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Connexin 43 Polyclonal Antibody

View all (42) Connexin 43 antibodies

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Product Details

600-401-MJ2S

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
-

Immunohistochemistry (IHC)

1:200
-

Immunocytochemistry (ICC/IF)

15 µg/mL
-

ELISA (ELISA)

1:10,000-1:15,000
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Anti-Connexin 43 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal portion of mouse connexin 43 conjugated to Keyhole Limpet Hemocyanin (KLH).

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.92 mg/mL

Amount

23 µg

Purification

Affinity chromatography

Storage buffer

0.02M potassium phosphate, pH 7.2, with 0.15M NaCl

Contains

0.01% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

This affinity purified antibody is directed against rat connexin 43. A BLAST analysis was used to suggest cross-reactivity with the antigen based on 100% homology with the immunizing sequence to rat, black bear, European rabbit, wild boar, Macaca fascicularis, and Chlorocebus aethiops.

Target Information

Connexin 43 (Cx43) is a member of the gap junction protein family. Connexins assemble as a hexamer and are transported to the plasma membrane to create a hemichannel that can associate with hemichannels on nearby cells to create cell-to-cell channels. Clusters of these channels assemble to make gap junctions. Gap junction communication is important in development and regulation of cell growth. Phosphorylation of Cx43 is important in regulating assembly and function of gap junctions. Ser368 of Cx43 is phosphorylated by protein kinase C (PKC) after activation by phorbol esters, which decreases cell-to-cell communication. Src can interact with and phosphorylate Cx43 to alter gap junction communication. GFAP are membrane-spanning proteins that facilitate the transfer of ions and small molecules between cells. According to sequence similarities at the nucleotide and amino acid levels, the gap junction proteins are divided into two categories, alpha and beta. Connexin 43 is the major protein of gap junctions in the heart, and gap junctions are thought to have a crucial role in the synchronized contraction of the heart and in embryonic development. Connexin 43 is also targeted by several protein kinases that regulate myocardial cell-cell coupling. A related intron-less connexin 43 pseudogene, GJA1P, has been mapped to chromosome 5. Mutations in the GFAP gene cause X-linked Charcot-Marie-Tooth disease, an inherited peripheral neuropathy, oculodentodigital dysplasia and heart malformations. Alternatively spliced transcript variants of GFAP have been found.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: alpha 1 connexin; connexin 43; Connexin-43; Cx-43; Cx43; CXA1; DFNB38; Gap junction 43 kDa heart protein; Gap junction alpha-1 protein; gap junction membrane channel protein alpha 1; gap junction protein, alpha 1, 43kDa; GJA1; ODD; ODOD; SDTY3; Syndactyly type III; unnamed protein product

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Gene Aliases: AU042049; AVSD3; AW546267; CMDR; Cnx43; connexin43; CX43; Cx43alpha1; Cxn-43; EKVP; EKVP3; Gja-1; GJA1; GJAL; HLHS1; HSS; Npm1; ODDD; PPKCA

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UniProt ID: (Human) P17302, (Mouse) P23242, (Rat) P08050

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Entrez Gene ID: (Human) 2697, (Mouse) 14609, (Rat) 24392

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Function(s)
gap junction channel activity protein binding beta-catenin binding ion transmembrane transporter activity efflux transmembrane transporter activity tubulin binding transmembrane transporter activity glutathione transmembrane transporter activity alpha-tubulin binding gap junction hemi-channel activity gap junction channel activity involved in cardiac conduction electrical coupling gap junction channel activity involved in cell communication by electrical coupling signal transducer activity receptor binding SH3 domain binding protein domain specific binding PDZ domain binding beta-tubulin binding connexin binding scaffold protein binding gap junction
Process(es)
establishment of mitotic spindle orientation cardiac conduction system development cell communication signal transduction cell-cell signaling spermatogenesis heart development protein localization positive regulation of gene expression cell communication by electrical coupling glutamate secretion gap junction assembly negative regulation of cell growth negative regulation of gonadotropin secretion ion transmembrane transport glutathione transmembrane transport maintenance of blood-brain barrier xenobiotic transport positive regulation of I-kappaB kinase/NF-kappaB signaling cell-cell junction organization bone remodeling transmembrane transport bone development atrial cardiac muscle cell action potential cell communication by electrical coupling involved in cardiac conduction microtubule-based transport positive regulation of cold-induced thermogenesis export across plasma membrane negative regulation of trophoblast cell migration regulation of blood circulation cellular response to beta-amyloid positive regulation of vascular smooth muscle cell proliferation positive regulation of morphogenesis of an epithelium positive regulation of mesodermal cell differentiation positive regulation of stem cell proliferation osteoblast differentiation in utero embryonic development neuron migration negative regulation of endothelial cell proliferation heart looping regulation of heart rate epithelial cell maturation lens development in camera-type eye chronic inflammatory response positive regulation of glomerular filtration atrial ventricular junction remodeling epicardial cell to mesenchymal cell transition apoptotic process positive regulation of cytosolic calcium ion concentration adult heart development regulation of heart contraction negative regulation of cell proliferation response to pH vascular transport negative regulation of gene expression cell communication by chemical coupling positive regulation of cell communication by chemical coupling ATP transport regulation of bone mineralization positive regulation of insulin secretion embryonic heart tube development embryonic digit morphogenesis skeletal muscle tissue regeneration positive regulation of osteoblast differentiation positive regulation of protein catabolic process positive regulation of striated muscle tissue development positive regulation of vasoconstriction positive regulation of vasodilation regulation of bone remodeling blood vessel morphogenesis neuron projection morphogenesis protein oligomerization regulation of calcium ion transport negative regulation of cardiac muscle cell proliferation milk ejection regulation of ventricular cardiac muscle cell membrane repolarization regulation of blood vessel remodeling regulation of atrial cardiac muscle cell membrane depolarization regulation of ventricular cardiac muscle cell membrane depolarization negative regulation of wound healing cardiac conduction establishment of epithelial cell polarity negative regulation of DNA biosynthetic process regulation of bicellular tight junction assembly positive regulation of behavioral fear response endothelium development response to glucose response to fluid shear stress response to peptide hormone cellular response to mechanical stimulus
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