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Invitrogen

CD304 (Neuropilin-1) Monoclonal Antibody (TNKUSOHA), PerCP-eFluor™ 710, eBioscience™

View all (52) Neuropilin 1 (CD304) antibodies

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Product Details

46-3049-42

Applications
Tested Dilution
Publications

Flow Cytometry (Flow)

5 µL (0.06 µg)/test
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PerCP-eFluor™ 710, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

TNKUSOHA

Conjugate

PerCP-eFluor™ 710 PerCP-eFluor™ 710 PerCP-eFluor™ 710

Excitation/Emission Max

482/708 nm View spectra spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with BSA

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2573743

Product Specific Information

Description: The TNKUSOHA monoclonal antibody recognizes human CD304, which is also known as BDCA-4 and Neuropilin-1. Like CD303, the CD304 antigen is expressed on human plasmacytoid dendritic cells in normal blood and bone marrow. CD304 can also be detected on T follicular helper cells in inflamed tonsils, as well as on some B-cell acute lymphoblastic leukemias. In addition, CD304 is expressed on numerous non-hematopoietic cell types, including neurons, endothelial, and tumor cells. Plasmacytoid dendritic cells play a key role in the innate immune response against viral pathogens and tumors.

Applications Reported: This TNKUSOHA antibody has been reported for use in flow cytometric analysis.

Applications Tested: This TNKUSOHA antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Neuropilin 1 is a 923 amino acid containing membrane bound coreceptor to a tyrosine kinase receptor belonging to the neuropilin family with two CUB domains, two F5/8 type C domains and a MAM domain. Neuropilin 1 forms a heterodimer with NRP2 (Neuropilin 2) and is known to bind to plexin (PLXNB1) to form semaphorin receptors. Expressed mostly in the nervous system this receptor is implicated in a wide range of functions including in the development of the cardiovascular system, angiogenesis, repulsive axon guidance, formation of certain neuronal circuits, cell survival, control of neuronal migration and organogenesis outside the nervous system. This semaphorin receptor functions as receptors for both the semaphorins and vascular endothelial growth factor and is also a regulator of VEGF-induced angiogenesis. Neuropilin 1 may be involved in aggressive advanced prostate carcinoma and in the progression of breast cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

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Bioinformatics

Protein Aliases: alternatively spliced; lacks exon 16; CD304; Neuropilin; Neuropilin-1; Neuropilin1; NRP 1; receptor for VEGF165 and semaphorins class 3; sNRP 1; sNRP1; soluble NRP 1; soluble NRP1; transmembrane receptor; unnamed protein product; Vascular endothelial cell growth factor 165 receptor; VEGF 165 receptor

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Gene Aliases: BDCA4; CD304; NP1; NRP; NRP1; VEGF165R

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UniProt ID: (Human) O14786

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Entrez Gene ID: (Human) 8829

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Function(s)
vascular endothelial growth factor-activated receptor activity GTPase activator activity protein binding heparin binding coreceptor activity semaphorin receptor activity growth factor binding protein kinase binding cytokine binding vascular endothelial growth factor binding metal ion binding
Process(es)
angiogenesis patterning of blood vessels vasculogenesis neural crest cell migration neuron migration positive regulation of endothelial cell proliferation sprouting angiogenesis cell migration involved in sprouting angiogenesis outflow tract septum morphogenesis substrate-dependent cell migration, cell extension signal transduction integrin-mediated signaling pathway cell-cell signaling nervous system development axon guidance axonal fasciculation heart development motor neuron axon guidance response to wounding organ morphogenesis positive regulation of endothelial cell migration positive regulation of smooth muscle cell migration dendrite development facial nerve structural organization trigeminal nerve morphogenesis trigeminal nerve structural organization vestibulocochlear nerve structural organization branchiomotor neuron axon guidance gonadotrophin-releasing hormone neuronal migration to the hypothalamus cell differentiation negative regulation of axon extension regulation of vascular endothelial growth factor receptor signaling pathway retinal ganglion cell axon guidance regulation of Cdc42 protein signal transduction cellular response to hepatocyte growth factor stimulus endothelial cell chemotaxis cellular response to vascular endothelial growth factor stimulus ventral trunk neural crest cell migration vascular endothelial growth factor signaling pathway neuropilin signaling pathway VEGF-activated neuropilin signaling pathway positive regulation of phosphorylation negative regulation of neuron apoptotic process blood vessel endothelial cell migration endothelial cell migration positive regulation of angiogenesis viral entry into host cell platelet-derived growth factor receptor signaling pathway vascular endothelial growth factor receptor signaling pathway hepatocyte growth factor receptor signaling pathway sympathetic nervous system development neuron development axon extension regulation of axon extension involved in axon guidance positive regulation of axon extension involved in axon guidance negative regulation of axon extension involved in axon guidance artery morphogenesis axon extension involved in axon guidance positive chemotaxis positive regulation of filopodium assembly positive regulation of stress fiber assembly positive regulation of focal adhesion assembly axonogenesis involved in innervation regulation of vesicle-mediated transport dichotomous subdivision of terminal units involved in salivary gland branching angiogenesis involved in coronary vascular morphogenesis coronary artery morphogenesis retina vasculature development in camera-type eye retina vasculature morphogenesis in camera-type eye renal artery morphogenesis sympathetic ganglion development trigeminal ganglion development positive regulation of ERK1 and ERK2 cascade semaphorin-plexin signaling pathway commissural neuron axon guidance positive regulation of cell migration involved in sprouting angiogenesis endothelial tip cell fate specification sensory neuron axon guidance motor neuron migration sympathetic neuron projection extension sympathetic neuron projection guidance postsynapse organization regulation of plasma membrane bounded cell projection organization basal dendrite development basal dendrite arborization positive regulation of substrate adhesion-dependent cell spreading neural crest cell migration involved in autonomic nervous system development protein localization to early endosome facioacoustic ganglion development dorsal root ganglion morphogenesis otic placode development negative regulation of extrinsic apoptotic signaling pathway
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