnitude
Processing time ≤5 seconds/sample
Light sources Blue LED (max ~470 nm)
Red LED (max ~635 nm)
Excitation filters Blue 430–495 nm
Red 600–645 nm
Emission filters Green 510–580 nm
Red 665–720 nm
Detectors Photodiodes: measurement capability from 300–1,000 nm
Warm-up time <35 seconds
USB drive 4 GB

Fluorometer mode

By switching to Fluorometer mode, the Qubit Fluorometer can be used as a mini-fluorometer. In Fluorometer mode, rather than performing an assay calibration and calculating results based on an algorithm, the instrument generates and displays raw fluorescence (RFU) values for each sample.

Fluorometer mode allows you to manually select the excitation source: blue LED or red LED. If blue excitation is chosen, the instrument reads the fluorescence in both the green and far red emission channels; if red excitation is chosen, emission is read in the far-red channel only.

Data gathered in Fluorometer mode may be used to design your own assay. See MyQubit for more information.

Step 1. Access Fluorometer mode

Step 1. Access Fluorometer mode. To access Fluorometer mode, press the Fluorometer button from the home screen.

Step 2. Choose excitation

Step 2. Choose excitation Choose Blue (470 nm) or Red (635 nm) excitation from the menu.

Step 3. Insert sample.

Step 3. Insert sample. Insert your sample and press Read tube.

Step 4. Read tube For Blue excitation, the Qubit Fluorometer displays both the green emission and the far-red emission of your sample.

Step 4. Read tube For Blue excitation, the Qubit Fluorometer displays both the green emission and the far-red emission of your sample. For Red excitation, you will see the far-red emission of your sample. Remove the tube and press Read tube to read the next sample.

MyQubit—Envision and create new assays for the Qubit 4 Fluorometer

The Qubit 4 Fluorometer is compatible with the MyQubit assay design tool. MyQubit allows you to create new assays for the Qubit 4 Fluorometer in minutes. Simply enter your assay parameters into the online tool, and then save and upload the .qbt file to the Qubit 4 Fluorometer.

Learn more about MyQubit

Invitrogen Qubit 4 Fluorometer: Quantitation

Invitrogen Qubit 4 Fluoromoeter: Managing Data

Invitrogen Qubit 4 Fluorometer: Additional Features

Invitrogen Qubit 4 and RNA Quantitation and Qualification

Quantitation: Comparing Qubit fluorometer to the Nanodrop One UV/Vis absorbance

Qubit Fluorometer product guide

Qubit 4 Fluorometer Qubit 4 Quantification Starter Kit Qubit 4 NGS Starter Kit Qubit 4 RNA IQ Starter Kit
Qubit 4 Fluorometer Qubit 4 Quantitation Starter Kit Qubit 4 NGS Starter Kit Qubit 4 RNA IQ Starter Kit
  • Qubit 4 Fluorometer
  • 1 international power adaptor
  • Microfiber cloth
  • USB drive and USB cable
  • Qubit 4 Fluorometer
  • Qubit 4 System Verification Assay Kit (50 assays)
  • 1 dsDNA HS (high sensitivity) Assay Kit (100 assays)
  • 1 dsDNA BR (broad range) Assay Kit (100 assays)
  • 1 RNA IQ (integrity and quality) Assay Kit (75 assays)
  • 1 RNA HS Assay Kit (100 assays)
  • 1 RNA (extended range) Assay Kit (100 assays)
  • 1 Protein Assay Kit (100 assays)
  • Qubit Assay Tubes (500)
  • 1 international power adaptor
  • Microfiber cloth
  • USB drive and USB cable
  • Qubit 4 Fluorometer
  • 1 dsDNA HS (high sensitivity) Assay Kit (500 assays)
  • Qubit Assay Tubes (500)
  • 1 international power adaptor
  • Microfiber cloth
  • USB drive and USB cable
  • Qubit 4 Fluorometer
  • 1 RNA IQ (integrity & quality) Assay Kit (275 assays)
  • 1 international power adaptor
  • Microfiber cloth
  • USB drive and USB cable
Q33241
Featured video: Nucleic Acid Quantification—Comparing Qubit fluorometer to the Nanodrop One UV/Vis absorbance

The best of both worlds

The combination of the Thermo Scientific NanoDrop One UV-Vis Spectrophotometer and the Qubit Fluorometer enables accurate, easy, and fast quantification of biomolecules.

