EVOS M5000 Imaging System

AMF5000SV is available in North America only.

The EVOS M5000 microscope is a workhorse for cell imaging labs across a broad range of applications and imaging requirements. With its powerful software tools, the EVOS M5000 helps revolutionize your imaging workflow.

The Stage View feature allows you to track your location as you manually move the stage and search for your cells. Areas of interest can be marked, saved, and returned to, at any time. Designed for biologists by biologists, the EVOS M5000 with Stage View is remarkably easy to use.

Features

In addition to the common features of all EVOS microscopes, the EVOS M5000 offers:

Easy self-installation

No maintenance, assembly, alignment, or calibration

Advanced color options

User-defined 4-color fluorescence, transmitted light, and color images

Thermo Fisher Connect™ connectivity

Images can be uploaded directly from the EVOS M5000 instrument to a Thermo Fisher Connect account. Any new software upgrade can also be downloaded directly in connected instruments. Additional post-acquisition image analysis can be performed via the EVOS Image Analysis application on Thermo Fisher Connect.

Location tracking intelligence

EVOS Stage View software functionality for tracking of stage location.

Optimal Performance

Autofocus, Z-stack capability, time-lapse imaging, and single-click multichannel capture

Biosafety cabinet compatible

Can be used within a biosafety cabinet or hood

EVOS Onstage Incubator

Compatibility for live-cell analysis with precise control of temperature, humidity, and gases

Automation

Automated cell counting and confluence measurements in the onboard software

Enhanced safety

Most operational functions can be controlled through software, eliminating the need to touch the instrument except to mount the slide and adjust the stage

Celleste Image Analysis Software option

Compatibility for advanced image processing and analysis, including 2D and 3D deconvolution and 3D visualization and analysis

Demo videos & customer stories

View more EVOS systems videos and demos under Resources.

Featured Images

11-slice image Z-stack of honeybee claw

EVOS M5000 in color mode, 10x Apo objective.

Differentiated mouse embryonic stem cells

EVOS M5000. 10x objective.

HeLa cells with stained mitochondria

EVOS M5000. 40x Fluorite objective.

Human adenocarcinoma section

EVOS M5000 in color mode, 20X objective.

Section of rat intestine

Section of rat intestine EVOS M5000, RGB mode illumination. 10x objective.

View more M5000 images and movies in our Image Gallery and Video Gallery.

Specifications

For Research Use Only. Not for use in diagnostic procedures.

Category	Attribute	AMF5000	AMF5000SV*
Optics	Description	Infinity-corrected optical system; RMS threaded objectives with 45 mm parfocal distance	Infinity-corrected optical system; RMS threaded objectives with 45 mm parfocal distance
	Imaging mode	Fluorescence, brightfield, color brightfield, phase contrast	Fluorescence, brightfield, color brightfield, phase contrast
	Imaging methods	Single color, multicolor, time lapse, Z-stacking, movie capture	Single color, multicolor, time lapse, Z-stacking, movie capture
	Illumination	Adjustable-intensity LED light cubes with integrated hard-coated filter set and >50,000-hour life	Adjustable-intensity LED light cubes with integrated hard-coated filter set and >50,000-hour life
	Light cube capacity	4-position chamber, for 4 fluorescence cubes + brightfield	4-position chamber, for 4 fluorescence cubes + brightfield
	Light cubes (not included)	Broad selection of standard and specialty light cubes. Commonly used light cubes include (ex/em): • DAPI (357/447 nm) • GFP (470/525 nm) • RFP (531/593 nm) • Texas Red (585/624 nm) • Cy5 (628/692 nm)	Broad selection of standard and specialty light cubes. Commonly used light cubes include (ex/em): • DAPI (357/447 nm) • GFP (470/525 nm) • RFP (531/593 nm) • Texas Red (585/624 nm) • Cy5 (628/692 nm)
	Objective capacity	5-position manual turret, not automated	5-position manual turret, not automated
	Objectives (not included)	Wide selection of high-quality long working distance (LWD) and coverslip-corrected objectives	Wide selection of high-quality long working distance (LWD) and coverslip-corrected objectives
	Condenser	60-mm LWD condenser; 4-position turret with a clear aperture and 3 phase annuli	60-mm LWD condenser; 4-position turret with a clear aperture and 3 phase annuli
	Focus mechanism	Automated focus with sub-micron (0.150 µm) resolution (single-step accuracy)	Automated focus with sub-micron (0.150 µm) resolution (single-step accuracy)
	Monochrome camera	High-sensitivity 3.2 MP (2048 x 1536) CMOS sensor with 3.45 µm pixel resolution	High-sensitivity 3.2 MP (2048 x 1536) CMOS sensor with 3.45 µm pixel resolution
	Captured images	16-bit RAW monochrome: TIFF, PNG (12-bit dynamic range) 8-bit color: TIFF, PNG, JPG Movies and time-lapse: AVI, WMV	16-bit RAW monochrome: TIFF, PNG (12-bit dynamic range) 8-bit color: TIFF, PNG, JPG Movies and time-lapse: AVI, WMV
Physical characteristics	Power supply	24V AC adapter with country-specific power cords**	24V AC adapter with country-specific power cords**
	Dimensions (W x H x D)	18 x 23 x 18 inches 45.7 x 58.4 x 45.7 cm	18 x 23 x 18 inches 45.7 x 58.4 x 45.7 cm
	Weight	36 lb (16.4 kg)	36 lb (16.4 kg)
Stage	X/Y stage	Travel range 120 mm x 80 mm with sub-micron resolution Drop-in inserts to receive vessel holders	Travel range 120 mm x 80 mm with sub-micron resolution Drop-in inserts to receive vessel holders
	Stage mechanism	Mechanical	Mechanical
	Stage tracking	N/A	Proprietary functionality to track stage locations in the EVOS Stage View software
System	Computer	Embedded PC with 4 GB RAM	Embedded PC with 4 GB RAM
	Storage	10 GB SSD 16 GB USB 3.0 memory stick	10 GB SSD 16 GB USB 3.0 memory stick
	Output ports	Power; 4 x USB 2.0; 1 x USB 3.0; 1 Display Port; 1 RJ45	Power; 4 x USB 2.0; 1 x USB 3.0; 1 Display Port; 1 RJ45
	Networking capability	Connection through Windows/SMB network via Ethernet cable connection USB 3.0 WiFi dongle	Connection through Windows/SMB network via Ethernet cable connection USB 3.0 WiFi dongle
AMF5000SV is now available for pilot sales in selected regions. *In regions not using the Type B (North America) power plug, one (1) country-specific power cord must be ordered separately: AMEP4646 EVOS Power Cord, Type C (Europe) AMEP4708 EVOS Power Cord, Type D (India/South Africa) AMEP4645 EVOS Power Cord, Type G (United Kingdom) AMEP4649 EVOS Power Cord, Type H (Israel) AMEP4647 EVOS Power Cord, Type I (Australia) AMEP4782 Power Cord, Type I (China, CCC certified) AMEP4786 Power Cord Type J (Switzerland) AMEP4648 EVOS Power Cord, Type L (Italy)