EVOS M5000 Imaging System

Catalog number: AMF5000

The EVOS M5000 microscope is a workhorse for cell imaging labs across a broad range of applications and imaging requirements. Semi-automation, powerful software tools, and a user interface designed by biologists for biologists, means that the M5000 can revolutionize your imaging workflow.


With a high-resolution CMOS monochrome camera capable of four-color fluorescence, transmitted light, and color applications, the EVOS M5000 delivers publication-ready images in seconds with minimal personal contact.

Explore EVOS M5000 360º view

Check virtually EVOS M5000 in 360º view before actual demo.


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In addition to the common features of all EVOS microscopes, the EVOS M5000 offers:

Easy self-installation

No maintenance, assembly, alignment, or calibration

Advanced color options

User-defined 4-color fluorescence, transmitted light, and color images

Thermo Fisher Connect™ connectivity

Images can be uploaded directly from the EVOS M5000 instrument to a Thermo Fisher Connect account. Any new software upgrade can also be downloaded directly in connected instruments. Additional post-acquisition image analysis can be performed via the EVOS Image Analysis application on Thermo Fisher Connect.

Optimal Performance

Autofocus, Z-stack capability, time-lapse imaging, and single-click multichannel capture

Biosafety cabinet compatible

Can be used within a biosafety cabinet or hood


EVOS Onstage Incubator

Compatibility for live-cell analysis with precise control of temperature, humidity, and gases


Automated cell counting and confluence measurements in the onboard software

Enhanced safety

Most operational functions can be controlled through software, eliminating the need to touch the instrument except to mount the slide and adjust the stage

Celleste Image Analysis Software option

Compatibility for advanced image processing and analysis, including 2D and 3D deconvolution and 3D visualization and analysis

Demo videos & customer stories

View more EVOS systems videos and demos under Resources.

Featured Images

11-slice image Z-stack of honeybee claw 


EVOS M5000 in color mode, 10x Apo objective.

Differentiated mouse embryonic stem cells


EVOS M5000. 10x objective.

HeLa cells with stained mitochondria


EVOS M5000. 40x Fluorite objective.

Human adenocarcinoma section


EVOS M5000 in color mode, 20X objective.

Section of rat intestine


Section of rat intestineEVOS M5000, RGB mode illumination. 10x objective.



View more M5000 images and movies in our Image Gallery and Video Gallery.





For Research Use Only. Not for use in diagnostic procedures.