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Invitrogen
CD304 (Neuropilin-1) Monoclonal Antibody (TNKUSOHA), APC-eFluor™ 780, eBioscience™
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FIGURE: 1 / 1
CD304 (Neuropilin-1) Antibody (47-3049-42) in Flow
Staining of normal human peripheral blood cells with Anti-Human CD123 PE (Product # 12-1239-42) and Mouse IgG1 K Isotype Control APC-eFluor® 780 (Product # 47-4714-82) (left) or Anti-Human CD304 APC-eFluor® 780 (right). Cells in the monocyte gate were used for analysis.
Product Details
47-3049-42
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
TNKUSOHA
Conjugate
Excitation/Emission Max
756/785 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with BSA
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The TNKUSOHA monoclonal antibody recognizes human CD304, which is also known as BDCA-4 and Neuropilin-1. Like CD303, the CD304 antigen is expressed on human plasmacytoid dendritic cells in normal blood and bone marrow. CD304 can also be detected on T follicular helper cells in inflamed tonsils, as well as on some B-cell acute lymphoblastic leukemias. In addition, CD304 is expressed on numerous non-hematopoietic cell types, including neurons, endothelial, and tumor cells. Plasmacytoid dendritic cells play a key role in the innate immune response against viral pathogens and tumors.
Applications Reported: This TNKUSOHA antibody has been reported for use in flow cytometric analysis.
Applications Tested: This TNKUSOHA antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
APC-eFluor® 780 emits at 780 nm and is excited with the Red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.
Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 633-647 nm; Emission: 780 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Neuropilin 1 is a 923 amino acid containing membrane bound coreceptor to a tyrosine kinase receptor belonging to the neuropilin family with two CUB domains, two F5/8 type C domains and a MAM domain. Neuropilin 1 forms a heterodimer with NRP2 (Neuropilin 2) and is known to bind to plexin (PLXNB1) to form semaphorin receptors. Expressed mostly in the nervous system this receptor is implicated in a wide range of functions including in the development of the cardiovascular system, angiogenesis, repulsive axon guidance, formation of certain neuronal circuits, cell survival, control of neuronal migration and organogenesis outside the nervous system. This semaphorin receptor functions as receptors for both the semaphorins and vascular endothelial growth factor and is also a regulator of VEGF-induced angiogenesis. Neuropilin 1 may be involved in aggressive advanced prostate carcinoma and in the progression of breast cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD304; Neuropilin; Neuropilin-1; Neuropilin1; NRP 1; sNRP 1; sNRP1; soluble NRP 1; soluble NRP1; transmembrane receptor; Vascular endothelial cell growth factor 165 receptor
Gene Aliases: BDCA4; CD304; NP1; NRP; NRP1; VEGF165R
UniProt ID: (Human) O14786
Entrez Gene ID: (Human) 8829
angiogenesis
patterning of blood vessels
neuron migration
positive regulation of endothelial cell proliferation
sprouting angiogenesis
cell migration involved in sprouting angiogenesis
signal transduction
cell-cell signaling
axon guidance
axonal fasciculation
response to wounding
organ morphogenesis
positive regulation of endothelial cell migration
positive regulation of smooth muscle cell migration
dendrite development
facial nerve structural organization
trigeminal nerve structural organization
vestibulocochlear nerve structural organization
nerve development
branchiomotor neuron axon guidance
gonadotrophin-releasing hormone neuronal migration to the hypothalamus
retinal ganglion cell axon guidance
cellular response to hepatocyte growth factor stimulus
endothelial cell chemotaxis
cellular response to vascular endothelial growth factor stimulus
ventral trunk neural crest cell migration
VEGF-activated neuropilin signaling pathway
otic placode formation
negative regulation of neuron apoptotic process
platelet-derived growth factor receptor signaling pathway
vascular endothelial growth factor receptor signaling pathway
hepatocyte growth factor receptor signaling pathway
positive regulation of axon extension involved in axon guidance
negative regulation of axon extension involved in axon guidance
artery morphogenesis
axon extension involved in axon guidance
positive regulation of peptidyl-tyrosine phosphorylation
positive chemotaxis
positive regulation of cytokine activity
axonogenesis involved in innervation
regulation of vesicle-mediated transport
dichotomous subdivision of terminal units involved in salivary gland branching
angiogenesis involved in coronary vascular morphogenesis
coronary artery morphogenesis
retina vasculature morphogenesis in camera-type eye
renal artery morphogenesis
sympathetic ganglion development
trigeminal ganglion development
positive regulation of ERK1 and ERK2 cascade
semaphorin-plexin signaling pathway
commissural neuron axon guidance
regulation of retinal ganglion cell axon guidance
endothelial tip cell fate specification
sensory neuron axon guidance
sympathetic neuron projection extension
sympathetic neuron projection guidance
neural crest cell migration involved in autonomic nervous system development
toxin transport
semaphorin-plexin signaling pathway involved in neuron projection guidance
semaphorin-plexin signaling pathway involved in axon guidance
positive regulation of retinal ganglion cell axon guidance
VEGF-activated neuropilin signaling pathway involved in axon guidance
protein localization to early endosome
facioacoustic ganglion development
dorsal root ganglion morphogenesis
negative regulation of extrinsic apoptotic signaling pathway
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