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RELM alpha Monoclonal Antibody (DS8RELM), Alexa Fluor™ 700, eBioscience™
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FIGURE: 1 / 6
RELM alpha Antibody (56-5441-82) in Flow
C57BL/6 mouse resident peritoneal macrophages were stained intracellularly, using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol, with F4/80 Monoclonal Antibody, FITC (Product # 11-4801-82) and 0.125 µg of Rat IgG1 kappa Isotype Control, Alexa Fluor 700 (Product # 56-4301-80) (left) or 0.125 µg of RELM alpha Monoclonal Antibody, Alexa Fluor 700 (right). Total cells were used for analysis.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
56-5441-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Human,
Mouse
Host/Isotype
Rat
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
DS8RELM
Immunogen
Recombinant mouse RELM alpha protein
Conjugate
Alexa Fluor™ 700
Alexa Fluor™ 700
Alexa Fluor™ 700
Excitation/Emission Max
696/719 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: This DS8RELM monoclonal antibody recognizes mouse RELM alpha, also known as FIZZ1. This antibody was shown not to cross-react with mouse RELM gamma.
Applications Reported: This DS8RELM antibody has been reported for use in intracellular staining followed by flow cytometric analysis.
Applications Tested: This DS8RELM antibody has been tested by intracellular staining followed by flow cytometric analysis of resident peritoneal macrophages using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Please refer to "Staining Intracellular Antigens for Flow Cytometry, Protocol A: Two step protocol for intracellular (cytoplasmic) proteins" located at www.thermofisher.com/flowprotocols . This may be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Alexa Fluor 700 emits at 723 nm and can be excited with the red laser (633-647 nm). Most instruments will require a 685 LP mirror and 710/20 band pass filter. Please make sure that your instrument is capable of detecting this fluorochrome.
Excitation: 633-647 nm; Emission: 723 nm; Laser: Red Laser.
Target Information
RELM alpha, also known as Resistin-like alpha or FIZZ1, is a small cytokine (88 aminoacids, MW 9.4 kDa) produced mostly by macrophages. Its highest constitutive expression has been observed in small peritoneal macrophages, alveolar macrophages and adipose tissue macrophages. It is strongly induced by IL-4 and IL-13. Together with Arginase 1 and Ym-1, RELM alpha is considered one of the hallmarks of the M2 polarization of mouse macrophages, however, macrophages polarized with IL-10 (M2c) express neither RELM alpha nor Arginase 1. Also, the expression patterns of RELM alpha and Arginase 1 in untreated tissue macrophages significantly differ. Elevated levels of RELM alpha may indicate parasitic infection. Humans do not have RELM alpha gene but human Resistin resembles mouse RELM alpha in respect to sequence, tissue distribution and function. Other members of RELM family in mouse: RELM beta, RELM gamma and Resistin display different tissue distribution and functional properties. RELM alpha plays an important role in parasite infection, mucosal inflammation and allergy.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: cysteine-rich secreted protein A12-gamma; cysteine-rich secreted protein FIZZ1; FIZZ2; found in inflammatory zone 1; HXCP2; hypoxia-induced mitogenic factor; parasite-induced macrophage novel gene 1 protein; PMNG1; FIZZ1; RELM-beta; RELMalpha; FIZZ1; RELMb; RELMbeta; similar to cysteine-rich secreted A12-alpha protein; unnamed protein product
Gene Aliases: 1810019L16Rik; Fizz-1; Fizz1; HIMF; RELM-alpha; RELMa; RELMalpha; Xcp2
UniProt ID: (Mouse) Q9EP95
Entrez Gene ID: (Mouse) 57262
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