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Invitrogen

CCL2 (MCP-1) Monoclonal Antibody (2H5), PE, eBioscience™

1 Published Figure
2 References
View all (62) CCL2 (MCP-1) antibodies

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Product Details

12-7096-82

Applications
Tested Dilution
Publications

Flow Cytometry (Flow)

0.25 µg/test
View 2 publications 2 publications
Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Armenian hamster / IgG

Recommended Isotype Control

Armenian Hamster IgG Isotype Control (eBio299Arm), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

2H5

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra spectra

Form

Liquid

Concentration

0.2 mg/mL

Amount

100 µg

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_466171

Product Specific Information

Description: The 2H5 monoclonal antibody reacts with mouse, rat, and human monocyte chemoattractant protein-1 (MCP-1), also known as CCL2 and MCAF.

Applications Reported:The 2H5 antibody has been reported for use in ELISA, intracellular staining for flow cytometric analysis, and neutralization. (Please use Functional Grade purified 2H5 (Product # 16-7096-81) in functional assays).

Applications Tested: This 2H5 antibody is tested by flow cytometric analysis of fixed permabilized LPS-stimulated mouse thioglycolate-elicited peritoneal exudate cells. Has been tested by flow cytometric analysis of fixed permabilized LPS-stimulated mouse thioglycolate-elicited peritoneal exudate cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The protein encoded by this gene is structurally related to the CXC subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Bioinformatics

Protein Aliases: Ccl2; chemokine (C-C motif) ligand 2; chemotactic and activating factor precursor; Genbank Staff Annotation: The small inducible cytokine A2 (SWISS-PROT ACC P13500) is encoded in this mRNA, although it is interrupted and partial, perhaps indicating that this CDS is not the true product of the gamma.1 gene.; H-MCP-1; immediate-early serum-responsive JE protein; immediate-early serum-responsive protein JE; JE; MGC9434; monocyte chemoattractant protein 1; monocyte chemoattractant protein-1; monocyte chemotactic and activating factor; monocyte chemotactic protein; monocyte chemotactic protein 1; monocyte secretory protein JE; Mutations: 171:OK; platelet-derived growth factor-inducible protein JE; RP23-350G1.3; small inducible cytokine A2; small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig-je); small inducible cytokine subfamily A (Cys-Cys), member 2; small inducible gene JE; small-inducible cytokine A2; unnamed protein product

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Gene Aliases: GDCF-2; HC11; HSMCR30; JE; MCAF; MCP-1; MCP1; SCYA2; Sigje; SMC-CF

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UniProt ID: (Human) P13500, (Mouse) P10148, (Rat) P14844

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Entrez Gene ID: (Human) 6347, (Mouse) 20296, (Rat) 24770

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Function(s)
protein kinase activity receptor binding protein binding chemokine activity CCR2 chemokine receptor binding chemoattractant activity chemokine receptor binding CCR chemokine receptor binding G-protein coupled receptor binding cytokine activity heparin binding
Process(es)
angiogenesis response to hypoxia positive regulation of leukocyte mediated cytotoxicity positive regulation of endothelial cell proliferation leukocyte migration involved in inflammatory response chronic inflammatory response monocyte chemotaxis positive regulation of leukocyte migration positive regulation of cellular extravasation cellular calcium ion homeostasis inflammatory response immune response cytoskeleton organization transforming growth factor beta receptor signaling pathway glial cell migration regulation of cell shape response to xenobiotic stimulus response to wounding response to mechanical stimulus response to bacterium response to gamma radiation regulation of vascular endothelial growth factor production positive regulation of gene expression positive regulation of macrophage chemotaxis response to activity negative regulation of angiogenesis cytokine-mediated signaling pathway sensory perception of pain positive regulation of cell-cell adhesion positive regulation of cell migration neutrophil chemotaxis organ regeneration response to lipopolysaccharide response to progesterone positive regulation of tumor necrosis factor production cellular response to insulin stimulus positive regulation of collagen biosynthetic process response to vitamin B3 negative regulation of glial cell apoptotic process response to tumor necrosis factor helper T cell extravasation monocyte homeostasis response to isolation stress cellular response to macrophage colony-stimulating factor stimulus cellular response to platelet-derived growth factor stimulus chemokine (C-C motif) ligand 2 signaling pathway response to amino acid negative regulation of neuron apoptotic process astrocyte cell migration response to ethanol positive regulation of cell adhesion vascular endothelial growth factor receptor signaling pathway eosinophil chemotaxis macrophage chemotaxis lymphocyte chemotaxis positive regulation of synaptic transmission positive regulation of T cell activation positive chemotaxis response to glucocorticoid positive regulation of synaptic transmission, glutamatergic mammary gland involution maternal process involved in female pregnancy maternal process involved in parturition antimicrobial humoral immune response mediated by antimicrobial peptide chemokine-mediated signaling pathway cellular response to lipopolysaccharide cellular response to retinoic acid cellular response to ATP cellular response to glucose stimulus cellular response to interferon-gamma cellular response to interleukin-1 cellular response to interleukin-6 cellular response to tumor necrosis factor cellular response to estradiol stimulus cellular response to fatty acid cellular response to lipoprotein particle stimulus cellular response to high density lipoprotein particle stimulus cellular response to xenobiotic stimulus cellular response to dexamethasone stimulus positive regulation of monocyte chemotaxis positive regulation of immune complex clearance by monocytes and macrophages positive regulation of calcium ion import positive regulation of wound healing positive regulation of protein targeting to membrane energy homeostasis positive regulation of glutamate receptor signaling pathway response to cyclosporin A negative regulation of vascular endothelial cell proliferation negative regulation of G1/S transition of mitotic cell cycle positive regulation of endothelial cell apoptotic process positive regulation of apoptotic cell clearance negative regulation of natural killer cell chemotaxis positive regulation of cytosolic calcium ion concentration osteoclast differentiation chemokinesis cellular response to fibroblast growth factor stimulus protein phosphorylation chemotaxis humoral immune response cell adhesion signal transduction cell surface receptor signaling pathway G-protein coupled receptor signaling pathway G-protein coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger JAK-STAT cascade organ morphogenesis viral genome replication cellular homeostasis
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