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Invitrogen
CCL2 (MCP-1) Monoclonal Antibody (2H5), Functional Grade, eBioscience™
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FIGURE: 1 / 6
CCL2 (MCP-1) Antibody (16-7096-81) in ICC/IF
Journal of extracellular vesicles 2021 -
8 FIGURE CRT mediates efferocytosis of MSC-derived apoVs to modulate T2D liver macrophages in vivo. (a) Representative immunofluorescent (IF) staining images of F4/80 (green) and CD11b (green) in the liver, counterstained by Hoechst (blue). DIO, mice with diet-induced obesity; si-NC-apoV, DIO mice treated by apoVs derived from MSCs transfected by siRNA-negative control; si- CRT -apoV, DIO mice treated by ap... View More
Product Details
16-7096-81
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human,
Mouse
Host/Isotype
Armenian hamster
/ IgG
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
2H5
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
50 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
no preservative
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The 2H5 monoclonal antibody reacts with mouse, rat, and human monocyte chemoattractant protein-1 (MCP-1), also known as CCL2 and MCAF.
Applications Reported: The 2H5 antibody has been reported for use in ELISA, intracellular staining for flow cytometric analysis, and cytokine neutralization. .
Applications Tested: The Functional Grade purified 2H5 antibody has been tested by ELISA for verification of reactivity and by LAL assay for verification of low endotoxin. The 2H5 antibody has been published for use in vitro and in vivo for neutralization of MCP-1 activity.
Mouse MCP-1 ELISA: The biotinylated 2H5 antibody has been tested as the detection antibody in a sandwich ELISA for analysis of mouse MCP-1 in combination with the affinity purified 4E2/MCP (14-7091) antibody for capture and recombinant mouse MCP-1 as the standard. A suitable range of concentrations of this antibody for ELISA detection is 0.5-2.0 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 2000 pg/mL - 15 pg/mL should be included in each ELISA plate.
Human MCP-1 ELISA: The biotinylated 2H5 antibody has been tested as the detection antibody in a sandwich ELISA for analysis of human MCP-1 in combination with the affinity purified 5D3-F7 (14-7099) antibody for capture and recombinant human MCP-1 as the standard. A suitable range of concentrations of this antibody for ELISA detection is 0.5-2.0 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 2000 pg/mL - 15 pg/mL should be included in each ELISA plate.
Storage and handling: Use in a sterile environment.
Filtration: 0.2 µm post-manufacturing filtered.
Purity: Greater than 90%, as determined by SDS-PAGE.
Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.
Aggregation: Less than 10%, as determined by HPLC.
Target Information
This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The protein encoded by this gene is structurally related to the CXC subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: C-C motif chemokine 2; chemokine (C-C motif) ligand 2; chemotactic and activating factor precursor; Genbank Staff Annotation: The small inducible cytokine A2 (SWISS-PROT ACC P13500) is encoded in this mRNA, although it is interrupted and partial, perhaps indicating that this CDS is not the true product of the gamma.1 gene.; H-MCP-1; HC11; immediate-early serum-responsive JE protein; Immediate-early serum-responsive protein JE; MCAF; MCP-1; MGC9434; Monocyte chemoattractant protein 1; monocyte chemoattractant protein-1; Monocyte chemotactic and activating factor; monocyte chemotactic protein; Monocyte chemotactic protein 1; Monocyte secretory protein JE; Mutations: 171:OK; Platelet-derived growth factor-inducible protein JE; RP23-350G1.3; small inducible cytokine A2; small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig-je); small inducible cytokine subfamily A (Cys-Cys), member 2; small inducible gene JE; Small-inducible cytokine A2; unnamed protein product
Gene Aliases: AI323594; CCL2; GDCF-2; HC11; HSMCR30; JE; MCAF; MCP-1; MCP1; SCYA2; Sigje; SMC-CF
UniProt ID: (Human) P13500, (Rat) P14844, (Mouse) P10148
Entrez Gene ID: (Human) 6347, (Rat) 24770, (Mouse) 20296
angiogenesis
monocyte chemotaxis
protein phosphorylation
chemotaxis
inflammatory response
immune response
humoral immune response
cytoskeleton organization
cell adhesion
signal transduction
cell surface receptor signaling pathway
G-protein coupled receptor signaling pathway
G-protein coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger
JAK-STAT cascade
regulation of cell shape
response to bacterium
organ morphogenesis
positive regulation of gene expression
positive regulation of macrophage chemotaxis
viral genome replication
cytokine-mediated signaling pathway
sensory perception of pain
cellular homeostasis
positive regulation of cell migration
negative regulation of glial cell apoptotic process
helper T cell extravasation
chemokine (C-C motif) ligand 2 signaling pathway
negative regulation of neuron apoptotic process
astrocyte cell migration
cellular response to fibroblast growth factor stimulus
eosinophil chemotaxis
macrophage chemotaxis
positive regulation of T cell activation
positive chemotaxis
positive regulation of synaptic transmission, glutamatergic
antimicrobial humoral immune response mediated by antimicrobial peptide
chemokine-mediated signaling pathway
cellular response to lipopolysaccharide
cellular response to interferon-gamma
cellular response to interleukin-1
cellular response to tumor necrosis factor
positive regulation of calcium ion import
positive regulation of glutamate receptor signaling pathway
negative regulation of vascular endothelial cell proliferation
negative regulation of G1/S transition of mitotic cell cycle
positive regulation of endothelial cell apoptotic process
positive regulation of apoptotic cell clearance
negative regulation of natural killer cell chemotaxis
MAPK cascade
response to hypoxia
positive regulation of leukocyte mediated cytotoxicity
positive regulation of endothelial cell proliferation
leukocyte migration involved in inflammatory response
positive regulation of leukocyte migration
positive regulation of cellular extravasation
cellular calcium ion homeostasis
transforming growth factor beta receptor signaling pathway
aging
glial cell migration
response to heat
response to wounding
response to mechanical stimulus
response to gamma radiation
regulation of vascular endothelial growth factor production
response to activity
negative regulation of angiogenesis
positive regulation of cell-cell adhesion
neutrophil chemotaxis
organ regeneration
lipopolysaccharide-mediated signaling pathway
response to lipopolysaccharide
response to progesterone
positive regulation of tumor necrosis factor production
cellular response to insulin stimulus
positive regulation of collagen biosynthetic process
response to vitamin B3
cellular response to drug
cellular response to macrophage colony-stimulating factor stimulus
cellular response to platelet-derived growth factor stimulus
chemokinesis
response to drug
response to amino acid
protein kinase B signaling
response to ethanol
positive regulation of cell adhesion
response to antibiotic
vascular endothelial growth factor receptor signaling pathway
lymphocyte chemotaxis
positive regulation of synaptic transmission
response to glucocorticoid
positive regulation of nitric-oxide synthase biosynthetic process
maternal process involved in female pregnancy
maternal process involved in parturition
cellular response to retinoic acid
cellular response to ATP
cellular response to interleukin-6
cellular response to lipoprotein particle stimulus
cellular response to high density lipoprotein particle stimulus
cellular response to organic cyclic compound
cellular response to dexamethasone stimulus
positive regulation of monocyte chemotaxis
positive regulation of immune complex clearance by monocytes and macrophages
positive regulation of protein targeting to membrane
monocyte homeostasis
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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