AlgiMatrix® 3D Cell Culture System
AlgiMatrix™ 3D Culture System is the first user friendly, animal-free bioscaffold available for the development of higher-fidelity cell culture models that are more predictive of disease states and drug responses.
AlgiMatrix® Product Information
AlgiMatrix® sponge is formulated using pharmaceutical-grade raw material from brown seaweed. Its pure, chemically defined, and nontoxic macro porous structure improved lot-to-lot consistency, superior cell loading and excellent nutrient delivery, without damage to cells. The AlgiMatrix® sponge is biodegradable and is stable at room temperature.
The AlgiMatrix® 3D Culture System is suitable for a broad range of procedures; including Multicellular tumor spheroid assays (MCTS), hepatocyte and cardiomyocyte organogenesis studies, co-culture studies, high-throughput drug screening assays, and embryonic stem cell 3D differentiation. This broad applicability fulfills a critical need for cell-based screening, drug discovery, and in vitro human cell therapy research.
To assess cell viability and proliferation, use Alamar Blue™ directly on spheroids within the AlgiMatrix™ 3D Culture System according to the manufacturer’s instructions.
To perform assays on isolated spheroids, dissolve AlgiMatrix™ 3D Culture System with iso-osmolar trisodium citrate.
To isolate individual cells, isolate spheroids as described above and add 2 ml TrypLE™ Select or Trypsin-EDTA to a 15 ml centrifuge tube with spheroids, place at 37°C and triturate several times for 15-20 minutes. Proceed with downstream assay.
- For antibody staining, embed AlgiMatrix™ 3D Culture System – containing spheroids in paraffin according to your regular protocol before sectioning.