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Invitrogen

CCL2 (MCP-1) Monoclonal Antibody (2H5), Biotin, eBioscience™

9 Published Figures
11 References
View all (63) CCL2 (MCP-1) antibodies

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Product Details

13-7096-85

Applications
Tested Dilution
Publications

Western Blot (WB)

-
View 1 publication 1 publication

Immunohistochemistry (IHC)

-
View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

-
View 1 publication 1 publication

Flow Cytometry (Flow)

Assay-dependent
View 1 publication 1 publication

ELISA (ELISA)

0.5-2 µg/mL
View 6 publications 6 publications

Neutralization (Neu)

-
View 1 publication 1 publication
Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Armenian hamster / IgG

Recommended Isotype Control

Armenian Hamster IgG Isotype Control (eBio299Arm), Biotin, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

2H5

Conjugate

Biotin Biotin Biotin

Form

Liquid

Concentration

0.5 mg/mL

Amount

500 µg

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_466915

Product Specific Information

Description: The 2H5 monoclonal antibody reacts with mouse, rat, and human monocyte chemoattractant protein-1 (MCP-1), also known as CCL2 and MCAF.

Applications Reported: The 2H5 antibody has been reported for use in ELISA, intracellular staining for flow cytometric analysis, and cytokine neutralization. (Please use Functional Grade purified 2H5, Product # 16-7096, in functional assays).

Applications Tested: Mouse MCP-1 ELISA: The biotinylated 2H5 antibody has been tested as the detection antibody in a sandwich ELISA for analysis of mouse MCP-1 in combination with the affinity purified 4E2/MCP (Product # 14-7091-81) antibody for capture and recombinant mouse MCP-1 as the standard. A suitable range of concentrations of this antibody for ELISA detection is 0.5-2.0 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 2000 pg/mL - 15 pg/mL should be included in each ELISA plate.

Human MCP-1 ELISA: The biotinylated 2H5 antibody has been tested as the detection antibody in a sandwich ELISA for analysis of human MCP-1 in combination with the affinity purified 5D3-F7 (Product # 14-7099-81) antibody for capture and recombinant human MCP-1 as the standard. A suitable range of concentrations of this antibody for ELISA detection is 0.5-2.0 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 2000 pg/mL - 15 pg/mL should be included in each ELISA plate.

The Functional Grade purified 2H5 antibody has been tested for neutralization of MCP-1 bioactivity.The fluorochrome-conjugated 2H5 antibody has been tested for intracellular staining and flow cytometric analysis of mouse, rat, and human cells and can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The protein encoded by this gene is structurally related to the CXC subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: C-C motif chemokine 2; chemokine (C-C motif) ligand 2; chemotactic and activating factor precursor; Genbank Staff Annotation: The small inducible cytokine A2 (SWISS-PROT ACC P13500) is encoded in this mRNA, although it is interrupted and partial, perhaps indicating that this CDS is not the true product of the gamma.1 gene.; H-MCP-1; HC11; immediate-early serum-responsive JE protein; Immediate-early serum-responsive protein JE; MCAF; MCP-1; MGC9434; Monocyte chemoattractant protein 1; monocyte chemoattractant protein-1; Monocyte chemotactic and activating factor; monocyte chemotactic protein; Monocyte chemotactic protein 1; Monocyte secretory protein JE; Mutations: 171:OK; Platelet-derived growth factor-inducible protein JE; RP23-350G1.3; small inducible cytokine A2; small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig-je); small inducible cytokine subfamily A (Cys-Cys), member 2; small inducible gene JE; Small-inducible cytokine A2; unnamed protein product

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Gene Aliases: AI323594; CCL2; GDCF-2; HC11; HSMCR30; JE; MCAF; MCP-1; MCP1; SCYA2; Sigje; SMC-CF

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UniProt ID: (Human) P13500, (Rat) P14844, (Mouse) P10148

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Entrez Gene ID: (Human) 6347, (Rat) 24770, (Mouse) 20296

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Function(s)
protein kinase activity receptor binding cytokine activity protein binding chemokine activity CCR2 chemokine receptor binding chemoattractant activity CCR chemokine receptor binding G-protein coupled receptor binding heparin binding cytokine intercellular signal molecule
Process(es)
angiogenesis monocyte chemotaxis protein phosphorylation chemotaxis inflammatory response immune response humoral immune response cytoskeleton organization cell adhesion signal transduction cell surface receptor signaling pathway G-protein coupled receptor signaling pathway G-protein coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger JAK-STAT cascade regulation of cell shape response to bacterium organ morphogenesis positive regulation of gene expression positive regulation of macrophage chemotaxis viral genome replication cytokine-mediated signaling pathway sensory perception of pain cellular homeostasis positive regulation of cell migration negative regulation of glial cell apoptotic process helper T cell extravasation chemokine (C-C motif) ligand 2 signaling pathway negative regulation of neuron apoptotic process astrocyte cell migration cellular response to fibroblast growth factor stimulus eosinophil chemotaxis macrophage chemotaxis positive regulation of T cell activation positive chemotaxis positive regulation of synaptic transmission, glutamatergic antimicrobial humoral immune response mediated by antimicrobial peptide chemokine-mediated signaling pathway cellular response to lipopolysaccharide cellular response to interferon-gamma cellular response to interleukin-1 cellular response to tumor necrosis factor positive regulation of calcium ion import positive regulation of glutamate receptor signaling pathway negative regulation of vascular endothelial cell proliferation negative regulation of G1/S transition of mitotic cell cycle positive regulation of endothelial cell apoptotic process positive regulation of apoptotic cell clearance negative regulation of natural killer cell chemotaxis MAPK cascade response to hypoxia positive regulation of leukocyte mediated cytotoxicity positive regulation of endothelial cell proliferation leukocyte migration involved in inflammatory response positive regulation of leukocyte migration positive regulation of cellular extravasation cellular calcium ion homeostasis transforming growth factor beta receptor signaling pathway aging glial cell migration response to heat response to wounding response to mechanical stimulus response to gamma radiation regulation of vascular endothelial growth factor production response to activity negative regulation of angiogenesis positive regulation of cell-cell adhesion neutrophil chemotaxis organ regeneration lipopolysaccharide-mediated signaling pathway response to lipopolysaccharide response to progesterone positive regulation of tumor necrosis factor production cellular response to insulin stimulus positive regulation of collagen biosynthetic process response to vitamin B3 cellular response to drug cellular response to macrophage colony-stimulating factor stimulus cellular response to platelet-derived growth factor stimulus chemokinesis response to drug response to amino acid protein kinase B signaling response to ethanol positive regulation of cell adhesion response to antibiotic vascular endothelial growth factor receptor signaling pathway lymphocyte chemotaxis positive regulation of synaptic transmission response to glucocorticoid positive regulation of nitric-oxide synthase biosynthetic process maternal process involved in female pregnancy maternal process involved in parturition cellular response to retinoic acid cellular response to ATP cellular response to interleukin-6 cellular response to lipoprotein particle stimulus cellular response to high density lipoprotein particle stimulus cellular response to organic cyclic compound cellular response to dexamethasone stimulus positive regulation of monocyte chemotaxis positive regulation of immune complex clearance by monocytes and macrophages positive regulation of protein targeting to membrane monocyte homeostasis
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