Learn more about Nucleic acid quantitation

System specifications

Instrument dimensions 5.4 in (w) x 10 in (l) x 2.2 in (h)
(13.6 cm x 25 cm x 5.5 cm)
Weight 743 g
Dynamic range 5 orders of magnitude
Processing time ≤5 seconds/sample
Light sources Blue LED (max ~470 nm)
Red LED (max ~635 nm)
Excitation filters Blue 430–495 nm
Red 600–645 nm
Emission filters Green 510–580 nm
Red 665–720 nm
Detectors Photodiodes: measurement capability from 300–1,000 nm
Warm-up time <35 seconds
USB drive 4 GB

Fluorometer mode

By switching to Fluorometer mode, the Qubit Fluorometer can be used as a mini-fluorometer. In Fluorometer mode, rather than performing an assay calibration and calculating results based on an algorithm, the instrument generates and displays raw fluorescence (RFU) values for each sample.

Fluorometer mode allows you to manually select the excitation source: blue LED or red LED. If blue excitation is chosen, the instrument reads the fluorescence in both the green and far red emission channels; if red excitation is chosen, emission is read in the far-red channel only.

Data gathered in Fluorometer mode may be used to design your own assay. See MyQubit for more information.

Step 1. Access Fluorometer mode

Step 1. Access Fluorometer mode. To access Fluorometer mode, press the Fluorometer button from the home screen.

Step 2. Choose excitation

Step 2. Choose excitation Choose Blue (470 nm) or Red (635 nm) excitation from the menu.

Step 3. Insert sample.

Step 3. Insert sample. Insert your sample and press Read tube.

Step 4. Read tube For Blue excitation, the Qubit Fluorometer displays both the green emission and the far-red emission of your sample.

Step 4. Read tube For Blue excitation, the Qubit Fluorometer displays both the green emission and the far-red emission of your sample. For Red excitation, you will see the far-red emission of your sample. Remove the tube and press Read tube to read the next sample.

MyQubit—Envision and create new assays for the Qubit 4 Fluorometer

The Qubit 4 Fluorometer is compatible with the MyQubit assay design tool. MyQubit allows you to create new assays for the Qubit 4 Fluorometer in minutes. Simply enter your assay parameters into the online tool, and then save and upload the .qbt file to the Qubit 4 Fluorometer.

Learn more about MyQubit

Invitrogen Qubit 4 Fluorometer: Quantitation

Invitrogen Qubit 4 Fluoromoeter: Managing Data

Invitrogen Qubit 4 Fluorometer: Additional Features

Invitrogen Qubit 4 and RNA Quantitation and Qualification

Quantitation: Comparing Qubit fluorometer to the Nanodrop One UV/Vis absorbance

Product Initial sample concentration Quantitation range Quantity Cat. No.
DNA quantitation kits
Qubit ssDNA Assay Kit 50 pg/μL to 200 ng/μL 1–200 ng 100 assays Q10212
Qubit dsDNA BR Assay Kit 100 pg/μL to 1,000 ng/μL 2–1,000 ng 100 assays Q32850
500 assays Q32853
Qubit dsDNA HS Assay Kit 10 pg/μL to 100 ng/μL 0.2–100 ng 100 assays Q32851
500 assays Q32854
Qubit 1X dsDNA HS Assay Kit 10 pg/μL to 100 ng/μL 0.2–100 ng 100 assays Q33230
500 assays Q33231
RNA integrity and quality kit
Qubit RNA IQ Assay Kit* NA NA 75 assays Q33221
275 assays Q33222
RNA quantitation kits
Qubit RNA BR Assay Kit 1 ng/μL to 1 μg/μL 20–1,000 ng 100 assays Q10210
500 assays Q10211
Qubit RNA HS Assay Kit 250 pg/μL to 100 ng/μL 5–100 ng 100 assays Q32852
500 assays Q32855
Qubit RNA XR Assay Kit 1 ng/μL to 8 μg/μL 20 ng–8 μg 100 assays Q33223
500 assays Q33224
Qubit microRNA Assay Kit 50 ng/mL to 100 μg/mL 1–1,000 ng 100 assays Q32880
500 assays Q32881
Protein quantitation kit
Qubit Protein Assay Kit 12.5 μg/mL to 5 mg/mL 0.25–5 μg 100 assays Q33211
500 assays Q33212
Instrument and accessories
Qubit 4 Fluorometer 1 instrument Q33226
Qubit 4 System Verification Assay Kit 1 kit Q33237
Qubit 4 RNA IQ Starter Kit 1 kit Q33229
Qubit 4 Quantitation Starter Kit 1 kit Q33227
Qubit 4 NGS Starter Kit 1 kit Q33228

* Note: The Qubit RNA IQ Assay for the detection of degraded RNA can only be run on the Qubit 4 Fluorometer and cannot be performed on the original Qubit, Qubit 2.0, or Qubit 3.0 Fluorometers.

Resources

Product documentation

Technical and application notes

Info sheets

Featured articles

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Additional resources