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Invitrogen

CD369 (Clec7a, Dectin-1) Monoclonal Antibody (15E2), PerCP-eFluor™ 710, eBioscience™

3 Published Figures
6 References
View all (29) Dectin 1 (Clec7a) antibodies

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Product Details

46-9856-42

Applications
Tested Dilution
Publications

Flow Cytometry (Flow)

5 µL (0.25 µg)/test
View 6 publications 6 publications
Product Specifications

Species Reactivity

Human, Non-human primate

Published species

Human

Host/Isotype

Mouse / IgG2a, kappa

Recommended Isotype Control

Mouse IgG2a kappa Isotype Control (eBM2a), PerCP-eFluor™ 710, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

15E2

Conjugate

PerCP-eFluor™ 710 PerCP-eFluor™ 710 PerCP-eFluor™ 710

Excitation/Emission Max

482/708 nm View spectra spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with BSA

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2573910

Product Specific Information

Description: This 15E2 monoclonal antibody reacts with human and non-human primate CD369, which is also known as the Dectin-1 or beta-glucan receptor or CLEC7A. CD369 is expressed predominantly on monocytes, macrophages, and dendritic cells. Moreover, this receptor can also be detected on neutrophils and lymphocytes, as well as induced by cytokines such as IL-4 or LPS. In humans, CD369 exists as two isoforms that arise due to alternative splicing. CD369 binds beta-1,3-glucan and plays an important role in activating signal transduction involved in the innate immune response to fungal and mycobacterial infections. Studies have also shown the involvement of CD369 in dendritic cell activation.

The 15E2 monoclonal antibody has been reported to recognize both isoforms of CD369 and activate dendritic cells. It does not crossreact to mouse CD369.

Applications Reported: This 15E2 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 15E2 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CLEC7A, also known as dectin-1, is a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily and is predominantly expressed on myeloid cells. It is a small glycoprotein type II membrane receptor with an extracellular C-type lectin-like domain fold and a cytoplasmic domain with an immunoreceptor tyrosine-based activation motif (ITAM). CLEC7A functions as a pattern-recognition receptor that recognizes a variety of beta-1,3-linked and beta-1,6-linked glucans from fungi and plants, and in this way plays a role in innate immune response. Upon fungal exposure, CLEC7A activates Syk tyrosine kinase, triggering a massive oxidative burst through the formation of reactive oxygen species.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Bioinformatics

Protein Aliases: Beta-glucan receptor; C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamily member 12; C-type lectin BGRA; C-type lectin BGRB; C-type lectin BGRC; C-type lectin BGRD; C-type lectin BGRE; C-type lectin BGRF; C-type lectin BGRG; C-type lectin BGRH; C-type lectin domain family 7 member A; C-type lectin superfamily member 12; CD369; DC-associated C-type lectin 1; dectin-1; dectin-1 beta; dectin-1; C-type lectin receptor; Dendritic cell-associated C-type lectin 1; dendritic cell-associated C-type lectin-1; lectin-like receptor 1; unnamed protein product

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Gene Aliases: BGR; CANDF4; CD369; CLEC7A; CLECSF12; DECTIN1; SCARE2; UNQ539/PRO1082

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UniProt ID: (Human) Q9BXN2

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Entrez Gene ID: (Human) 64581

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Function(s)
(1->3)-beta-D-glucan binding protein binding carbohydrate binding pattern recognition receptor activity MHC protein binding identical protein binding metal ion binding
Process(es)
cell activation response to yeast detection of yeast stimulatory C-type lectin receptor signaling pathway immune system process positive regulation of cytokine production involved in immune response positive regulation of dendritic cell cytokine production cell surface pattern recognition receptor signaling pathway phagocytosis, recognition inflammatory response cell recognition positive regulation of cell proliferation carbohydrate mediated signaling positive regulation of gene expression detection of fungus positive regulation of cell migration positive regulation of protein complex assembly detection of molecule of fungal origin positive regulation of interferon-gamma production positive regulation of interleukin-1 beta production positive regulation of interleukin-10 production positive regulation of interleukin-12 production positive regulation of interleukin-2 production positive regulation of interleukin-23 production positive regulation of interleukin-6 production positive regulation of interleukin-8 production positive regulation of tumor necrosis factor production positive regulation of superoxide anion generation T cell activation defense response to protozoan regulation of I-kappaB kinase/NF-kappaB signaling positive regulation of I-kappaB kinase/NF-kappaB signaling positive regulation of MAPK cascade innate immune response leukocyte activation positive regulation of nitric oxide biosynthetic process positive regulation of phagocytosis positive regulation of calcium-mediated signaling positive regulation of lymphocyte activation positive regulation of respiratory burst antifungal innate immune response regulation of calcineurin-NFAT signaling cascade positive regulation of calcineurin-NFAT signaling cascade cellular response to molecule of fungal origin positive regulation of monocyte chemotactic protein-1 production positive regulation of wound healing apoptotic signaling pathway positive regulation of cytokine production involved in inflammatory response positive regulation of cell maturation positive regulation of T-helper 17 type immune response
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