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Invitrogen
CD369 (Clec7a, Dectin-1) Monoclonal Antibody (15E2), Functional Grade, eBioscience™
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Product Details
16-9856-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Non-human primate
Host/Isotype
Mouse
/ IgG2a, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
15E2
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
no preservative
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: This 15E2 monoclonal antibody reacts with human and non-human primate CD369, which is also known as the Dectin-1 or beta-glucan receptor or CLEC7A. CD369 is expressed predominantly on monocytes, macrophages, and dendritic cells. Moreover, this receptor can also be detected on neutrophils and lymphocytes, as well as induced by cytokines such as IL-4 or LPS. In humans, CD369 exists as two isoforms that arise due to alternative splicing. CD369 binds beta-1,3-glucan and plays an important role in activating signal transduction involved in the innate immune response to fungal and mycobacterial infections. Studies have also shown the involvement of CD369 in dendritic cell activation.
The 15E2 monoclonal antibody has been reported to recognize both isoforms of CD369 and activate dendritic cells. It does not crossreact to mouse CD369.
Applications Reported: The 15E2 antibody has been reported to activate dendritic cells.
Applications Tested: This 15E2 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Storage and handling: Use in a sterile environment.
Filtration: 0.2 µm post-manufacturing filtered.
Purity: Greater than 90%, as determined by SDS-PAGE.
Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.
Aggregation: Less than 10%, as determined by HPLC.
Target Information
CLEC7A, also known as dectin-1, is a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily and is predominantly expressed on myeloid cells. It is a small glycoprotein type II membrane receptor with an extracellular C-type lectin-like domain fold and a cytoplasmic domain with an immunoreceptor tyrosine-based activation motif (ITAM). CLEC7A functions as a pattern-recognition receptor that recognizes a variety of beta-1,3-linked and beta-1,6-linked glucans from fungi and plants, and in this way plays a role in innate immune response. Upon fungal exposure, CLEC7A activates Syk tyrosine kinase, triggering a massive oxidative burst through the formation of reactive oxygen species.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Beta-glucan receptor; C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamily member 12; C-type lectin BGRA; C-type lectin BGRB; C-type lectin BGRC; C-type lectin BGRD; C-type lectin BGRE; C-type lectin BGRF; C-type lectin BGRG; C-type lectin BGRH; C-type lectin domain family 7 member A; C-type lectin superfamily member 12; CD369; DC-associated C-type lectin 1; dectin-1; dectin-1 beta; dectin-1; C-type lectin receptor; Dendritic cell-associated C-type lectin 1; dendritic cell-associated C-type lectin-1; lectin-like receptor 1; unnamed protein product
Gene Aliases: BGR; CANDF4; CD369; CLEC7A; CLECSF12; DECTIN1; SCARE2; UNQ539/PRO1082
UniProt ID: (Human) Q9BXN2
Entrez Gene ID: (Human) 64581
cell activation
response to yeast
detection of yeast
stimulatory C-type lectin receptor signaling pathway
immune system process
positive regulation of cytokine production involved in immune response
positive regulation of dendritic cell cytokine production
cell surface pattern recognition receptor signaling pathway
phagocytosis, recognition
inflammatory response
cell recognition
positive regulation of cell proliferation
carbohydrate mediated signaling
positive regulation of gene expression
detection of fungus
positive regulation of cell migration
positive regulation of protein complex assembly
detection of molecule of fungal origin
positive regulation of interferon-gamma production
positive regulation of interleukin-1 beta production
positive regulation of interleukin-10 production
positive regulation of interleukin-12 production
positive regulation of interleukin-2 production
positive regulation of interleukin-23 production
positive regulation of interleukin-6 production
positive regulation of interleukin-8 production
positive regulation of tumor necrosis factor production
positive regulation of superoxide anion generation
T cell activation
defense response to protozoan
regulation of I-kappaB kinase/NF-kappaB signaling
positive regulation of I-kappaB kinase/NF-kappaB signaling
positive regulation of MAPK cascade
innate immune response
leukocyte activation
positive regulation of nitric oxide biosynthetic process
positive regulation of phagocytosis
positive regulation of calcium-mediated signaling
positive regulation of lymphocyte activation
positive regulation of respiratory burst
antifungal innate immune response
regulation of calcineurin-NFAT signaling cascade
positive regulation of calcineurin-NFAT signaling cascade
cellular response to molecule of fungal origin
positive regulation of monocyte chemotactic protein-1 production
positive regulation of wound healing
apoptotic signaling pathway
positive regulation of cytokine production involved in inflammatory response
positive regulation of cell maturation
positive regulation of T-helper 17 type immune response
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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