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Rockland
NAG-1 Monoclonal Antibody (23B3.D2.H5)
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FIGURE: 1 / 1
NAG-1 Antibody (200-301-B07) in WB
Western blot using Rocklands anti-NAG-1 monoclonal antibody. The blot shows detection of recombinant NAG-1 protein present in Pichia pastoris whole cell lysates: lane 1 - yeast cell lysate expressing NAG-1 H variant with SUMO expression tag at 36 kDa; lane 2 - yeast cell lysate expressing NAG-1 D variant with SUMO expression tag at 36 kDa; lane 3 - yeast cell lysate expressing NAG-1 H variant; and lane 4 - yeast cell lysate expressing NAG-1 D variant. Recombinant NAG-1 proteins without SUMO correspond to monomer (15 kDa) and dimer (30 kDa... View More
Product Details
200-301-B07
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
23B3.D2.H5
Immunogen
This Protein A purified antibody was prepared by repeated immunizations with a synthetic peptide corresponding to a region near the carboxy terminal end of human NAG-1 protein. A residue of cysteine was added to facilitate coupling to KLH.
Conjugate
Form
Liquid
Concentration
1.13 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
Contains
0.01% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
This antibody reacts with the C-terminus of endogenous NAG-1 protein from human tissues. A BLAST analysis suggests reactivity with NAG-1 from chimpanzee and macaque based on a 100% homology. Partial reactivity is expected against rat based on an 86% homology with the immunizing sequence. Cross-reactivity with NAG-1 from other sources has not been determined.
Target Information
GDF-15 belongs to the TGF-beta cytokine family, whose members play an important role during prenatal development and postnatal growth, and the remodeling and maintenance of a variety of tissues and organs. GDF-15 is expressed predominantly in the placenta and, to a much lesser extent, in various other tissues. The presence of GDF-15 in amniotic fluid and its elevated levels in the sera of pregnant women suggest GDF-15's involvement in gestation and embryonic development. GDF-15 generally exerts tumor suppressive activities and is one of the predominant factors produced and secreted in response to activation of the p53 pathway. Interestingly, the serum level of GDF-15 is positively correlated with neoplastic progression of several tumor types, including certain colorectal, pancreatic, and prostate cancers.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: GDF-15; Growth/differentiation factor 15; Macrophage inhibitory cytokine 1; MIC-1; NAG-1; NAG1; non-steroidal anti-inflammatory drug-activated gene-1; NRG-1; NSAID (nonsteroidal anti-inflammatory drug)-activated protein 1; NSAID-activated gene 1 protein; NSAID-regulated gene 1 protein; PDF; Placental bone morphogenetic protein; Placental TGF-beta; Prostate differentiation factor; PTGF-beta; TGF-b superfamily cytokine; unnamed protein product
Gene Aliases: GDF-15; GDF15; HG; MIC-1; MIC1; NAG-1; PDF; PLAB; PTGFB
UniProt ID: (Human) Q99988
Entrez Gene ID: (Human) 9518
reduction of food intake in response to dietary excess
negative regulation of leukocyte migration
signal transduction
transmembrane receptor protein serine/threonine kinase signaling pathway
transforming growth factor beta receptor signaling pathway
cell-cell signaling
negative regulation of transforming growth factor beta receptor signaling pathway
negative regulation of appetite
negative regulation of multicellular organism growth
positive regulation of MAPK cascade
positive regulation of fatty acid oxidation
positive regulation of protein kinase B signaling
negative regulation of SMAD protein import into nucleus
negative regulation of growth hormone receptor signaling pathway
cellular response to chemical stress
energy homeostasis
GDF15-GFRAL signaling pathway
response to metformin
positive regulation of myoblast fusion
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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