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Phospho-Cyclin B1 (Ser126) Polyclonal Antibody

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Phospho-Cyclin B1 (Ser126) Polyclonal Antibody

Product Details

600-401-464

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500- 1:2,000
-

ELISA (ELISA)

1:50,000
-

Immunoprecipitation (IP)

1:100-1:1,000
-
Product Specifications

Species Reactivity

Dog, Chimpanzee, Hamster, Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 115-140 of Human Cyclin B1 protein.
3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

2.01 mg/mL

Amount

100 µg

Purification

Affinity chromatography

Storage buffer

0.02M potassium phosphate, pH 7.2, with 0.15M NaCl

Contains

0.01% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

This antibody is specific for phosphorylated human Cyclin B1 protein at the pS126 residue. BLAST analysis indicates 100 % homology of the immunizing sequence with Cyclin B1 from human. Cross reactivity may occur with Cyclin B1 from rat, dog and chimpanzee as only a single amino acid change was detected within the immunogen sequence from these sources (91% homology). Partial reactivity may also occur with Cyclin B1 from mouse and hamster where two amino acids changes was noted (83% homology). Minimal reactivity is expected with the non-phosphorylated form of the protein.

Target Information

Cyclins bind to and regulate the activity of the Cyclin dependent protein kinases (CDKs). Cyclin B1 or CCNB1 is a regulatory protein involved in mitosis. It is essential for the control of the cell cycle at the G2/M transition. Cyclin B1 complexes with p34 (cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. Cyclin B1 is not ubiquitinated during G2/M phase, resulting in its steady accumulation during G2 phase, followed by abrupt APC dependent destruction at the end of mitosis. Destruction of Cyclin B1 is required for cell cycle progression. Cyclin B1 is overexpressed in various cancers, including breast, prostate, and non-small cell lung cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: cyclin B1; cyclin B1, related sequence 1; cyclin B1, related sequence 13; G2/mitotic-specific cyclin B1; G2/mitotic-specific cyclin-B1; OTTHUMP00000221981; OTTHUMP00000223023; unnamed protein product

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Gene Aliases: Ccn-2; CCNB; CCNB1; Ccnb1-rs1; Ccnb1-rs13; Cycb; Cycb-4; Cycb-5; CycB1; Cycb1-rs1; I79_013589

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UniProt ID: (Human) P14635, (Chinese hamster) Q08301, (Rat) P30277, (Mouse) P24860

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Entrez Gene ID: (Dog) 608420, (Human) 891, (Chinese hamster) 100770842, (Rat) 25203, (Mouse) 268697

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Function(s)
patched binding protein binding cyclin-dependent protein serine/threonine kinase regulator activity protein kinase binding ubiquitin-like protein ligase binding cyclin-dependent protein serine/threonine kinase activator activity kinase activity protein complex binding histone kinase activity kinase activator
Process(es)
G1/S transition of mitotic cell cycle G2/M transition of mitotic cell cycle oocyte maturation in utero embryonic development mitotic spindle organization mitotic metaphase plate congression spermatogenesis response to xenobiotic stimulus response to mechanical stimulus response to toxic substance negative regulation of gene expression positive regulation of G2/M transition of mitotic cell cycle positive regulation of mRNA 3'-end processing tissue regeneration mitotic cell cycle phase transition positive regulation of cell cycle positive regulation of mitotic cell cycle response to DDT positive regulation of fibroblast proliferation digestive tract development cell division positive regulation of attachment of spindle microtubules to kinetochore ventricular cardiac muscle cell development positive regulation of cardiac muscle cell proliferation regulation of chromosome condensation protein-containing complex assembly cellular response to iron(III) ion cellular response to fatty acid cellular response to hypoxia regulation of mitotic cell cycle spindle assembly checkpoint positive regulation of mitochondrial ATP synthesis coupled electron transport negative regulation of protein phosphorylation protein complex assembly protein phosphorylation cell cycle mitotic nuclear division positive regulation of histone phosphorylation histone H3-S10 phosphorylation involved in chromosome condensation
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