photo of the CellInsight CX7 High-Content Screening (HCS) Platform

The Thermo Scientific CellInsight CX7 High-Content Screening (HCS) Platform is an integrated system for all-around performance in screening and analysis.
 

Flexible imaging options with the CellInsight CX7 High-Content Screening Platform

With the CellInsight CX7 High-Content Screening Platform you have a choice of imaging modes to extract the information you need from your samples. Use the entire fluorescence spectrum to optimize your assay, and select either widefield or confocal optics for any channel. You also have 4-color brightfield options for colorimetric analysis of tissue sections. You can’t always predict what assays are coming next, but the CellInsight CX7 HCS Platform has you covered.

CellInsight CX7 system highlights

Illumination 7-channel solid-state LED light engine
Camera Photometrics X1 camera 
Widefield 7-channel fluorescent imaging
Brightfield 5-channel brightfield imaging
Confocal 7-channel confocal imaging
Objectives 3-position objective (range from 2x–40x, low and high NA)
Focus Software and laser-based autofocus for consistent scan times
Automation Configured for fully automated plate handling and scanning
Software HCS Studio software for integrated data collection and analysis
HeLa cells imaged using the CellInsight CX7 platform

HeLa cell image acquired using the Thermo Scientific™ CellInsight™ CX7 platform and stained with Invitrogen™ HCS NuclearMask™ Blue (blue), Invitrogen™ MitoTracker™ Orange (green), and Invitrogen™ Alexa Fluor™ 647 Phalloidin (red).

A549 cells imaged using the CellInsight CX7 platform

A549 cell image acquired using the Thermo Scientific™ CellInsight™ CX7 platform and stained with Invitrogen™ HCS CellMask™ Blue (blue), Invitrogen™ Alexa Fluor™ 488 Phalloidin (green), and Invitrogen™ Alexa Fluor™ 750 goat anti–mouse IgG secondary antibody (pink).

Three-color brightfield image of a fixed tissue slice imaged with the CellInsight CX7 platform.

Three-color brightfield image of fixed human breast cancer tissue stained with KI67-DAB and counterstained with hematoxylin and eosin and imaged with the Thermo Scientific™ CellInsight™ CX7 platform.

Reagents optimized for high-content screening

Drawing on decades of experience in fluorescence imaging, Invitrogen™ Molecular Probes™ HCS products are developed using Thermo Scientific High-Content Platforms with special considerations for the high-throughput workflow and automated imaging:

  • Cell and nuclear masks for automated demarcation
  • Robust functional probes for cell health interrogation
  • Flexible assay workflow for automated processing
  • Broad fluorophore choice for easy multiplexing
  • Validated on multiple imaging platforms

Select reagents for use with the CellInsight CX7

Other cell structure and segmentation tools

Clear identification of cell and nuclear boundaries in automated imaging requires fiducial markers that will label cellular structures robustly and reproducibly. Cell masks are designed specifically for automated imaging. Molecular Probes cell structure labels are widely used in microscope imaging and HCS.

See other reagents for cell structure

Other cell health and cytotoxicity assays

We offer a diverse selection of imaging and microscopy assays for the analysis of cell viability, proliferation apoptosis, cell cycle and DNA repair, migration, adhesion and other cellular functions. Many of these assays have been validated specifically for HCS platforms.

Browse cell health and cytotoxicity assays by application:

Microplates and slides

Compatible with SBS standard microplates (6–1,536 well) and slides

Automation

   

Orbitor RS Microplate Mover

Increase throughput, storage capacity and operational flexibility with the Thermo Scientific™ Orbitor™ RS Microplate Mover—industrial-sized performance within a compact, benchtop mover.

The Orbitor RS is a high-speed microplate mover offering proven, reliable performance with totally flexible plate handling. Extensive vertical reach allows multiple stacked or high density instruments to be loaded in a small footprint, and a bi-directional telescoping arm provides superior reach, improved user safety and unlimited base rotations within a 360° workspace.

 
Camera Photometrics X1 CCD:
  • High-resolution, thermoelectrically cooled to 0°C
  • 14-bit dynamic range
  • FOV 2208 x 2208 pixel array
  • 4.54 micron pixel size
  • 75% peak quantum efficiency (QE)
Data management Compatible with Thermo Scientific™ Store™ Image and Database Management Software
Dimensions 20 in. x 32 in. x 18 in. (50.8 cm x 81.3 cm x 45.7 cm)
Image compatability JPG, BMP, GIF, PNG, TIF, C01, DIB
Light source LED, solid-state 7-color light engine used with provided filter sets offers the following  excitation/emission capabilities (Ex/Em, in nm):
  • 386/440 (e.g., Alexa Fluor 350 dye)
  • 438/480 (e.g., Alexa Fluor 405 dye)
  • 485/521 (e.g., Alexa Fluor 488 dye)
  • 549/600 (e.g., Alexa Fluor 555 dye)
  • 560/607 (e.g., Alexa Fluor 594 dye)
  • 650/694 (e.g., Alexa Fluor 647 dye)
  • 740/810 (e.g., Alexa Fluor 750 dye)
Objectives Standard (Olympus™ objectives):
  • 10X (0.30NA)
  • 20X (0.4NA)
  • 20x (0.7 NA) Olympus objectives
  • 40x (0.6 NA) Olympus objectives
Optional (Olympus objectives):
  • 2x (0.08NA)
  • 4x (0.16NA)
PC Windows 7 Professional 64-Bit PC with 32GB (4x8GB) RAM, Intel® Xeon® Processor 3.7GHz Turbo, RAID I Controller with 1.8TB Hard Drive and 256 Boot Drive.
Includes Dell 24" High Resolution Widescreen Monitor or equivalent, keyboard and mouse.
Software Thermo Scientific™ HCS Studio™ 3.0 Cell Analysis Software
Wattage 300 W
Weight 150 lb (68 kg)