Construct high quality full length cDNA libraries using Invitrogen™ SuperScript™ reverse transcriptase combined with Invitrogen™ Gateway™ cloning for uncut libraries (no use of restriction digestion). Or use standard restriction enzyme cloning.

CloneMiner II cDNA Library Construction Kit

The Invitrogen™ CloneMiner™ II cDNA Library Construction Kit is a second generation CloneMiner kit that enables rapid construction of highly representative cDNA libraries without restriction enzyme cloning. This innovative library construction technology combines Invitrogen™ SuperScript III Reverse Transcriptase with Gateway cloning technology, resulting in the discovery of previously unobtainable, full-length clones. The kit also avoids the use of time-consuming, inefficient ligation reactions, making library construction faster and offering better representation.

SuperScript Plasmid System for cDNA Synthesis with Gateway Technology

The Invitrogen™ SuperScript™ Plasmid System is designed for synthesis of double-stranded cDNA from a purified mRNA population. The cDNA is suitable for directional cloning into the pSPORT1 vector or the pCMV•SPORT6 plasmid vector for subsequent generation of cDNA libraries and use of Gateway Technology.