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Ly-6G Monoclonal Antibody (1A8-Ly6g), Functional Grade, eBioscience™
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FIGURE: 1 / 32
Ly-6G Antibody (16-9668-025) in Flow
C57BL/6 mouse bone marrow cells were stained with Ly-6C Monoclonal Antibody, APC (Product # 17-5932-82) and 0.125 µg of Rat IgG2a kappa Isotype Control (Product # 14-4321-82) (left) or 0.125 µg of Ly-6G Monoclonal Antibody, Functional Grade (right) followed by IgG2a Monoclonal Antibody, PE (Product # 12-4817-82). Total viable cells were used for analysis, as determined by 7-AAD (Product # 00-6993-50).
Product Details
16-9668-025
Applications
Tested Dilution
Publications
Product Specifications
Published species
Mouse,
Virus
Host/Isotype
Rat
/ IgG2a, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
1A8-Ly6g
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
25 mg
Storage conditions
4°C
Shipping conditions
Wet ice
RRID
Product Specific Information
Description: The monoclonal antibody 1A8-Ly6g reacts with mouse Ly-6G, which is also known as Gr-1. Ly-6G is a 25-kDa GPI protein expressed exclusively by neutrophils. Studies suggest an important role in neutrophil infiltration, recruitment and migration. Ly-6G associates with B2 integrins CD11a and CD11b and can attenuate B2 integrin expression and function. The ligand for Ly-6G is currently unknown.
Unlike the RB6-8C5 antibody, 1A8-Ly6g is reported to only bind to Ly-6G found on neutrophils. Moreover, 1A8-Ly6g has been used to deplete neutrophils and reported to have antagonist activity.
Applications Reported: This 1A8-Ly6g antibody has been reported for use in flow cytometric analysis and depletion of neutrophils. Moreover, this antibody has been reported to have antagonist activity. (Fluorochrome-conjugated 1A8-Ly6g is recommended for use in flow cytometry).
Applications Tested: This 1A8-Ly6g antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Storage and handling: Use in a sterile environment.
Filtration: 0.2 µm post-manufacturing filtered.
Purity: Greater than 90%, as determined by SDS-PAGE.
Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.
Aggregation: Less than 10%, as determined by HPLC.
Target Information
Ly6G (Lymphocyte antigen 6 complex locus G6D) is a component of the myeloid differentiation antigen Gr-1, together with Ly6C. The Ly6G protein has a molecular weight of approximately 21-25 kDa and is expressed by myeloid derived cells in the bone marrow. Ly6G is a good marker for detection of peripheral neutrophils, monocytes and granulocytes. Expression of Gr-1 in bone marrow correlates with granulocyte differentiation and maturation. The physiological role of Ly6G remains unclear but studies have shown its involvement in anti-tumor response, and studies with neutrophils suggest that Ly6G ligation may have an inhibitory effect on local immune responses.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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