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CD144 (VE-cadherin) Monoclonal Antibody (16B1), Alexa Fluor™ 700, eBioscience™
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FIGURE: 1 / 35
CD144 (VE-cadherin) Antibody (56-1449-41) in Flow
Human Umbilical Endothelial Cells (HUVEC) were stained with Mouse IgG1 kappa Isotype Control, Alexa Fluor 700 (Product # 56-4714-80) (blue histogram) or CD144 (VE-cadherin) Monoclonal Antibody, Alexa Fluor 700 (purple histogram). Total viable cells were used for analysis, as determined by Fixable Viability Dye eFluor 450 (Product # 65-0863-18).
Product Details
56-1449-41
Applications
Tested Dilution
Publications
Product Specifications
Published species
Non-human primate
Host/Isotype
Mouse
/ IgG1
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
16B1
Conjugate
Excitation/Emission Max
696/719 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Wet ice
RRID
Product Specific Information
Description: The 16B1 antibody reacts with human CD144, also known as VE-cadherin and cadherin-5. The cadherin family of receptors, which are calcium-dependent adhesion molecules, is known to be involved in homophilic cell interactions. VE-cadherin, which is 140 kDa, is localized at the intercellular boundaries of endothelial cells in blood and lymphatic vessels in several tissues. It is thought to play a role in vascular permeability and remodeling.
Applications Reported: This 16B1 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This 16B1 antibody has been pre-diluted and tested by flow cytometric analysis of Human Umbilical Vein Endothelial Cells (HUVEC). This may be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Alexa Fluor 700 emits at 723 nm and can be excited with the red laser (633-647 nm). Most instruments will require a 685 LP mirror and 710/20 band pass filter. Please make sure that your instrument is capable of detecting this fluorochrome.
Excitation: 633-647 nm; Emission: 723 nm; Laser: Red Laser
Target Information
VE-cadherin is a member of the cadherin superfamily that is located in a six-cadherin cluster in a region on the long arm of chromosome 16 and is involved in loss of heterozygosity events in breast and prostate cancer. VE-cadherin protein is a calcium-dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Functioning as a classic cadherin by imparting to cells the ability to adhere in a homophilic manner, VE-cadherin may play an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. An alternative splice variant has been described but the full length sequence of VE-cadherin has not been determined.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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