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Invitrogen
TRA-1-60 (Podocalyxin) Monoclonal Antibody (TRA-1-60), eBioscience™
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FIGURE: 1 / 15
TRA-1-60 (Podocalyxin) Antibody (14-8863-95) in Flow
Staining of 2102Ep human embryonal carcinoma cell line with 0.25 µg of Mouse IgM Isotype Control Purified (Product # 14-4752-82) (open histogram) or 0.25 µg of Anti-Human TRA-1-60 Purified (filled histogram) followed by Anti-Mouse IgM PE (Product # 12-5890-82).Total viable cells were used for analysis.
Product Details
14-8863-95
Applications
Tested Dilution
Publications
Product Specifications
Published species
Human,
Non-human primate,
Rhesus monkey
Host/Isotype
Mouse
/ IgM
Class
Monoclonal
Type
Antibody
Clone
TRA-1-60
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
10 mg
Storage conditions
4°C
Shipping conditions
Wet ice
RRID
Product Specific Information
Description: The TRA-1-60 antibody recognizes a protein expressed on undifferentiated human embryonic stem cells (ES), embyronal carcinoma cells (EC), and embryonic germ cells (EG). Like other stem cell specific markers, the epitope recognized by the TRA-1-60 antibody is lost upon cell differentiation. Contrary to early reports that the TRA-1-60 epitope can be destroyed by neuraminidase digestion, new strudies have shown that TRA-1-60 recognizes a neurominidase resistent antigen. The TRA-1-60 antibody is known to specifically recognize a carbohydrate epitope on a keratan sulfated glycoprotein recently identified as podocalyxin, a member of the CD34-related family of sialomucins. Podocalyxin is a transmembrane glycoprotein originally identified on epithelial glomerular cells known as podocytes, and the protein has also been implicated in the development of aggressiveness in a variety of cancers, including breast and prostate cancer.
Applications Reported: This TRA-1-60 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, immunoblotting, and immunofluorescence.
Applications Tested: This TRA-1-60 antibody has been tested by flow cytometric analysis of the human embryonal carcinoma (EC) line 2102Ep. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. This antibody has also been tested by immunoblotting of 2102Ep cell extracts, yielding a band of approximately 200-250 kDa (reducing and non-reducing conditions). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
TRA-1-60 is a cell surface antigen, expressed along with SSEA-3, SSEA-4 and TRA-1-81 in human embryonic stem cells, embryonal carcinoma cells and induced pluripotent stem cells (iPS). These surface markers are lost during the differentiation process. In contrast, SSEA-1 is absent in undifferentiated human stem cells but is present on the cell surface after retinoic acid mediated differentiation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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