Search
Invitrogen
CD144 (VE-cadherin) Monoclonal Antibody (16B1), Alexa Fluor™ 488, eBioscience™
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Additional Information:
{{banner.description}}
FIGURE: 1 / 31
CD144 (VE-cadherin) Antibody (53-1449-42) in Flow
Staining of Human Umbilical Vein Endothelial Cells (HUVEC) with Mouse IgG1 K Isotype Control Alexa Fluor® 488 (Product # 53-4714-42) (blue histogram) or Anti-Human CD144 (VE-Cadherin) Alexa Fluor® 488 (purple histogram). Total viable cells were used for analysis.
Product Details
53-1449-42
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human,
Mouse
Host/Isotype
Mouse
/ IgG1
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
16B1
Conjugate
Excitation/Emission Max
499/520 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with BSA
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The 16B1 antibody reacts with human CD144, also known as VE-cadherin and cadherin-5. The cadherin family of receptors, which are calcium-dependent adhesion molecules, is known to be involved in homophilic cell interactions. VE-cadherin, which is 140 kDa, is localized at the intercellular boundaries of endothelial cells in blood and lymphatic vessels in several tissues. It is thought to play a role in vascular permeability and remodeling.
Applications Reported: This 16B1 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This 16B1 antibody has been pre-titrated and tested by flow cytometric analysis of Human Umbilical Vein Endothelial Cells (HUVEC). This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
VE-cadherin is a member of the cadherin superfamily that is located in a six-cadherin cluster in a region on the long arm of chromosome 16 and is involved in loss of heterozygosity events in breast and prostate cancer. VE-cadherin protein is a calcium-dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Functioning as a classic cadherin by imparting to cells the ability to adhere in a homophilic manner, VE-cadherin may play an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. An alternative splice variant has been described but the full length sequence of VE-cadherin has not been determined.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
Bioinformatics
Protein Aliases: 7B4 antigen; cadherin 5, type 2 (vascular endothelium); cadherin 5, type 2, VE-cadherin (vascular epithelium); Cadherin-5; CD144; cd144 antigen; endothelial-specific cadherin; unnamed protein product; Vascular endothelial cadherin; VE-cadherin
Gene Aliases: 7B4; CD144; CDH5
UniProt ID: (Human) P33151
Entrez Gene ID: (Human) 1003
receptor binding
calcium ion binding
protein binding
beta-catenin binding
protein phosphatase binding
receptor signaling complex scaffold activity
vascular endothelial growth factor receptor 2 binding
ion channel binding
cadherin binding
metal ion binding
fibrinogen binding
BMP receptor binding
protein binding involved in cell-cell adhesion
protein tyrosine kinase binding
cell morphogenesis
endothelial cell morphogenesis
blood vessel maturation
cell migration involved in sprouting angiogenesis
cellular calcium ion homeostasis
cell-cell junction assembly
cell adhesion
homophilic cell adhesion via plasma membrane adhesion molecules
cell surface receptor signaling pathway
transforming growth factor beta receptor signaling pathway
negative regulation of cell proliferation
positive regulation of gene expression
calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules
cell migration
positive regulation of cell migration
positive regulation of BMP signaling pathway
negative regulation of microtubule polymerization
positive regulation of protein complex assembly
adherens junction organization
maintenance of blood-brain barrier
regulation of vascular permeability
blood vessel endothelial cell migration
cell-cell adhesion mediated by cadherin
positive regulation of angiogenesis
negative regulation of inflammatory response
bicellular tight junction assembly
cell-cell adhesion
protein localization to bicellular tight junction
positive regulation of intracellular signal transduction
positive regulation of establishment of endothelial barrier
regulation of establishment of cell polarity
negative regulation of endothelial cell apoptotic process
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support