Explore the “Getting Started” and “Troubleshooting” sections for solutions to top inquiries and common problems. Browse through the “Guides and Tools” section to access comprehensive product-related support resources.
- Should I run any internal controls with your ELISA kits? Where can I get internal controls?
- What’s the difference between the chromogen blank and the zero standard in your ELISA kits? Is it necessary to run both of these types of blanks?
- How does the IgG Subclass Human ELISA Kit (Cat. No. 99-1000) work?
- What can you tell me about the Human IgG Subclass Standard included in the IgG Subclass Human ELISA Kit (Cat. No. 99-1000)?
- What is the difference between your total and phosphospecific ELISA kits?
- My sample lysates were made in RIPA buffer. Can I test these samples with your ELISA kits?
- I ran out of the streptavidin-HRP that came in your ELISA (or Antibody Pair) kit. Can I get more?
- I bought one of your Antibody Pair ELISA kits, but there were no buffers included. How should I set up and run the ELISA?
- The A450 values that I got for the standard curve in your ELISA kit are lower than the example values shown in the product manual. Why?
- After I added the chromogen reagent to the plate, I incubated the ELISA as suggested in the manual, but the A450 of the highest standard was higher than what my plate reader can read. What should I do?
Guides and Tools
- Overview: Immunoassays
- Overview: ELISA (Enzyme-Linked Immunosorbent Assay)
- Overview: ELISA Reagents and Accessories
- Overview: ELISA Kits
- Overview: Antibody Pair Kits
- Overview: Protein Quantitation by ELISA
- Learning Center: Antibodies
- Antibodies Resource Library (useful technical, application, and other learning resources including application notes and methods)
- Learning Center: Protein Biology
- Protein Biology Resource Library (useful technical, application, and other learning resources including application notes and methods)
- Application Note: Proper Sample Handling for Immunoassays
- Guide: Biomarker Quantitation Assay Guide
- Guide: Protein Analysis Handbook
- Protocol: General Guidelines for ELISA
- References: ELISA Kit Literature References
- Selection: ELISA Enzyme Substrates Selection Guide
- Video: Novex ELISA Kits for Protein Analysis
- Video: Using Novex Antibody Pairs to Make an ELISA
- Video: Novex Multiplex Immunoassay Tips and Tricks
- White Paper: Multiplex Assays on the Luminex Platform
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