CellInsight CX5 High-Content Screening (HCS) Platform
The Thermo Scientific™ CellInsight™ CX5 High-Content Screening (HCS) Platform is a compact, affordable screening system for researchers looking to increase scale and capacity in everyday use.
Increase your screening throughput with CellInsight CX5 Platform
With the compact and powerful CellInsight CX5 High-Content Screening Platform you can read and analyze a 96-well plate in under four minutes. Designed for researchers looking to increase their screening throughput, the platform offers common excitation wavelengths for fluorescence and white light illumination for colorimetric samples. The system is ready for full automation with 96-, 384-, or 1,536-well plates, or you can analyze your tissue sections one slide at a time to work up your next assay.
CellInsight CX5 system highlights
|Illumination||5-channel solid-state LED light engine|
|Camera||Photometrics X1 camera|
|Widefield||5-channel fluorescent imaging|
|Brightfield||White light brightfield imaging|
|Objectives||Single-position objective (range from 2x–20x, low and high NA)|
|Automation||Configured for fully automated plate handling and scanning|
|Software||HCS Studio™ software for integrated data collection and analysis|
BPAE cells imaged using the CellInsight CX5 platform and stained with DAPI (blue), Alexa Fluor™ 568 phalloidin (red), and goat anti–mouse IgG (H+L) secondary antibody, Alexa Fluor 488 conjugate (green).
White light brightfield image of a fixed tissue slice stained with Hematoxylin and Eosin imaged with the CellInsight CX5 platform.
Reagents optimized for high-content screening
Drawing on decades of experience in fluorescence imaging, Molecular Probes™ HCS products are developed using Thermo Scientific™ High-Content Platforms with special considerations for the high-throughput workflow and automated imaging:
- Cell and nuclear masks for automated demarcation
- Robust functional probes for cell health interrogation
- Flexible assay workflow for automated processing
- Broad fluorophore choice for easy multiplexing
- Validated on multiple imaging platforms
Other cell structure and segmentation tools
Clear identification of cell and nuclear boundaries in automated imaging requires fiducial markers that will label cellular structures robustly and reproducibly. Cell masks are designed specifically for automated imaging. Molecular Probes cell structure labels are widely used in microscope imaging and HCS.
Other cell health and cytotoxicity assays
We offer a diverse selection of imaging and microscopy assays for the analysis of cell viability, proliferation apoptosis, cell cycle and DNA repair, migration, adhesion and other cellular functions. Many of these assays have been validated specifically for HCS platforms.
Browse cell health and cytotoxicity assays by application:
Microplates and slides
Compatible with SBS standard microplates (6–1,536 well) and slides
Orbitor™ RS Microplate Mover
Increase throughput, storage capacity and operational flexibility with the Thermo Scientific™ Orbitor™ RS Microplate Mover—industrial-sized performance within a compact, benchtop mover.
The Orbitor RS is a high-speed microplate mover offering proven, reliable performance with totally flexible plate handling. Extensive vertical reach allows multiple stacked or high density instruments to be loaded in a small footprint, and a bi-directional telescoping arm provides superior reach, improved user safety and unlimited base rotations within a 360° workspace.
|Camera||Photometrics X1 CCD:
|Data management||Compatible with Thermo Scientific™ Store™ Image and Database Management Software|
|Dimensions||Height: 37.2 cm (14.6 in)
Width: 41.4 cm (16.3 in)
Depth: 59.4 cm (23.4 in)
|Image compatibility||JPG, BMP, GIF, PNG, TIF, C01, DIB|
|Light source||LED, solid-state five-color light engine used with provided filter sets offers the following excitation/emission capabilities (Ex/Em, in nm):
|Objectives||Standard (Olympus objectives):
|PC||Windows 7 Professional 64-Bit PC with 32GB (4x8GB) RAM, Intel™ Xeon™ Processor 3.7GHz Turbo, RAID I Controller with 1.8TB Hard Drive and 256 Boot Drive.
Includes Dell 24" High Resolution Widescreen Monitor or equivalent, keyboard and mouse.
|Software||Thermo Scientific™ HCS Studio™ 3.0 Cell Analysis Software|
|Weight||45 kg (99.2 lb)|
Thermo Fisher Cloud provides scientists with a secure place to store, share and analyze their data. Upload your files from anywhere you have access to the Internet by simply dragging them to the Cloud folder on your Windows™ desktop. Share the results with your colleagues to observe patterns, validate findings, and develop insights. And it’s not just for data files. Upload a presentation for an upcoming conference or a particularly useful how-to video.
- Elucidating the Mechanism of Cell Death Through the use of High Content Analysis
- Oxidative Stress Measurements Made Easy: CellROX™ Reagents with Simple Workflow for High Content Imaging
- High Content Cell Cycle Screening: Pairing FUCCI Technology with Thermo Scientific™ HCS Studio 2.0 Software and FCS Express Image Cytometry
- Using a High Content Analysis Database as a Repository for Data
- Multiplexed Mitosis and Apoptosis Analysis
- Monitoring Neurite Morphology and Synapse Formation in Primary Neurons for Neurotoxicity Assessments and Drug Screening
- Optimizing the high content analysis of cell morphology using multi-dimensional imaging on the Thermo Scientific ArrayScan XTI HCA Reader – Part 1 Stem Cell Colonies
For Research Use Only. Not for use in diagnostic procedures.