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Invitrogen
Phospho-S6 (Ser235, Ser236) Polyclonal Antibody
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FIGURE: 1 / 3
Phospho-S6 (Ser235, Ser236) Antibody (44-922G) in WB
Western blot analysis was performed on whole cell extracts (20 µg lysate) of HeLa (Lane 1), HeLa treated for 20 minutes with 200 nM of PMA (Lane 2), A549 (lane 3), Serum Starved A549 (lane 4) and A549 Serum Starved for overnight followed by Serum Released (lane 5). The blots were probed with Anti-Ribosomal Protein S6 (pS235)/(pS236) Rabbit Polyclonal Antibody (Product # 44-922G, 1:500 dilution) and detected... View More
Product Details
44-922G
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human RPS6 that contains serines 235 and 236. The sequence is conserved in mouse and rat.
Conjugate
Form
Liquid
Purification
Antigen affinity chromatography
Storage buffer
Dulbecco's PBS, pH 7.3, with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a cytoplasmic ribosomal protein that is a component of the 40S subunit. The protein belongs to the S6E family of ribosomal proteins. It is the major substrate of protein kinases in the ribosome, with subsets of five C-terminal serine residues phosphorylated by different protein kinases. Phosphorylation is induced by a wide range of stimuli, including growth factors, tumor-promoting agents, and mitogens. Dephosphorylation occurs at growth arrest. The protein may contribute to the control of cell growth and proliferation through the selective translation of particular classes of mRNA. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 40S ribosomal protein S6; OTTHUMP00000021121; OTTHUMP00000021122; Phosphoprotein NP33; ribosomal protein S6; RP11-513M16.6; RS6; Small ribosomal subunit protein eS6; unnamed protein product
Gene Aliases: eS6; OK/SW-cl.2; RPS6; S6
UniProt ID: (Human) P62753
Entrez Gene ID: (Human) 6194
ribosomal small subunit assembly
cytoplasmic translation
rRNA processing
translation
positive regulation of cell proliferation
TOR signaling
response to insulin
ribosomal small subunit biogenesis
glucose homeostasis
positive regulation of apoptotic process
cellular response to ethanol
negative regulation of bicellular tight junction assembly
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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