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Invitrogen
Mouse IgA Monoclonal Antibody (mA-6E1), PE, eBioscience™
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FIGURE: 1 / 15
Mouse IgA Antibody (12-4204-82) in Flow
Nature communications 2022 -
Rad52 deletion ablates in vivo Smu-sigmadelta DNA recombination and reduces IgD production. Rad52 +/+ and Rad52 -/- mice were immunized with OVA in alum i.p. a Recombined Smu-sigmadelta, Smu-Sgamma1, and Smu-Salpha DNAs in spleen, mesenteric lymph nodes (MLNs), and Peyer's patches (PPs) B cells, as analyzed by nested PCR using forward Imu and reverse Cdelta, Sgamma1, or Salpha primers, respectively, followe... View More
Product Details
12-4204-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Bacteria,
Human,
Mouse
Host/Isotype
Rat
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
mA-6E1
Conjugate
Excitation/Emission Max
565/576 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The mA-6E1 monoclonal antibody reacts with the heavy chain of mouse IgA. It does not react with other isotypes of antibody, including IgD, IgG or IgM, or with other species isotypes. IgA, which is mainly found as a dimer through association of the J chain, is found in mucous secretions, but not in plasma. Fluorochrome conjugated mA-6E1 is used as a detection secondary for mouse IgA primary antibodies.
Applications Reported: This mA-6E1 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This mA-6E1 antibody has been tested by flow cytometric analysis as a second step to detect mouse IgA primary antibodies. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Mouse IgA is an immunoglobulin A antibody that is produced by B cells in response to an antigen. It is a crucial component of the mucosal immune system, which protects the body from pathogens that enter through mucosal surfaces such as the respiratory, gastrointestinal, and urogenital tracts. Mouse IgA is composed of two heavy chains and two light chains, and it is the most abundant immunoglobulin in mucosal secretions. It plays a vital role in neutralizing pathogens and preventing their attachment to mucosal surfaces. Mouse IgA is widely used in research and diagnostic applications, including ELISA, Western blotting, and immunohistochemistry.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
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