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Invitrogen

PLZF Monoclonal Antibody (9E12), PE-Cyanine7, eBioscience™

1 Published Figure
2 References
View all (20) PLZF antibodies

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Product Details

25-9322-80

Applications
Tested Dilution
Publications

Flow Cytometry (Flow)

0.25 µg/test
View 2 publications 2 publications
Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Armenian hamster / IgG

Recommended Isotype Control

Armenian Hamster IgG Isotype Control (eBio299Arm), PE-Cyanine7, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

9E12

Immunogen

PLFZ peptide

Conjugate

PE-Cyanine7 PE-Cyanine7 PE-Cyanine7

Excitation/Emission Max

569/780 nm View spectra spectra

Form

Liquid

Concentration

0.2 mg/mL

Amount

25 µg

Purification

Affinity chromatography

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2637114

Product Specific Information

This 9E12 monoclonal antibody reacts with human and mouse promyelocytic leukemia zinc finger (PLZF), a member of the BTB-POZ family of transcription factors. Expression of this transcriptional repressor in immune cells differs between mice and humans. In mice, PLZF is highly expressed in immature CD1d-resricted NKT2 and NKT1 cells, and a subset of gamma delta (Vg1.1+Vd6.3+) T cells. Studies have also demonstrated expression of PLZF in non-invariant CD1d-restricted T cells, as well as non-CD1d-restricted innate T cells. In humans, PLZF is expressed in NK cells, gamma delta T cells, as well as CD4 and CD8+ T cells. PLZF is also expressed in MR1-specific mucosal-associated invariant T cells, as well as in MHC Class II-restricted T cells that develop via a thymocyte-thymocyte interaction in humans. PLZF is expressed by innate lymphoid cells and its progenitor cells. PLZF exists as a homodimer or in complex with PLZP, and has been shown to be involved in the development of NKT cells, NK cell function, cellular quiescence, growth suppression, and maintenance of homeostatis. Finally, PLZF has been shown to inhibit gene expression induced by retinoic acid receptor.

This 9E12 antibody stains mouse thymocytes brighter than Mags.21F7.

This 9E12 antibody has been tested by intracellular staining and flow cytometric analysis of mouse thymocytes using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

This gene is a member of the Krueppel C2H2-type zinc-finger protein family and encodes a zinc finger transcription factor that contains nine Kruppel-type zinc finger domains at the carboxyl terminus. This protein is located in the nucleus, is involved in cell cycle progression, and interacts with a histone deacetylase. Specific instances of aberrant gene rearrangement at this locus have been associated with acute promyelocytic leukemia (APL). Alternate transcriptional splice variants have been characterized.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Green's luxoid; luxoid; promyelocytic leukaemia zinc finger; Promyelocytic leukemia zinc finger protein; Zinc finger and BTB domain-containing protein 16; Zinc finger protein 145; zinc finger protein 145 (Kruppel-like, expressed in promyelocytic leukemia); Zinc finger protein PLZF

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Gene Aliases: AI467657; lu; PLZF; ZBTB16; Zfp145; ZNF145

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UniProt ID: (Human) Q05516

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Entrez Gene ID: (Human) 7704, (Mouse) 235320

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Function(s)
RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription corepressor binding transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding double-stranded DNA binding transcription factor activity, sequence-specific DNA binding protein binding zinc ion binding protein domain specific binding type 2 angiotensin receptor binding identical protein binding protein homodimerization activity metal ion binding RNA polymerase II distal enhancer sequence-specific DNA binding transcriptional repressor activity, RNA polymerase II distal enhancer sequence-specific binding protein C-terminus binding C2H2 zinc finger transcription factor
Process(es)
negative regulation of transcription from RNA polymerase II promoter skeletal system development mesonephros development regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter apoptotic process central nervous system development cell proliferation negative regulation of cell proliferation embryonic pattern specification anterior/posterior pattern specification regulation of gene expression positive regulation of macromolecule biosynthetic process protein ubiquitination hemopoiesis myeloid cell differentiation positive regulation of chondrocyte differentiation protein localization to nucleus embryonic hindlimb morphogenesis forelimb morphogenesis hindlimb morphogenesis embryonic digit morphogenesis positive regulation of apoptotic process ossification involved in bone maturation regulation of cell differentiation positive regulation of fat cell differentiation negative regulation of myeloid cell differentiation positive regulation of ossification negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter male germ-line stem cell asymmetric division positive regulation of NK T cell differentiation cartilage development positive regulation of multicellular organismal process positive regulation of cartilage development regulation of multicellular organismal development transcription from RNA polymerase II promoter embryonic limb morphogenesis
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